【目的】以落叶松胚性系为研究对象,建立和优化适合落叶松胚性愈伤组织增殖的悬浮培养体系,在此基础上对悬浮组织进行体细胞胚的成熟诱导。旨在为实现落叶松胚性愈伤组织的快速增殖及体细胞胚的规模化繁育奠定基础。【方法】对已诱导获得的长白落叶松、兴安×日本杂种落叶松及日本×长白杂种落叶松胚性愈伤组织进行继代培养,取新鲜增殖的组织进行悬浮培养。采用L9（34）正交试验设计,以胚性愈伤组织的增殖量及增殖率为响应值对悬浮培养条件进行筛选和优化,并对选出的最适培养条件进行验证。以悬浮增殖的胚性组织进行体细胞胚成熟培养,统计体胚发生量。【结果】在落叶松胚性愈伤组织的悬浮培养过程中,接种量、震荡强度及培养时间对胚性组织增殖具有显著的影响。在一定范围内,落叶松胚性组织的增殖量及增殖率随初始接种量的增加而降低,随震荡强度的升高呈先增加后下降,而随培养周期的延长而增加。以4 g·L -1 接种于含2,4-D 0.15 mg·L -1 、6-BA 0.05 mg·L -1 及KT 0.05 mg·L -1 的BM培养基（SCM）,在120 r·min -1 避光条件下悬浮培养,3个落叶松胚性系的胚性组织均能快速、稳定地增殖,培养15天的增殖率分别为2 569.42%、4 189.96%及3 001.67%,表现出较明显的种间差异。成熟培养方式对落叶松胚性悬浮组织的体胚发生量影响极显著（P=0.000）。悬浮培养获得的胚性组织接种到含琼脂6.0g·L -1 的固体增殖培养基（PCM）上继代15天后,转接到添加肌醇10 g·L -1 且无生长调节剂的1/4BM过渡培养基（TCM）上培养14天,再转入含有ABA 20 mg·L -1 、Ag NO35 mg·L -1 、PEG400080 g·L -1 的体胚成熟培养基上培养8周,体胚发生量显著提高（P=0.000）。在此条件下,3个落叶松胚性系OO-A1、GK-F1及KO-H的体胚发生量分别为（101.69±11.19）、（93.09±9.34）及（5.78±1.47）个·g -1 。【结论】悬浮培养能在短期内获得大量分散均匀、质量较高的落叶松胚性愈伤组织,且不会影响体细胞胚的发生和成熟。在BM液体培养基（SCM）中,接种量为4 g·L -1 、震荡强度为120 r·min -1 、暗培养15天,落叶松胚性组织的鲜质量可增加26.99~42.90倍。悬浮培养获得的胚性组织经固体增殖培养基（PCM）继代15天及过渡培养基（TCM）预培养14天后,再进行体胚成熟诱导可明显提高其体胚发生量。

采用正交试验设计,研究不同激素配比对鸡树条荚蒾茎段和叶片组培再生的影响,建立鸡树条荚蒾组培再生体系.结果表明:适于茎段分化的培养基为:WPM+2 mg/L 6-BA+0.2 mg/L IBA+20 g/L蔗糖,分化率为93.66％;适于叶片愈伤组织诱导的培养基为:WPM+2 mg/L 6-BA+0.1 mg/L IBA+2 mg/L 2,4-D,诱导率为93.33％,添加50 mg/L维生素C、50 mg/L活性炭可使叶片的褐化现象减轻;适于生根的培养基为:WPM+1.5 mg/L IBA+1 g/L活性炭,生根率为94.62％.

The objective of the study is to establish a rapid propagation system of hybrid blueberry .The wild blueberry of Dax-ing’ an Mountains and the cultivar of ‘Blomidon ’ were selected as parents to take intervarietal crosses and the hybrid seeds.The best sterile treatment conditions for hybrid seeds were selected and the sterile seedlings were got .The effects of the concentration of ZT , the contents of source and subculture cycle on frequency of cluster buds induction were conducted by using stem segments of seedlings as explants .The influence of different types of medium and concentration of IBA on the induction of adventitious root was analyzed .The contamination rate of hybrid seeds was 0 after sterilized with 75%ethanol and 3%sodium hypochlorite for 20 s and 7 min, respectively.The best medium for cluster buds induction was WPM contai-ning 0.6 mg· L-1 ZT and 20 g· L-1 source.The induction rate was up to 100%and the cluster buds grew well .During the bud induction, the optimal subculture cycle for buds proliferation was 30 d with the proliferation coefficient of 34.72.The best rooting medium was 1/2WPM containing 0.4 mg· L-1 IBA, the rooting rate was 88%, and the rooting period was only 40 d.The survival rate of transplanting was more than 85%.