Clinical trail registration policies aims to reduce bias and improve the transparency and scientific validity of research.We investigate the instructions for authors of 258 medical journals in China to identify whether each journal adhere to trial registration policies.57 （22.1%） medical journals adhere to clinical trail registration policies,of which 50 journals provide specific requirements.We further demonstrate that the consistency and accuracy of the clinical trial registry numbers in randomized controlled trials （RCT）,and whether the journals adherence to reporting guidelines impact the likelihood of reporting trail registry number.The results indicate that medical journals have a poor compliance of reporting guidelines and clinical trail registration policies,with only 17.2%of RCTs registered.Medical journals do not sufficiently pay attention to the registration and lack of strict implementation,and journals adherence to CONSORT increase the likelihood of RCT registration.It is recommended to follow the guidelines and trail registration policies for medical journals,increase the awareness and attention to the clinical trial registration.This requires the joint participation of journal reviewers,editors and authors,to improve the transparency of research by increasing the rate of clinical trial registration.

随着新媒体技术的不断发展,媒体融合的时代已经来临,为医学期刊的发展带来了机遇与挑战.医学期刊媒体融合面临一系列的问题,要想走出困境,推动刊物发展,就必须树立数字化出版理念,培养新媒体人才,充分利用自身的优势,加强期刊品牌的建设,促进新媒体融合发展,进一步提升期刊的学术影响力和竞争力.

"微传播"时代是以微博、微信等微媒介传播活动日常化为特征的新兴媒体的时代.医学期刊是以医学和与医学相关学科为内容的情报载体,是交流医学前沿思想、沟通医学信息的桥梁和纽带.本文阐述了面对"微传播"时代的到来,医学期刊编辑需顺应时代潮流,运用微博、微信等微媒介,适应新形势下的角色转变,不断创新和沉淀,明确编辑职责,更新编辑观念,提升编辑素养,以满足时代发展赋予医学期刊编辑工作的新要求.

新媒体时代下,医药期刊面临机遇和挑战并存的情况,微信公众平台为医药期刊从纸质、互联网营销转向手机端营销提供了可能.随着越来越多医药期刊微信公众号的出现,如何创作出优质的图文消息来服务目标用户显得尤为重要.本文就医药期刊微信公众号图文消息创作思路进行探索式分析和研究,希望能给医药期刊互联网营销实践操作提供一些实用性帮助.

Objective:To establish an NIH3T3 cell line that stably expresses GFP-V12Rac1(constitutively active Rac1 fused with a GFP tag).Methods:Plasmids and lentiviral vectors containing GFP-V12Rac1 and GFP were constructed.NIH3T3 was infected with lentiviral vectors,and cells stably expressing genes of interest were selected by flow cytometry.A cell spreading assay was used to confirm that exogenous GFP-V12Rac1 was of normal function;a Boyden chamber assay was used to test the motility of established cell lines.Results:Stable cell lines expressing GFP or GFP-V12Rac1 were established.GFP-V12Rac1 promoted cell spreading.Chemotaxis of established cell lines was confirmed.Conclusion:NIH3T3 cell lines stably expressing GFP-V12Rac1 were successfully established using lentiviral methods;exogenous GFP-V12Rac1 was of normal function;chemotaxis of the cell lines was confirmed.These cell lines can be used as model cells for further study of active Rac1 targeting.