Objective:To investigate the relationship between the expression of tumor-infiltrating lymphocytes (TILs) and clinicopathological characteristics, and the effect of TILs on prognosis in patients with human epidermal growth factor receptor 2 (HER2) positive breast cancer.Methods:A total of 94 female patients with HER2 positive breast cancer treated at Tengzhou Central People's Hospital from October 2016 to October 2018 were collected, with a median age of 51 years (32-73 years). Among them, 49 patients took trastuzumab and chemotherapy, and 45 chemotherapy. The degree of TILs in the tumor area of each patient's HE section was evaluated. χ2 test was used to analyze the category data. The Kaplan Meier survival curve and Cox proportional hazard regression model were used to analyze the prognostic factors. The relationship between the degree of TILs and the clinicopathological characteristics and the effect of TILs on the patients' recurrence free survival (RFS) were analyzed. Results:Among the 94 patients, 61 had low infiltration, and 33 high infiltration. There were no significant correlations between the degree of TILs and clinical pathological parameters (all P>0.05). In the targeted therapy group, 32 cases had low infiltration, and 17 cases high infiltration. The RFS of the patients with high TILs was longer than that of the patients with with low TILs ( P=0.036); TNM stage and the degree of TILs were independent factors for the patients' prognosis ( P=0.003 and 0.029). In the general chemotherapy group, 29 cases had low infiltration, and 16 cases high infiltration. The RFS of the patients with high TILs was longer than that of the patients with with low TILs ( P=0.049); TNM stage and TILs were independent factors related to the patients' prognosis ( P=0.001 and 0.028). Conclusions:TILs can be used as tumor markers in predicting the prognosis and therapeutic effect of patients with HER2 positive breast cancer, and may become new therapeutic targets for tumors.

Objective:To investigate the relationship between DSG3 and Ki-67 expressions in patients with non-small cell lung cancer (NSCLC) and its effect on prognosis.Methods:A total of 72 patients with NSCLC, which were surgically removed and diagnosed by routine pathological section examination in Tengzhou Central People's Hospital from July 2015 to December 2019, were collected. The expressions of DSG3 and Ki-67 in these patients were detected by immunohistochemistry, and the relationship between DSG3 expression and Ki-67 proliferation index was analyzed. The clinicopathologic factors associated with tumor prognosis, including age, gender, tumor diameter, pathological type, tumor differentiation, and lymph node metastasis, were collected. They were followed up regularly. The relationship between DSG3 expression and these clinicopathological data was statistically analyzed. Kaplan-Meier method was used to evaluate the effect of DSG3 expression on the patients' prognosis.Results:Among the 72 patients, there were 51 males and 21 females, with a median age of 65. Thirty patients had lung squamous cell carcinoma, and 42 had lung adenocarcinoma. Twenty-nine patients were positive in DSG3, and 43 negative. The Ki-67 proliferation index was (35.00±14.02)% in the lung squamous cell carcinoma group, and was (28.81±13.83)% in the lung adenocarcinoma group ( P=0.067). The Ki-67 proliferation index was (27.07±12.21)% in the DSG3-positive group, and was (34.30±14.74)% in the DSG3-negative group ( P=0.032). The expression of DSG3 was correlated with pathological type, tumor differentiation status, and lymph node metastasis ( χ 2=33.734, P＜0.001; χ 2=6.847, P=0.033; χ2=5.855, P=0.016), but not with other clinicopathologic features (all P＞0.05). Kaplan-Meier analysis showed that there was no statistical difference in the total survival time between the DSG3 positive and negative groups ( P=0.204). Conclusion:The expression of DSG3 in NSCLC is correlated with Ki-67 proliferation index, and has some prognostic value in NSCLC patients.

Objective To detect the expression and the differential significance of CK 5／6,DSG3,P40,TTF-1,CK7,NapsinA in small biopsy specimens of non -small cell lung cancer ( NSCLC),squamous cell carcinoma (SCC) and adenocarcinoma (AC).Methods Immunohistochemical SP method was used to detect the expressions of CK5／6,DSG3,P40,TTF-1,CK7 and NapsinA in 120 small biopsy specimens of NSCLC hospitalized in the Central People's Hospital of Tengzhou from January 2016 to December 2017,and the results were analyzed combined with the clinical characteristics of NSCLC.Results The positive expression rates of CK5／6,DSG3 and P40 in lung SCC were 100.0%(56／56),89.3%(50／56) and 96.4%(54／56), respectively,with specificity of 90.6%,100.0% and 100.0%,respectively.The positive expression rates of NapsinA ,TTF-1 and CK7 in lung AC were 81.3%(52／64), 90.6%(58／64) and 93.8%(60／64),respectively,with specificity of 100.0%,92.9% and 96.4%,respectively. The positive expression rates of CK5／6,DSG3,P40 in SCC had statistically significant differences compared with those in AC (all P＜0.05),and the expression of TTF -1,CK7 and NapsinA in AC had statistically significant differences compared with those in SCC ( all P＜0.05).Conclusion CK5／6,DSG3,P40,TTF-1,CK7 and NapsinA are of great significance in the differential diagnosis of SCC and AC in small biopsy specimens of NSCLC .