OBJECTIVE To explore the apoptosis-inducing effect of Ad-p53 plus fulvestrant in MCF-7 breast cancer cells. METHODS ER-positive MCF-7 breast cancer cells were used. MCF-7 cells were established with control, fulvestrant alone, Ad-p53 alone and Ad-p53 plus fulvestrant. The morphological changes of MCF-7 after drug combination were observed by inverted microscopy. Methyltetrazolium salt (MTS) assay was used to determine the proliferation of MCF-7 cells. The protein expressions of p53 and ER were examined by Western blot. Flow cytometry was used to detect apoptosis. RESULTS Significant inhibition of cell growth was observed by inverted microscopy. Flow cytometry revealed that the apoptosis rate of using fulvestrant alone or Ad-p53 alone was (13.3 ± 1.2) % and (14.5 ± 2.9) %. The apoptosis rate of combined group was (38.1 ± 5.9)% and it was higher than Ad-p53 or fulvestrant alone (P = 0.001, P = 0.001). MTS assay indicated that, after treating for 24, 48, 72, 96 h by Ad-p53 alone or fulvestrant alone, the inhibition of cell proliferation rate was (8.21 ± 0.54)%, (28.50 ± 1.42)%, (50.14 ± 0.78)%, (58.25 ± 2.92)% and (9.73 ± 1.68)%, (25.26 ± 0.82)%, (35.25 ± 3.94)% and (46.37 ± 2.56)% respectively. Simultaneously, the inhibition of cell proliferation rate of combined group was (12.42 ± 1.76)%, (35.20 ± 0.58)%, (62.08 ± 2.56)% and (75.43 ± 3.56)%. It further confirmed the increase of cell proliferation inhibition rate after drug combination (P < 0.05). Western blot showed that the p53 protein expression level increased obviously with Ad-p53 in MCF-7cells. Ad-p53 increased the level of ER expression while drug combination reduced the level of ER expression. CONCLUSIONS Ad-p53 plus fulvestrant can significantly inhibit cell growth and induce cell apoptosis. And there is probably some synergistic effects between both.

Objective To investigate the effect of Ad-p53 on the expression of p53 gene and the growth,cell cycle and apoptosis of MCF-7 cells. Method The changes of human breast cancer cell line MCF-7 morphology were ob-served by an inverted microscopy. MTS was applied to explore the impacts of Ad-p53 on the proliferation of MCF-7. After cells were cultured with Ad-p53 for 72h,the apoptosis rate and cell cycle were analyzed by flow cytometry. Western blot was used to detect the protein expression of p53. Result Significant changes of cell morphology in MCF-7 cells were observed by an inverted microscope. Ad-p53 could inhibit the growth of MCF-7 cells with optimal dose and best time. Flow cytometry revealed that Ad-p53 could increase the cell proliferation inhibition rate,and there was significant difference between MCF-7 group and control group. Western blotting results indicated that the p53 protein expression level in MCF-7 cells did not increase obviously with Ad-p53. Conclusion These results suggest that Ad-p53 can inhibit the cell growth and induce the cell apoptosis in p53-independent manner.

Objective:To investigate the effect of Ad-p53( p53 -expressing adenovirus)on the expression of p53 gene and the growth,cell cycle and apoptosis of MDA-MB-231 cells. Methods:The changes of human breast cancer cell line MDA-MB-231 morphology were observed by inverted microscopy. The apoptosis rate and cell cycle were analyzed by flow cytometry. Western blot was used to detect the protein expression of p53. Results:Significant changes of cell morphology in MDA-MB-231cells were observed by an inverted microscope. Flow cytometry re-vealed that Ad-p53 could increase the cell proliferation inhibition rate,and there was significant difference between MDA-MB-231 group and control group,and the proportion of MDA-MB-231 cells in S phase and G2/M phase decreased but increased in G0/G1 phase. Western blot results indicated that the p53 protein expression level in MDA-MB-231 cells increased obviously with Ad-p53. Conclusion:Ad-p53 can inhibit the cell growth and induce the cell apoptosis,which may be related to inhibition of the cell cycle and increase in the expression of wild-type p53 protein.

目的 探讨高强度聚焦超声(HIFU)治疗乳腺癌的有效性和可行性.方法 选取经穿刺活检病理确诊为乳腺浸润性导管癌患者13例,接受高强度聚焦超声治疗而后行改良根治术,对癌组织及周围正常组织分别取材,进行光镜、电镜观察及NADH-黄递酶活性检测.结果 光镜观察11例靶区内肿瘤组织仅有轻微退变,2例靶区内约40％的肿瘤组织出现凝固性坏死.电镜显示靶区内肿瘤细胞凝固性坏死,NADH-黄递酶活性检测发现靶区肿瘤染色阴性.结论 经高强度聚焦超声治疗,乳腺癌组织发生坏死,HIFU有望成为乳腺癌治疗的有效方法.