Objective To develop a reversed phase high performance liquid chromatographic(RP-HPLC) analysis system for hydroxysafflor yellow A(HSYA) in rat model of cold coagulation and blood stasis(CCBS),and to investigate the influence of Sappan lignum on the pharmacokinetics of HSYA in CCBS rats.Methods Rat CCBS models were randomly divided into two groups with each containing 6 animals.Rats were orally given Carthami flos extract or Carthami flos extract combined with Sappan lignum(The dosage: 20.0 g/kg crud drug of Carthami flos).Plasma samples were collected in heparinized tubes from the oculi chorioideae vein at 5,10,20,30,45,60,90,120,150,210,and 270 min after drug administration;and the plasma proteins were precipitated with 20% trichloroacetic acid aqueous solution.Plasma concentrations of HSYA were detected by RP-HPLC at different time points after drug administration.The data were processed by DAS 2.0 software to calculate the pharmacokinetic parameters.Results Compared with the Carthami flos group,the pharmacokinetic parameters V1/F and CL/F of HSYA in the Carthami flos combined with Sappan lignum group were significantly decreased(P0.01),and the AUC0-t,Cmax,and t1/2α of HSYA were significantly increased(P0.01).Conclusion Sappan lignum can promote the absorption of HSYA in rat model of CCBS and reduce the distribution of HSYA,thus exercise an efficacy-enhancing effect.

目的建立冠心七味片中丹参素、没食子酸、原儿茶酸和原儿茶醛测定方法。方法 Agilent TC-C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.2%甲酸水线性梯度洗脱,体积流量0.7 mL/min,检测波长275 nm,柱温25℃。结果丹参素、没食子酸、原儿茶酸和原儿茶醛分别在1.25~120μg/mL(r2=0.999 8)、8.75~350μg/mL(r2=1.000 0)、4.0~250μg/mL(r2=0.999 6)和2.5~375μg/mL(r2=0.999 9)质量浓度范围内具有良好的线性关系,方法回收率分别为99.5%、96.3%、94.8%和97.5%,最低检测限分别为9.0 ng/mL、2.5 ng/mL、7.5ng/mL和4.0 ng/mL。结论本方法操作快速简便,结果准确,重复性良好,可为冠心七味片的质量控制提供一定依据。

AIM To investigate the effects of Acorus tatarinowii Schott on pharmacokinetics of danshensu,protocatechuic aldehyde and protocatechuic acid in rats with compatibility of Salvia miltrorrhiza.METHODS Aqueous extracts of Salvia miltiorrhiza and Salvia miltiorrhiza plus Acorus tatarinowii Schott were orally administered to SD rats,respectively.The protein in the blood sample was precipitated by 10% trichloroacetic acid,and was extracted with ethyl acetate.Chromatogram of rats plasma was determined by RP-HPLC.RESULTS Both extract concentrations were all fitted with the two-compartment open model.In the compatibility,plasma drug concentration-time curves and absorption rate constant for danshensu and protocatechuic acid increased under the influence of Acorus tatarinowii Schott.CONCLUSION Acorus tatarinowii Schott could promote absorption of Salvia miltiorrhiza into the blood,and increase the bioavailability of danshensu and protocatechuic acid.