骨肉瘤好发于青少年,约占所有恶性骨肿瘤的35%[1].传统治疗方法虽然能有效的提高骨肉瘤患者的术后生存率,但是仍有部分患者发生肺部转移。miRNA是一种长度约为22bp的非编码单链RNA,它与靶基因3’端互补配对,降解或抑制靶基因表达,从而影响肿瘤细胞的生长、凋亡等病理过程[2-4]。已有研究表明miR-9在喉鳞癌、鼻咽癌、卵巢癌等肿瘤细胞中呈低表达水平[5-7],但它在骨肉瘤的表达

骨肉瘤（osteosarcoma,OS）是最常见的威胁青少年生命健康的高度恶性骨肿瘤[1]。骨肉瘤好发于长骨或者血运供应充足的干骺端,由于实体瘤的组织异质性和对放化疗的耐受性不同,导致骨肉瘤的肺转移率显著升高且预后较差[2]。常规的治疗手段（化学治疗、放射治疗、免疫治疗等生物治疗）很难从根本上治愈骨肉瘤,相关文献报道亦表明骨肉瘤患者在接受综合治疗后的总体生存率远不足

BACKGROUND:The mechanical index is an important method for the evaluation of the therapeutic efficiency of drug treatment for osteoporosis animal models. OBJECTIVE: To explore the effects of various drug treatments on osteoporosis through a mechanical performance test about the femoral compression of rats. METHODS: Thirty-six Wistar female rats were randomized into six groups: normal control group, model group, Dan Qiparticles group, alpha-D3 group, premarin group, ipriflavone group, with six rats in each group. Osteoporosis models were made in al groups except for the normal control group, and after modeling, the rats in different groups were treated withDan Qi particles, alpha-D3 group, premarin and ipriflavone, respectively. After 15 weeks, the rats were kiled by abdominal aortic bloodletting to take out the left and right femurs that were placed on a universal testing machine to perform a compressive test at a speed of 5 mm/min. RESULTS AND CONCLUSION:The maximum load, maximum stress, maximum displacement, maximum strain, and elastic modulus were significantly lower in the model group than the other four groups (P < 0.05). There was no difference in different mechanical parameters between alpha-D3 group and model group as wel as between Dan Qi particles group and normal control group (P> 0.05). These findings indicate that osteoporosis leads to the variation of compression mechanical properties of the femur. There are good compression mechanical properties of the femur after treatment with premarin and ipriflavone, andDan Qi particles has the best effect.

At present,the medical profession generally acknowledged the best way to treat osteosarcoma is gene therapy,which includes tumor suppressor gene therapy,antisense gene therapy,suicide gene therapy,immune gene therapy,combined gene therapy,etc.But no matter what kind of gene therapy is that the gene must have a safe carrier.Gene therapy has made a breakthrough in osteosarcoma recently.On the basis of widespread use,we should emphasize the importance of gene vectors.

Objective We sought to identify microRNA altered in multiple myelomaand determine that microRNA ratio is an predictive tool for multiple myeloma.Methods The plasma of MM patients and 30 normal controls were choosed and cofirmed,Respectively.The miRNA expression profiles were examined with Illumina Bead Chips.The re-sults were verified by qRT-PCR.The miRNA expression ratio was analysed by ROC curve,aiming to find the most pre-dictive potential of combination-type markers.Results 178 miRNAs were found to be significantly deregulated in the plasma of 24 MM patients and 30 normal controls plasma samples.miR-21 were significantly higher than in normal plasma sample,while miR-199a-5p,miR-16 and miR-125a-5p showed low expression;Furthermore,the ratio of miR-21/miR-199a-5p showed a high sensitivity and specificity for disease predicition.Conclusion These results indicate that miR-21/miR-199a-5p ratio could be used as diagnostic biomarker in multiple myeloma.