Mesenchymal stromal cells (MSCs) with its unique biological characteristics and great prospects for clinical application, become a hot research topic in many therapeutic areas including spinal cord injury. MSCs can play a neuroprotective role in the treatment of spinal cord injury by releasing trophic factors, inhibiting inflammatory response and so on, and has a broad application prospects in the treatment of spinal cord injury.

Objective:To investigate the plasticity of pedunculopontine tegrnental nucleus according to the changes of standardized uptake value(SUV) and vessicular glutamate transporters 2(VGLUT2) expression after body weight support treadmill training (BWSTT) in incompletely spinal cord injury rats.Method:Twenty-four Sprague-Dawley rats were randomly divided into the sham operated group (n=8),the untrained control group (n=8),and BWSTT treatment group (n=8).Spinal cord moderate contusion model was made at T10 segment with NYU impactor.Behavioral ability was evaluated by Basso-Beattie-Bresnahan (BBB) scale of hind limb movement.The 18F-FDG SUV of PPTg was collected by PET/CT.Furthermore,the VGLUT2 expression in PPTg were processed by immunofluorescence for quantitative analysis.Result:Comparing with the control group,BBB scores increased significantly more in the BWSTT treatment group (P＜0.05) at 7 weeks post-injury,whereas scores in BWSTT treatment group and control group are still much lower than those in sham group (P ＜ 0.05).The expression of VGLUT2 in BWSTT treatment group is much higher than that in untrained control group,and sham operated group is the highest among three groups.Conclusion:BWSTT can partially improve recovery of hind limbs locomotion.One of the mechanism is that it markedly promote neurohumoralplasticity of PPTg in rat after iSCI

Objective To investigate the protective effects of bone marrow mesenchymal stromal cells ( BMSCs ) on spinal cord injury ( SCI ) model of rats and its potential mechanism. Methods Totally 60 adult SD rats were randomly divided into 3 groups: sham group ( n = 20 ), SCI group ( n = 20 ) and BMSCs group ( n = 20 ). Model of spinal cord injury was established by modified Allen’ s assay. From the first day after injury, motor function of the rats’ hind limb was evaluated by BBB ( Basso Beattie and Bresnahan ) scores and Catwalk gait analysis and detection at 14 d. Content of myeloperoxidase ( MPO ), malondialdehyde ( MDA ), superoxide dismutase ( SOD ) in spinal cord tissue and levels of tumor necrosis factor-α ( TNF-α ), interleukin-6 ( IL-6 ), interleukin-10 ( IL-10 ) in serum were determined by ELISA analysis. Pathological changes of spinal cord tissues were observed with HE staining. Results Compared with the model group, the content of MPO in injured spinal cord at 12h after injury in BMSCs group decreased from ( 149.7 ± 28.4 ) nmol / mg to ( 116.0 ± 29.2 ) nmol / mg, MDA levels decreased from ( 58.1 ± 3.3 ) U / g to ( 41.7 ± 2.6 ) U / g, at 6 h after injury TNF-α levels in serum decreased from ( 439.8 ± 12.8 ) pg / ml to ( 314.6 ± 17.6 ) pg / ml, IL-6 levels decreased from ( 364.7 ± 16.9 ) pg / ml to ( 232.9 ± 15.2 ) pg / ml while SOD activity increased from ( 194.6 ± 14.3) U / mg to ( 362.0 ± 13.6 ) U / mg, IL-10 levels increased from ( 291.3 ± 9.8 ) pg / ml to ( 377.9 ± 17.9 ) pg / ml ( P < 0.05 ). Compared with the SCI group, BBB scores and catwalk gait analysis improved significantly at 14 d after injury in BMSCs group ( P < 0.05 ). The pathological injury of spinal cord was relieved in BMSCs group. Conclusions BMSCs administration improves the motor function after spinal cord injury. Its potential mechanism may be the inhibition of inflammatory reaction and oxidative stress reaction.

BACKGROUND:The elderly hip fracture patients with chronic obstructive pulmonary disease (COPD) have a higher postoperative mortality than those only with hip fractures. In recent years, it has become an issue of concerns. Because the mechanism is unknown, however, there are no effective clinical interventions for these patients. OBJECTIVE:To observe the effect of bone marrow stromal stem cel s (BMSCs) on the level of pulmonary inflammation in aged rats with chronic obstructive pulmonary disease (COPD) after hip fractures. METHODS:Thirty male Sprague-Dawley rats, 12 months old, were exposed to smoking for 4 months and randomized into three groups, smoking, smoking+hip fracture+normal saline, smoking+hip fracture+BMSCs groups. Animal models of hip fracture were made in the latter two groups. Twenty-four hours after hip fracture, the lower lobe and peripheral blood samples were taken from al rats in the three groups, to evaluate the pathological changes of lung tissue and detect levels of inflammatory factors in the lung tissue and peripheral blood. RESULTS AND CONCLUSION:After closed hip fracture, aged COPD rats exhibited inflammatory cel infiltration, mucus secretion, airway stenosis or occlusion;the levels of interleukin-6, tumor necrosis factor-αand interleukin-10 in the lung tissue and peripheral blood were increased. After intravenous injection of BMSCs, the pathological changes of the lung tissue were reduced, and the levels of pro-inflammatory factors, tumor necrosis factor-αand interleukin-6, decreased, but the level of anti-inflammatory factor interleukin-10 further increased, which were significantly different from those in the normal saline group (P<0.05). These findings indicate that BMSCs can relieve acute lung injury in aged COPD rats with hip fractures.

目的 探讨槲皮素对大鼠急性脊髓损伤的保护作用及其可能机制.方法 将72只雌性SD大鼠随机分为模型组(SCI组)、槲皮素组(Que组)和假手术组(Sham组),每组各24只.采用改良Allen's打击法建立脊髓损伤模型,术后评估大鼠后肢运动功能(BBB评分),酶联免疫吸附试验(Elisa)检测脊髓组织匀浆髓过氧化物酶(myeloperoxidase,MPO)活性、脂质过氧化产物(malonydialdehyde,MDA)含量及超氧化物歧化酶(superoxide dismutase,SOD)活性,取血清检测肿瘤坏死因子α (tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)、白细胞介素10(IL-10)含量.HE染色观察脊髓组织的病理变化,全血细胞分析检测血液白细胞水平变化.结果 与模型组相比,槲皮素组大鼠脊髓损伤后6h脊髓组织MPO含量由(65.5±12.3) nmol/mg降至(42.2±10.3) nmol/mg,MDA含量由(62.9±2.7) U/g降至(50.3±1.8) U/g,SOD水平由(212.4±10.3) U/mg升至(310.6±11.3) U/mg,血清TNF-α水平由(398.1±7.4) pg/ml降至(284.4±7.6) pg/ml,血清IL-6水平由(359.1±20.9) pg/ml降至(242.9±5.2) pg/ml,血清IL-10水平由(288.3±4.8) pg/ml升至(347.9±5.9) pg/ml,全血白细胞计数由(6.6±1.2)/视野降至(3.4±0.8)/视野,脊髓损伤后14 d时BBB评分由6.7±0.4升至9.1±0.7,差异均有统计学意义(P＜0.05).光镜下脊髓组织损伤程度降低.结论 槲皮素对大鼠急性脊髓损伤具有保护作用,其机制可能是通过抑制炎性因子从而调节炎症反应及自由基而实现的.

目的 观察多发伤性创伤大鼠肺组织内的变化,探讨多发性创伤后急性肺损伤的相关机制.方法 选取健康雌性SD大鼠56只,随机分为假手术组(只在颅骨钻孔,28只)和模型组(股骨干闭合骨折合并脑损伤,28只).分别在术后6h、12h、24 h、48 h及5d取大鼠右肺下叶组织,Real Time-PCR检测各个时间点Beclin-l和LC3-Ⅱ基因的动态表达.结果 在以上各个时间点,模型组Beclin-1、LC3-Ⅱ的mRNA水平较假手术组均明显上调.术后48 h模型组Beclin-1、LC3-Ⅱ的mRNA水平(7.532±0.03,10.843±0.03)明显高于假手术组(1.063±0.10,1.091±0.11),差异有统计学意义(P＜0.01).结论 自噬可能参与了股骨干骨折合并脑损伤这类严重多发伤后的急性肺损伤.