Objective: This study aimed to elucidate the underlying mechanism of LncRNA PRKCA-AS1 in lung adenocarcinoma (LUAD). Methods: The expression of LncRNA PRKCA-AS1, miR-508-5p and S100A16, in LUAD tissues or cell lines (NCI-H520 and H1299) was analyzed with qRT-PCR. The clinical diagnostic value of LncRNA PRKCAAS1, miR-508-5p and S100A16 in LUAD were analyzed by receptor operating characteristic (ROC) curve. Then we knockdown or overexpression of PRKCAAS1 in NCI-H520 and H1299 cells, and the cell function test was applied to detect the activity and metastasis level of cells in different transfection groups. Then Pearson correlation analysis was used for the correlation between miR-508-5p and PRKCA-AS1. The dual-luciferase reporter experiment and CHIRP analysis was conducted to verify the target binding relationship of PRKCA-AS1, miR-508-5p or S100A16. FISH assay analyzed the colocalization of PRKCA-AS1 and miR-508-5p in NCI-H520 and H1299 cells. Rescue experiment and tumorigenesis experiment in nude mice further explore the regulatory mechanisms of LncRNA PRKCA-AS1, miR-508-5p and S100A16 on LUAD progression in vitro and in vivo. Results: From the results, PRKCA-AS1 and S100A16 were up-regulated in LUAD tissues, while miR-508-5p was downregulated compared with the adjacent tissues. And gain-of-function revealed that PRKCA-AS1 knock-down apparently suppressed the cell proliferation and metastasis, whereas miR-508-5p inhibitors or S100A16 overexpression showed a opposite effect. In addition, there is evidence that PRKCA-AS1, miR-508-5p and S100A16 have a targeted regulatory relationship. Moreover, rescue experiment and tumorigenesis experiment in nude mice further confirmed that LncRNA PRKCA-AS1 regulates S100A16 through sponging miR-508-5p to regulate LUAD progression in vitro and in vivo. Conclusion: These results demonstrated that LncRNA PRKCA-AS1 might regulate LUAD by acting as a ceRNA via sponging miR-508-5p and regulating S100A16 expression, indicating that manipulation of PRKCA-AS1 might be a potential therapeutic strategy in LUAD.

Objective: This study aimed to elucidate the underlying mechanism of LncRNA PRKCA-AS1 in lung adenocarcinoma (LUAD). Methods: The expression of LncRNA PRKCA-AS1, miR-508-5p and S100A16, in LUAD tissues or cell lines (NCI -H520 and H1299) was analyzed with qRT-PCR. The clinical diagnostic value of LncRNA PRKCAAS1, miR-508-5p and S100A16 in LUAD were analyzed by receptor operating characteristic (ROC) curve. Then we knockdown or overexpression of PRKCAAS1 in NCI -H520 and H1299 cells, and the cell function test was applied to detect the activity and metastasis level of cells in different transfection groups. Then Pearson correlation analysis was used for the correlation between miR-508-5p and PRKCA-AS1. The dual-luciferase reporter experiment and CHIRP analysis was conducted to verify the target binding relationship of PRKCA-AS1, miR-508-5p or S100A16. FISH assay analyzed the colocalization of PRKCA-AS1 and miR-508-5p in NCI -H520 and H1299 cells. Rescue experiment and tumorigenesis experiment in nude mice further explore the regulatory mechanisms of LncRNA PRKCA-AS1, miR-508-5p and S100A16 on LUAD progression in vitro and in vivo. Results: From the results, PRKCA-AS1 and S100A16 were up -regulated in LUAD tissues, while miR-508-5p was downregulated compared with the adjacent tissues. And gain -of -function revealed that PRKCA-AS1 knock -down apparently suppressed the cell proliferation and metastasis, whereas miR-508-5p inhibitors or S100A16 overexpression showed a opposite effect. In addition, there is evidence that PRKCA-AS1, miR-508-5p and S100A16 have a targeted regulatory relationship. Moreover, rescue experiment and tumorigenesis experiment in nude mice further confirmed that LncRNA PRKCA-AS1 regulates S100A16 through sponging miR-508-5p to regulate LUAD progression in vitro and in vivo. Conclusion: These results demonstrated that LncRNA PRKCA-AS1 might regulate LUAD by acting as a ceRNA via sponging miR-508-5p and regulating S100A16 expression, indicating that manipulation of PRKCA-AS1 might be a potential therapeutic strategy in LUAD.

2017年1月至2018年6月3例纵隔肿瘤伴上腔静脉阻塞综合征患者行全上腔静脉置换术，根据3D-CTBA重建影像技术进行术前手术规划，确定上腔静脉、左无名静脉、右无名静脉的形态、直径、受累范围和纵隔病灶的大小、部位。充分术前准备，麻醉干预。按照3D-CTBA重建结果，术中精准分离肿瘤周围组织，切除被侵组织肿瘤病灶和受累的上腔静脉、左无名静脉、右无名静脉部分。完整切除纵隔肿瘤，应用合适材料(人工血管、自体心包)行上腔静脉、左无名静脉、右无名静脉血管吻合重建。平均病灶直径7.5 cm，平均手术时间306 min，平均术中出血183 ml。术后病理诊断侵袭性胸腺瘤2例，胸腺癌1例。3例患者上腔静脉梗阻症状均得到改善，无围手术期死亡，随访至今均生存。

Background: The treatment of superior vena cava syndrome caused by invasive thymoma is challenging. This paper aims to explore the application of preoperative three-dimensional computed tomography bronchography and angiography (3D-CTBA) for total superior vena cava reconstruction. Methods: Total superior vena cava reconstruction guided by preoperative 3D-CTBA in the treatment of superior vena cava syndrome offers more accurate surgical evaluation and more effective procedure of multidisciplinary team (MDT), assists radical dissection and vascular reconstruction as planed in the way of "Step by Step". It also makes the follow-up procedure more effective. Results: High-quality thoracic computed tomography (CT) image is essential. A medical team ensures procedural success with 3D-CTBA. Using this approach, five patients have been treated successfully. The average operative length was 324 minutes and the average blood loss was 190 mL. There was no surgical mortality. Five patients are alive. Conclusions: Total superior vena cava reconstruction guided by preoperative 3D-CTBA is an effective technology for radical resection of mediastinal lesions combined with artificial vascular replacement. Meanwhile, 3D-CTBA improves the efficiency of MDT and surgical planning. It contributes to alleviate symptoms of SVCS and improve the quality of postoperative life.

INTRODUCTION:Oesophageal cancer is one of the most common malignant tumours and has been identified as one of the leading causes of cancer death worldwide. Surgery is considered to be the optimal treatment for patients with resectable oesophageal cancer. Oesophagectomy for oesophageal cancer can significantly extend the survival period of patients and provide a potential opportunity for a cure. However, there is still controversy regarding which thoracic approach (right or left) during oesophagectomy for oesophageal cancer can lead to better surgical outcomes globally. This systematic review and meta-analysis will be performed to determine which thoracic approach during oesophagectomy will achieve longer patient survival and will be more beneficial for patients.METHODS AND ANALYSIS:We will search PubMed, Web of Science, Embase, Cancerlit, the Cochrane Central Register of Controlled Trials and Google Scholar databases for relevant clinical trials published in any language before 1 October 2019. Randomised controlled trials (RCTs), quasi-RCTs, propensity score-matched comparative studies and prospective cohort studies of interest, published or unpublished, that meet the inclusion criteria will be included. Subgroup analysis of the type of operation, tumour pathological stage and ethnicity will be performed.PROSPERO REGISTRATION NUMBER:CRD42019124133.ETHICS AND DISSEMINATION:Because this study will be based on published or unpublished records and studies, there is no need for ethics approval. The results of the study will be published in a peer-reviewed journal.

Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed proliferation and invasion and promoted apoptosis in both EC cell lines. The sponging of miR-1294 by hsa_circ_0004370 was bioinformatically predicted and subsequently verified by luciferase reporter assay and RNA immunoprecipitation assay. Further, hsa_circ_0004370 involved in the up-regulation of LASP1 by sponging miR-1294. Besides, the inhibition of the down-regulated hsa_circ_0004370 on cell malignant behaviors was rescued by miR-1294 inhibitor. Finally, this rescue effect was abrogated by suppressing the expression of LASP1. The results present here suggest that hsa_circ_0004370 functions as an oncogene on cell proliferation, apoptosis, and invasion via miR-1294/LASP1 axis.

Objective To explore the protective mechanism of Heme Oxygenase-1 (HO-1) modified mesenchymal stem cells (MSC) in the treatment of lung transplantation ischemia-reperfusion injury (IRI).Methods Sixty SD rats were randomly divided into Surgical control (Ⅰ),simple MSCs (Ⅱ),empty vector-MSCs (Ⅲ),HO-1 modified MSCs (Ⅳ) were established.After establishing orthotopic left lung transplantation model in rats,group Ⅱ,Ⅲ and Ⅳ were injected with MSCs,empty vector-MSCs and HO-1-MSCs from pulmonary artery fine needles of recipient rats.Four hours later,partial pressure of oxygen (PaO2),partial pressure of carbon dioxide (PaCO2),tumor necrosis factor-alpha (TNF-α),Interleukin-10 (IL-10),wet/dry ratio (W/D),superoxide dismutase (SOD),malondialdehyde (MDA) and myeloperoxidase (MPO) were measured.Histopathological examination.Detection of HO-1-mRNA expression in transplants by reverse transcription polymerase chain reaction (RT-PCR).Multigroup mean comparison using One-way ANOVA.Results PaO2:Group Ⅰ,Ⅱ,Ⅲ and Ⅳ were (70.40 ±4.64),(87.53 ±4.01),(87.27 ±4.06),(96.80 ±647) mmHg (1 mmHg=0.133 kPa) gradually increased (F =75.578,P ＜ 0.01).Group Ⅱ and Ⅲ were similar (F =0.523,P ＞ 0.05).PaCO2:group Ⅰ,Ⅱ,Ⅲ and Ⅳ were (32.67 ±3.31),(27.80 ±2.98),(27.60 ±2.41),(19.87 ± 3.02) mmHg gradually decreased (F =48.286,P ＜ 0.01),group Ⅱ and Ⅲ were similar (F =0.480,P＞0.05).W/D:Group Ⅰ,Ⅱ,Ⅲ and Ⅳ were (4.96±0.22),(4.04±0.28),(4.11 ± 0.31),(3.85 ± 0.27) gradually decreased (F =48.992,P ＜ 0.01),group Ⅱ and Ⅲ were similar (F =1.819,P＞0.05).MDA content:In group Ⅰ,Ⅱ,Ⅲ and Ⅳ,the levels of MDA were (126.80 ± 11.14),(114.27 ± 16.45),(114.06 ± 17.68),(102.40 ± 14.21) nmol/g gradually decreased (F =6.551,P＜0.05),group Ⅱ and Ⅲ were similar (F=0.638,P＞0.05).MPO content:group Ⅰ,Ⅱ,Ⅲ and Ⅳ were (53.51 ±3.06),(45.07 ±3,32),(44.45 ±3.37),(40.60 ±2.47) U/g decreased gradually (F =46.907,P ＜ 0.01),group Ⅱ and Ⅲ were similar (F =1.772,P ＞ 0.05).SOD activity:In group Ⅰ,Ⅱ,Ⅲ and Ⅳ,SOD activity increased gradually [(304.60 ±34.74),(329.87 ± 19.44),(329.87±19.44),(338.73±15.77) U/g,(F=6.038,P＜0.01],while in group Ⅱ and Ⅲ,it was the same (F =0.727,P ＞ 0.05).TNF-α:In group Ⅰ,Ⅱ,Ⅲ and Ⅳ,the levels of TNF-αwere (61.56 ± 2.83),(48.32 ± 4.11),(48.53 ± 4.20),(36.77 ± 3.79) ng/L gradually decreased (F =108.215,P ＜ 0.01),group Ⅱ and group Ⅲ were similar (F =0.557,P ＞ 0.05).IL-10:The levels of IL-10 in group Ⅰ,Ⅱ,Ⅲ and Ⅳ were (23.43 ±2.37),(34.96 ±3.04),(35.48 ±3.00),(41.16 ± 3.12) ng/L gradually increased (F =98.751,P ＜ 0.01).In group Ⅱ and group Ⅲ,the levels were similar (F =0.437,P ＞ 0.05).Under light microscopy,neutrophils infiltrated pulmonary interstitium and inflammatory exudation was seen in alveolar cavity.Inflammation in group Ⅳ was the lightest in each group.The amplification bands of HO-1 mRNA in group Ⅳ were significantly stronger than other groups.Conclusion HO-1 gene modified MSCs have synergistic anti-injury effect in the treatment of lung transplantation IRI.

Abstract To investigate the effect of lung expansion and collapse method combined with closed vacuum aspiration technique on lung collapse time, reduce the waiting time of surgery. Forty patients with pulmonary peripheral nodules under thoracoscopic anatomical segmentectomy were divided into 20 cases of natural collapse group and 20 cases of modified collapse group. The natural collapse group used the traditional natural collapse method, and the modified collapse group used a lung expansion and collapse method combined with closed vacuum aspiration technique to record the lung collapse time and compare them. Thoracoscopic anatomical segmentectomy was successfully performed in both groups. The lung collapse time in the natural collapse group and the modified collapse group was 17.08 ± 1.35, 8.90 ± 0.39, respectively, P < .05. The lung expansion and collapse method combined with closed vacuum aspiration technique can reduced the waiting time of lung collapse during thoracoscopic anatomical segmentectomy, and can processed the inter-segment boundary better, thereby reduced the waiting time of surgery.