This study aims to assess the long-term effects of busulfan on male reproductive function in BALB/c mice to provide insight into its toxicological effects. A total of 80 BALB/c mice were randomly assigned to a control group (n = 20) and three experimental groups (n = 20 each), which received a single intraperitoneal injection of busulfan at 15, 20, and 25 mg/kg, respectively. A subset of mice was assessed at multiple time points for spermatogenesis. Additionally, fertility experiments with both sexes served as a vital measure of fertility potential. Results revealed that relative testicular weight and semen quality in the experimental groups were significantly decreased compared with those in the control group (p < 0.05). Abnormal sperm morphology showed a significant increase (p < 0.05), with a notable correlation observed between abnormal sperm neck morphology and reduced sperm motility (p < 0.05). Fertility trials indicated that busulfan-treated males had a reduced ability to impregnate females, as evidenced by reductions in pregnancy rates and litter sizes. Remarkably, the male offspring ratio in the busulfan-treated group (7 out of 17) was significantly lower than that in the control group (21 out of 42). In conclusion, a single intraperitoneal injection of busulfan led to sustained fertility impairment in mice for at least 13 weeks, with potential transgenerational effects, including alterations in the sex ratio of male descendants.

Inflammation is a key contributor to cardiovascular disease (CVD), yet existing risk models such as Atherosclerotic Cardiovascular Disease Risk in China (China-PAR) and Pooled Cohort Equations (PCE) inadequately identify individuals at intermediate risk. We investigated whether incorporating the inflammatory chemokine CCL17 could improve cardiovascular risk prediction. In two prospective cohorts—the Shunyi Cohort (n = 706, China) and the UK Biobank (n = 36,097, UK)—baseline CCL17 levels were quantified, and major adverse cardiovascular events (MACEs) were tracked over follow-up. Elevated CCL17 levels were independently associated with increased risk of MACEs. Integrating CCL17 into existing models significantly improved discrimination and reclassification, particularly among intermediate-risk individuals (e.g., NRI: 15.1

To compare efficacy and safety of glucagon-like peptide-1 receptor agonists (GLP-1RAs) in subjects with type 2 diabetes (T2DM). Electronic databases were searched from inception to 2nd October 2024 for randomised controlled trials comparing GLP-1RAs treating T2DM. Bayesian network meta-analyses were conducted to analyze metabolic and safety outcomes. 64 trials comprising of 25,572 participants were identified. Compared to placebo, tirzepatide showed the greatest reduction in HbA1-c (MD: -2.3%) and FPG (MD: -3.1mmol/L); semaglutide was second (HbA1-c: MD: -1.5%; FPG: MD: -2mmol/L); liraglutide was third (HbA1-c: MD: -1.2% FPG: MD: -1.6mmol/L) (P<0.05). All treatments showed no statistically significant differences in BMI, SBP, DBP, TC, HDL-C and LDL-C compared to placebo. Tirzepatide (MD: -9.1 kg), semaglutide (MD: -2.8 kg) and liraglutide (MD: -1.2 kg) (P<0.05) had significant reduction in body weight compared to placebo. GLP-1 RAs had higher risk of gastrointestinal symptoms. Semaglutide increased the risk of hypoglycemia compared to placebo while liraglutide reduced the risk of hypoglycemia compared to traditional antidiabetic drugs. GLP-1RAs improve glycaemic control, with tirzepatide, semaglutide and liraglutide exhibiting the most significant improvements. Tirzepatide is more suitable for treating T2DM with obesity. For individuals with normal weight, both semaglutide and liraglutide are generally more effective for treating T2DM. However, considering the potential for semaglutide to cause hypoglycemia, liraglutide may be the optimal choice for T2DM treatment to minimize the risk of hypoglycemia.

Per- and poly-fluoroalkyl substances (PFAS) have been reported to have hepatotoxic effects. However, it is unclear whether they are linked to non-alcoholic fatty liver disease (NAFLD). This nested case-control study focused on the epidemiological links between PFAS and the prevalence of NAFLD. We selected 476 new cases of NAFLD and 952 age- and sex-matched controls from the Jinchang cohort population between 2014 and 2019. Serum concentrations of PFAS were measured using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Only PFAS with a detection rate of ≥ 90 % were included for analysis, which included PFPeA, PFOA, PFNA, PFHxS, PFOS, and 9Cl-PF3ONS. The relationship between single and co-exposure to PFAS and the occurrence of NAFLD was evaluated using conditional logistic regression, Quantile g-computation (QgC), and Bayesian kernel machine regression (BKMR) model. Logistic regression indicated that PFPeA, PFOA, and 9Cl-PF3ONS were positive correlation with the incidence of NAFLD after adjusting for confounders, with odds ratios (OR) and 95 % confidence interval (CI) of 3.13 (95 % CI: 2.53, 3.86), 1.39 (95 % CI: 1.12, 1.73), and 1.41 (95 % CI: 1.20, 1.66), respectively. PFNA, PFHxS, and PFOS were nonlinearly and negatively associated with the incidence of NAFLD, with OR (95 % CI) of 0.53 (0.46, 0.62), 0.83 (0.73, 0.95), and 0.52 (0.44, 0.61), respectively. QgC showed a significant joint effect of PFAS on NAFLD onset (OR: 1.52, 95 % CI: 1.24, 1.88). BKMR showed a weak positive trend between PFAS mixtures and NAFLD incidence. Positive correlations were primarily driven by PFPeA and 9Cl-PF3ONS, while negative correlations were mainly influenced by PFNA and PFOS. The BKMR model also suggested that there was an interaction between PFOS and PFNA and other four PFAS compounds. In conclusion, our findings suggest that individual and co-exposure to PFAS is associated with a risk of NAFLD onset.

目的 通过对兰州市妇幼保健院24年新生儿死亡回顾分析,探讨新生儿死亡原因,为提出有效预防措施提供依据.方法 系统性收集该妇幼保健院1996-2019年新生儿死亡监测新生儿死亡情况,分析变化趋势及影响因素.结果 兰州市妇幼保健院新生儿死亡率呈逐年波动下降的趋势(x2=45.684,P＜0.01),从1996年的20.8‰下降至2019年的4.5‰,平均死亡率1.07％.新生儿死亡病因以新生儿窒息、先天畸形、低体质量、超低出生体质量为主;死亡新生儿中有明确围生期异常因素的占4.8％,其中常见的异常因素为脐带异常、羊水异常、前置胎盘、胎儿窘迫、妊娠期高血压等.出生缺陷在新生儿死亡中占比呈波动下降趋势.结论 加强孕产期、围生期保健、新生儿的早期监护和医护人员新生儿复苏技能的深化培训,提高新生儿救治水平,有效降低新生儿死亡率.

目的 用全外显子组学测序技术捕获和筛选新生儿先天性心脏病的基因突变.方法 于2014年1月—2018年4月,从兰州市妇幼保健院新生儿科明确诊断为以室缺伴房缺的严重先天性心脏病患儿中选取6例为研究对象,收集患儿与父母家系血样,通过全外显子测序技术捕获突变基因,采用公共数据库dbsnp、HapMap以及VEP和BGI内部数据库进行注释和筛选定位先天性心脏病的致病突变,用Sanger测序验证突变.结果 在6个家庭中有3个家庭发现了符合筛选条件的新生基因突变,其中3个基因发生错义突变,分别为FH2域控制蛋白1(FHDC1)(c.1977G>C;p.Gln659His)、溴区包含蛋白4(BRD4)(c.3797A>C;p.Glu1266Ala)和己糖激酶3(HK3)(c.397C>G;p.Leu133Val),一个基因剪切位点发生突变为sprouty相关EVH1域包含蛋白2(SPRED2)(c.373+1G>T).结论 FHDC1(c.1977G>C)、BRD4(c.3797A>C)、HK3(c.397C>G)、SPRED2(c.373+1G>T)可能为先天性心脏病有害的新生突变.

Modeling methods for busulfan-induced oligoasthenozoospermia are controversial. We aimed to systematically review the modeling method of busulfan-induced oligospermia and asthenozoospermia, and analyze changes in various evaluation indicators at different busulfan doses over time. We searched the Cochrane Library, PubMed databases, Web of Science, the Chinese National Knowledge Infrastructure, and the Chinese Biomedical Literature Service System until April 9, 2022. Animal experiments of busulfan-induced spermatogenesis dysfunction were included and screened. The model mortality and parameters of the evaluation indicators were subjected to meta-analysis. Twenty-nine animal studies were included (control/model: 669/1829). The mortality of mice increased with busulfan dose. Significant spermatogenesis impairment occurred within 5 weeks, regardless of busulfan dose (10–40 mg/kg). Testicular weight (weighted mean difference [WMD]: − 0.04, 95

Current studies on air pollutant exposure during pregnancy and orofacial clefts (OFCs) have inconsistent results, and few studies have investigated refined susceptible windows for OFCs. We aim to estimate association between air pollution and OFCs during the first trimester of pregnancy and identify specific susceptible windows. Birth data was obtained from Birth Defects Surveillance Network in Lanzhou from 2014 to 2019. Air pollution data and temperature data were obtained from ambient air monitoring stations and China Meteorological Data Network, respectively. A distribution lag nonlinear model (DLNM) was applied to estimate weekly-exposure-lag-response association between air pollutant levels and OFCs. The study included 320,787 perinatal infants from 2014 to 2019, of which 685 (2.14‰) were OFCs. The results demonstrated that exposure of pregnant women to aerodynamic diameter ≤ 10 μm (PM10) at lag 4–5 weeks was significantly associated with the risk of OFCs, with the greatest impact at the lag 4 week (RR = 1.029, 95% CI = 1.001–1.057). Exposure to sulfur dioxide (SO2) at lag 2–4 weeks was significantly associated with the risk of OFCs, with the greatest impact at the lag 3 week (RR = 1.096, 95% CI = 1.041–1.177). This study provides further evidence that exposure to air pollution increases the risk of OFCs in the first trimester of pregnancy.

The purpose of this systematic review is to evaluate the test accuracy of reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription-PCR (RT-PCR) for the diagnosis of coronavirus disease 2019 (COVID-19). We comprehensively searched PUBMED, Web of Science, the Cochrane Library, the Chinese National Knowledge Infrastructure, and the Chinese Biomedical Literature Service System until September 1, 2021. We included clinical studies assessing the sensitivity and specificity of RT-PCR and RT-LAMP using respiratory samples. Thirty-three studies were included with 9360 suspected cases of SARS-CoV-2 infection. The RT-PCR or other comprehensive diagnostic method was defined as the reference method. The results showed that the overall pooled sensitivity of RT-PCR and RT-LAMP was 0.96 (95 % CI, 0.93 0.98) and 0.92 (95 % CI, 0.85 0.96), respectively. RT-PCR and RT-LAMP had a 0.06 (95 % CI, 0.04 0.08) and 0.12 (95 % CI, 0.06 0.16) false-negative rates (FNR), respectively. Moreover, subgroup analysis showed mixed sampling and multiple target gene diagnosis methods had better diagnostic value than single-site sampling and a single target gene. The sensitivity and FNR were also significantly affected by the reference method. Comparing RT-LAMP with established suboptimal RT-PCR may exaggerate the performance of RT-LAMP. RT-PCR and RT-LAMP showed high values in the diagnosis of COVID-19, but there was still a FNR of about 6%-12%.

Abstract Background: There are many traditional Chinese medicine prescriptions for the treatment of type 2 diabetes (T2DM), but most of them are not simple enough, which increase the economic burden of patients. Radix Astragali, Radix Puerariae, Radix Trichosanthis and Radix Rehmanniaeare the four traditional Chinese medicines commonly used in the treatment of T2DM. However, the molecular mechanism of these four drugs in the treatment of diabetes is still unclear. Therefore, this study is the first to explore the potential mechanism of Astragali-Rehmanniaeare and Puerariae-Trichosanthis in the treatment of T2DM through network pharmacology and animal experiments.Methods: First we obtained the active chemical components and targets of these four drugs. Then the main targets of diabetes were obtained and protein-protein interaction was built by String. Metascape platform was used to analyze the "drug-component-target" and the biological processes and pathways they involved. Finally, "Drug-Diabetes-Pathway" network was conducted. Subsequently, animal experiments were conducted to verify the network analysis results. Blood glucose of two hours postprandial was measured every week. The insulin expression level was measured to calculate HOMA-IR and HOMA-β, and the protein expressions of PI3K and Akt were measured as well.Results: The core active components were quercetin, daidzein, kaempferol, puerarin, formononetin; the core targets includedAKT1, PIK3CA, TNF, etc. The biological pathway mainly acted on PI3K-Akt signaling pathway and insulin resistance pathway. The experiment results showed that the drug groups could significantly reduce the blood glucose of T2DM rats. HOMA-IR of Astragali-Rehmanniaeare was significantly decreased, and HOMA-β of Puerariae-Trichosanthis was significantly increased. PI3K protein in Astragali-Rehmanniaeare and Puerariae-Trichosanthis was significantly higher than that in control group. Akt protein in Astragali-Rehmanniaeare was significantly higher than that in control group, but significantly lower than that in model group.Conclusions: Astragali-Rehmanniaeare and Puerariae-Trichosanthis improved blood glucose mainly by changing the contents of PI3K and Akt in the body to affect the PI3K-Akt signaling pathway, so as to achieve the purpose of treating T2DM.

Exposure to air pollution during pregnancy has been linked to birth defects. But the directions of studies on the associations between air pollutants exposure and effect on the incidence of congenital heart disease (CHDs) were inconsistent. To date, few studies were concentrated on the effects of both particulate matter and gaseous air pollutant exposure on CHDs across the full gestational week simultaneously. Our study aimed to investigate the critical exposure windows for each air pollutant throughout 40 gestational weeks. Data on CHDs, air pollution, and meteorological factors from 2013 to 2019 were collected in Lanzhou, China. A distributed lag nonlinear model combined with a quasi-Poisson regression model was applied to evaluate the weekly exposure–lag–response association between air pollutants levels and CHDs, and the subgroup analyses were conducted by gender (baby boy and baby girl). The study included 1607 mother–infant pairs. The results demonstrated that exposure of pregnant women to particulate matter ≤ 5 μm (PM2.5) at lag 1–4 weeks was significantly associated with the risk of CHDs, and the strongest effects were observed in the lag 1 week (1.150, 95

Few studies have evaluated the association between air pollutants and neural tube defects (NTDs). Moreover, the existing research ignores the lag effect of air pollution on health and provides inconsistent epidemiological evidence. We aim to estimate the association between air pollution and NTDs during the first trimester of pregnancy and identify specific susceptible windows. Birth data was collected from the Birth Defects Surveillance Network in Lanzhou from September 1, 2014, to December 31, 2019. Air quality and meteorological data were collected from ambient air monitoring stations and China Meteorological Data Network. The log connection function of the Poisson distribution function is used to establish a DLNM model to estimate the exposure–effect relationship and exposure–lag relationship association between air pollutants levels and NTDs. There were 320,787 perinatal infants in Lanzhou from September 1, 2014, to December 31, 2019, and 486 cases of NTDs (1.5‰). The result indicates that exposure to inhalable particles (PM10) at lag 2–4 weeks was significantly associated with the risk of NTDs, with the most significant impact at the lag 2 week (RR=1.048, 95

目的 应用全外显子组学测序技术筛选先天性心脏病患儿基因突变位点,并分析这些位点所涉及的通路.方法 提取6例均有室缺伴房缺的严重先天性心脏病患儿基因组DNA,通过全外显子组芯片捕获技术发现突变并筛选出与CHD有关的共有突变,并进一步进行功能GO富集分析.结果 6例患儿中有27个SNP位点涉及17个基因,46个InDel位点涉及33个基因是符合筛选条件的共有突变,经过功能GO富集分析,SNP和InDel突变基因富集到poly(A)结合(GO:0008143)、调节心脏收缩(GO:0008016)、锌离子结合通路(GO:0008270)、机械刺激感受通路(GO:0050982)、泛素依赖的蛋白质分解代谢过程(GO:0042787)、钙离子结合通路(GO:0005509),这6条通路具有统计学意义.结论 全外显子组芯片捕获技术是筛选CHD发生突变基因和功能分析的有效方法.

目的 根据2010-2019年兰州市婚前检查率,探讨兰州市婚前检查率变化趋势对兰州市出生缺陷监测报告检出率的影响.方法 对2010-2019年兰州市出生缺陷监测数据进行整理分析,采用线性趋势卡方检验分析兰州市出生缺陷检出率及婚前检查率的时间趋势,Spearman法、一元线性回归分析婚前检查率与出生缺陷检出率的关系.结果 近10年兰州市出生缺陷检出率呈逐年上升趋势(x2=504.274,P＜0.001),从2010年82.61/万上升至2019年的221.61/万,增幅达到了168.26％.婚前检查率与出生缺陷检出率二者呈负相关关系,相关系数为-0.976.一元线性回归模型结果显示,婚前检查率每提高1％,出生缺陷检出率降低1.275/万.结论 婚检是优生优育、预防出生缺陷的第一道防线,做好婚检工作对降低出生缺陷具有重要意义.

Aim: A stable induced type 2 diabetes model (T2DM) still needs to be explored for basic and clinical research, due to nonuniform model methods and unstable consequences. Our aims were to explore and establish an optimized induced T2DM model in mice that exhibits insulin resistance and beta-cell damage. Materials and methods: C57BL/6 mice were treated with a high-fat diet (HFD), streptozotocin (STZ) and dexamethasone (DEX) at different doses and in combination. The general growth status, blood glucose and fasting insulin were detected, and the success rate and insulin sensitivity indices were calculated. Key finding: Low-dose STZ injection multiple times was more secure in the process of T2DM model production. Combined intervention was more efficient in reducing insulin sensitivity and improving the success rate of T2DM model construction. Significance: Combined with a high-fat diet, glucocorticoids and streptozotocin, a new mouse model of T2DM with insulin resistance and beta-cell damage could be established. The optimized experimental method can serve as a stable model for further studies on the mechanisms and therapy of T2DM.

Objective: Some studies have shown that metformin can reduce body weight. However, metformin has not been officially approved as a medicine for weight loss because its effect on different populations remains inconsistent. This meta-analysis aimed to summarize the weight loss effect of metformin quantitatively. Method: The randomized controlled and high-quality case-control trials of metformin monotherapy in obesity treatment were eligible. Baseline body mass index (BMI) was chosen as a self-control to compare the changes in BMI of different populations before and after treatment. All changes were calculated as differences between the final and initial BMI values (with negative values indicating a decrease). Results were presented as weighted mean difference (WMD) with a 95% confidence interval (CI 95%). Subgroup analysis was performed based on baseline BMI, age, daily dose, and duration of medication. Results: A total of 21 trials ( n = 1004) were included, and the meta-analysis of metformin treatment in different populations showed that metformin has a modest reduction in the BMI of included participants (WMD −0.98; 95% CI, −1.25 to −0.72), and the reduction of BMI was most significant in the simple obesity population (WMD −1.31; 95% CI, −2.07 to −0.54). The subgroup analysis showed that metformin treatment significantly reduced BMI in obesity patients with a BMI >35kg/m2 (WMD −1.12; 95% CI, −1.84 to −0.39) compared with before treatment. BMI in the high dose group decreased by 1.01 units (WMD−1.01; 95% CI, −1.29 to −0.73) and BMI did not continue to decrease significantly after treatment of more than 6 months. Conclusion: Patients treated with metformin experienced about a one-unit reduction in BMI at the end of treatment. But whether this decreased value produced enough weight loss (5% of baseline body weight) to qualify as a “weight loss drug” as current guidelines require, requires larger specific randomized control trials.

Introduction and objectives: The purpose of this study was to confirm whether hepatitis B virus (HBV) infection and the levels of liver enzymes would increase the risk of prediabetes and diabetes mellitus (DM) in China. Materials and methods: A total of 10,741 individuals was enrolled in this prospective cohort study. Cox regression analysis was used to calculate the Hazard ratios (HRs) to evaluate the relationships between HBV infection and the risk of DM and prediabetes. Decision trees and dose response analysis were used to explore the effects of liver enzymes levels on DM and prediabetes. Results: In baseline population, HBV infection ratio was 5.31%. In non-adjustment model, the HR of DM in HBV infection group was 1.312 (95% CI, 0.529-3.254). In model adjusted for gender, age and liver cirrhosis, the HR of DM in HBV infection group were 1.188 (95% CI, 0.478-2.951). In model adjusted for gender, age, liver cirrhosis, smoking, drinking, the HR of DM was 1.178 (95% CI, 0.473-2.934). In model further adjusted for education, family income and occupation, the HR of DM was 1.230 (95% CI, 0.493-3.067). With the increases of levels of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Gamma-glutamyl transferase (GGT), the risk of prediabetes was gradually increasing (Pnon-linearity < 0.05). There were dose-response relationships between ALT, GGT and the risk of DM (Pnon-linearity < 0.05). Conclusions: HBV infection was not associated with the risk of prediabetes and DM. The levels of liver enzymes increased the risk of prediabetes and DM. (C) 2019 Fundacion Clinica Medica Sur, A.C. Published by Elsevier Espana, S.L.U.

BACKGROUND:Some studies found out that TC/HDL-C ratio is a predictor of Cardiovascular disease (CVD) and Nonalcoholic fatty liver (NAFLD) is related to CVD. And some researches have already studied that Apolipoprotein B to Apolipoprotein A1 ratio (ApoB/ApoA1) and Triglyceride to High-density lipoprotein cholesterol ratio (TG/HDL-C) were both related with CVD and NAFLD, but few studied the association between TC/HDL-C ratio and NAFLD. So, we suspected the ratio was also related to NAFLD. The research aims to study the predictive value of TC/HDL-C to NAFLD and to help the early detection of NAFLD.METHODS:Based on the Jinchang Cohort, the study contained 32,121 participants. We assessed the incidence of NAFLD by the quartiles of TC, HDL-C and TC/HDL-C. Then, the does-response relationship between these indicators and the risk of NAFLD was obtained. Finally, the receiver operator characteristic curve (ROC) was applied to decide the predictive value of TC/HDL-C.RESULTS:Among the study participants, the cumulative incidence of NAFLD was 6.30% and the rate of dyslipidemia was 40.37%. The biochemical indicators of NAFLD had a difference with general population. The incidence of NAFLD raised with the quartiles of TC, TG and LDL-C raising, while decreased with the HDL-C' quartiles raising. After controlling confounding factors, TC and TC/HD-C had a positive relationship with NAFLD, while HDL-C had the opposite. Finally, the ROC analysis showed the area under the curve (AUC) of TC/HDL-C (0.645) was greater than TC (0.554), HDL-C (0.627) and Apolipoprotein B to Apolipoprotein A1 (ApoB/ApoA1) (0.613).CONCLUSIONS:The TC/HDL-C ratio has significant predictive value to NAFLD.

Background: Occupational nickel exposure can cause DNA oxidative damage and influence DNA repair. However, the underlying mechanism of nickel-induced high-risk of lung cancer has not been fully understood. Our study aims to evaluate whether the nickel-induced oxidative damage and DNA repair were correlated with the alterations in Smad2 phosphorylation status and Nkx2.1 expression levels, which has been considered as the lung cancer initiation gene. Methods: 140 nickel smelters and 140 age-matched administrative officers were randomly stratified by service length from Jinchang Cohort. Canonical regression, χ2 test, Spearman correlation etc. were used to evaluate the association among service length, MDA, 8-OHdG, hOGG1, PARP, pSmad2, and Nkx2.1. Results: The concentrations of MDA, PARP, pSmad2, and Nkx2.1 significantly increased. Nkx2.1 (rs = 0.312, p < 0.001) and Smad2 phosphorylation levels (rs = 0.232, p = 0.006) were positively correlated with the employment length in nickel smelters, which was not observed in the administrative officer group. Also, elevation of Nkx2.1 expression was positively correlated with service length, 8-OHdG, PARP, hOGG1 and pSmad2 levels in nickel smelters. Conclusions: Occupational nickel exposure could increase the expression of Nkx2.1 and pSmad2, which correlated with the nickel-induced oxidative damage and DNA repair change.

Objective: To understand the incidence of stroke in the population of Jinchang Cohort and the relationship between metabolic diseases and stroke, and provide scientific evidence for the prevention and treatment of stroke in the population. Methods: The epidemiological investigation data and physical examination data of the 33 042 follow-up participants in Jinchang Cohort were collected for a prospective cohort study. Restricted cubic splines functions was used to analyze the dose-response relationship between metabolic indexes and the risk of stroke incidence. Results: 1) The incidence rate of stroke in Jinchang Cohort was 1.59%, and the standardized incidence rate was 3.99%. 2) Hypertension (male HR=2.20, female HR=4.45) and dyslipidemia (male HR=1.49, female HR=1.79) were the risk factors of stroke incidence in the population and diabetes had influence on the incidence of stroke only in the males (HR=1.79), while obesity had influence only in the females (HR=1.64). The more kinds of metabolic diseases, the higher risk of stroke incidence was. 3) Systolic blood pressure had a non-linear dose-response correlation with the risk of stroke incidence, while diastolic blood pressure had a positive linear correlation with the risk of stroke incidence. Conclusions: The incidence of stroke in Jinchang Cohort population was high compared with both domestic level and oversea level. The patients with metabolic diseases were the population at high-risk for stroke, and more attention should be paid to them in the prevention and treatment of stroke. Diastolic blood pressure might be more closely related to stroke.