[目的]试验旨在探讨苦玄参提取物对仔猪生长性能的影响,为其在动物生产中更好地应用提供参考依据.[方法]选取体重相近的28～35日龄杜×长×大三元杂交的健康断奶仔猪250头,随机分为5组(A、B、C、D、E组),每组设5个重复,每个重复10头.其中,A、B、C组依次为苦玄参提取物高、中、低3个剂量试验组,分别在基础日粮中添加3.500、1.750和0.875 g/kg苦玄参提取物;D组在基础日粮中添加对照药物七补散4.0 g/kg;E组为空白对照组,饲喂基础日粮,预饲期7 d,正试期30 d.于试验正式开始的第0天、第15天和第30天7:00分别对各组的每一头试验猪进行空腹称重,并每天记录每个组试验猪的采食量,计算平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G).[结果]添加苦玄参提取物后,A、B、C组仔猪的体重、ADG及ADFI,与E组相比显著或极显著(P<0.05或P<0.01)增加,而料重比降低;其中苦玄参提取物中剂量组仔猪的体重、平均日增重及平均日采食量提升最明显,料重比也极显著低于D组与E组(P<0.01).[结论]苦玄参提取物对仔猪生长具有一定的促进作用,以1.750 g/kg的添加剂量添加在饲料中效果最佳.

[目的]建立苦玄参提取物中苦玄参苷ⅠA的鉴别和含量测定方法.[方法]采用薄层色谱法对苦玄参苷进行定性鉴别;采用高效液相色谱法测定提取物中苦玄参苷ⅠA的含量,色谱条件为:Waters Symmetry C18(4.6×250 mm,5μm)色谱柱,流动相为乙腈—水(35:65),流速1.0 mL/min,检测波长264 nm,柱温35℃,进样量20μL.[结果]苦玄参苷ⅠA的薄层色谱鉴别方法专属性强;苦玄参苷ⅠA在20.06~104.10μg/mL的质量浓度范围内线性关系良好(R2=0.9992),平均加样回收率为99.90%,RSD=0.53%.[结论]建立的方法专属性强,定量准确性高,适用于苦玄参提取物的质量控制.

[Objective]An inflammatory model of porcine pulmonary alveolar macrophages(PAM)induced by por-cine reproductive and respiratory syndrome virus(PRRSV)was established,and the impact of PRRSV infection on the immune system of piglets were evaluated to provided reference for the clinical diagnosis and prevention of porcine repro-ductive and respiratory syndrome(PRRS).[Method]The 30-day-old healthy weaned piglets were artificially infected with strain PRRSV-GXNN1396 to replicate the PRRS condition.The samples were collected after the piglets were killed.The histopathological changes and distribution of virus in immune organs and tissues were observed by using histopathology and RT-PCR detection technology.Enzyme activity variation such as COX-1 and COX-2 and levels of inflammatory cyto-lines including IL-6,IL-8,IL-10,IL-1β,IFN-γ,MCP-1 and TNF-α in PAM cell were detected.[Result]The results showed that the typical signs of PRRS occurred in infected weaned piglets on the 3rdday after infection of PRRSV-GXNN1396.The viruses were mainly distributed in lungs,lymph nodes,blood and tonsil.The parenchymal cells and re-lated lymphocytes in major organs of challenged piglets showed necrosis and infiltration of inflammatory cells at various degrees.In lung,kidney and spleen especially,macrophages and lymphocytes infiltrated the organs and tissues.The levels of ROS and various factors(COX-2,IL-6,IL-8,IL-10 and TNF-α)in piglets PAM cells all presented a rising trend at early PRRSV infection stage(day 3).Levels of COX-2,IL-6,IL-8 and TNF-α extremely significantly increased (P<0.01).With the increase of infection time,in PAM cell,the levels of factors such as COX-2,IL-6 and IL-8 decreased,yet TNF-α level increased.[Conclusion]An inflammatory model of porcine PAM cell induced by PRRSV infection is established,which further testifies that PRRSV mainly affects the immune organs and tissues of piglets to achieve immune suppression.In addition,it is concluded that day 3 of infection is the peak of inflammatory reaction in diseased pigs.