目的:探索调气活血法治疗大鼠慢性萎缩性胃炎的疗效及其对胃酸分泌影响的机制.方法:采用N-甲基-N-硝基-亚硝基胍(MNNG)自由饮用的综合造模法复制大鼠慢性萎缩性胃炎动物模型,造模成功后,分别给予调气活血方、胃复春片、蒸馏水灌胃治疗8周,处死大鼠,收集大鼠胃酸,测胃酸pH值,取大鼠胃组织,苏木素-伊红染色(HE染色)观察病理变化,免疫组织化学方法检测胃黏膜质子泵(H,K-ATPase)、ezrin蛋白表达水平.结果:调气活血方可有效治疗大鼠慢性萎缩性胃炎,显著提高大鼠胃酸分泌水平,增加胃黏膜H,K-ATPase、ezrin蛋白的表达面积,与空白模型组和胃复春组比较,差异具有统计学意义(P＜0.01,P＜0.05).结论:调气活血法可能通过提高胃黏膜ezrin蛋白表达水平,促进了H,K-ATPase在壁细胞顶膜锚定,从而促进了壁细胞酸分泌,达到治疗大鼠慢性萎缩性胃炎和延缓疾病进展的作用.

Objective To discuss the distribution characteristics of TCM syndrome and syndrome factors of functional dyspepsia so as to lay the foundation for the objectification and normalization of clinical research and provide the evidence for clinical treatment. Methods By the collection of clinical cases,200 cases of functional dyspepsia were collected,in compliance with Roman Ⅲ criteria. The factor analysis meth-od was applied. Results The factor analysis showed that functional dyspepsia is characterized as the compli-cation of deficiency and excess. The most common syndromes are:spleen deficiency and qi stagnation,spleen and stomach deficiency,liver and stomach disharmony and mixture of cold and heat. The main TCM syndrome is spleen deficiency and qi stagnation. The disease is localized in spleen,stomach and liver,in which,spleen is predominated. The factors of disease nature are qi stagnation,qi deficiency,damp,heat,yang deficiency, cold and blood stagnation,in which,qi stagnation,qi deficiency,damp,heat and cold are predominated. Con-clusion For the location of disease,the relevant factors are spleen,stomach and liver,in which,the spleen is predominated. For the nature of disease,the relevant factors are qi stagnation,qi deficiency,damp,heat,yang deficiency,cold and blood stagnation. The syndrome of the disease is characterized as the complication of de-ficiency and excess,in which spleen deficiency and qi stagnation is predominated.

Objective Through literature study,this paper explores the distribution of syndromes and syndrome elements of chronic atrophic gastritis(CAG)for future specification of the syndrome elements. Methods The authors searched for the literature of CAG,built up a literature database by Epidata3. 1 soft-ware,and analyzed the data by SPSS19. 0 software. Results 152 documents were included in this paper and 19 083 cases were reported. 74 kinds of syndromes and 20 kinds of syndrome elements were found. From two respects of frequency and proportion of the literature,it was found that the frequent syndromes of CAG are Yang deficiency of the Spleen and Stomach,Yin deficiency of the Stomach,Disharmony of the Liver and Stomach,Heat and Dampness of the Spleen and Stomach,Blood Stasis of the Stomach. Major syndrome ele-ments of disease location are Stomach,Spleen and Liver. Major syndrome elements reflecting the nature of disease are Stagnation,Deficiency(qi deficiency and yang deficiency),Dampness,Heat and Blood Stasis. Conclusions This research initially reflected the most common distribution features syndromes and syn-drome elements of CAG and it can provide reference and basis for further identified standards of this disease.