目的探讨激活的NF-κB及调节活化正常T细胞表达和趋化因子（RANTES）的表达及其在升主动脉瘤形成中的作用。方法4周龄雌性Wistar大鼠40只,随机分成升主动脉瘤组（20只）和正常对照组（20只）。制作升主动脉瘤动物模型,术后3个月取材,免疫组织化学方法分析NF-κB、RANTES的蛋白表达,RT-PCR方法检测其mRNA的表达。结果模型组动脉壁NF-κB和RANTES表达明显升高,而正常动脉壁中未见或极少见NF-κB和RANTES表达。RT-PCR显示,模型组的动脉瘤内NF-κB和RANTES mRNA表达增强,两者呈显著性正相关。结论NF-κB和RANTES在动脉瘤中表达强于对照组,NF-κB和RANTES在升主动脉瘤病变的发生发展过程中可能起重要作用。
Objective To investigate the activity changes of gelatinase in the formation of ascending aortic aneurysm.Methods Thirty five young Wistar rats were divided into two groups:the control group and the experiment group.The rat models induced by ascending aorta banding were made.The ascending aortas were taken after 3-5 months operation,changes of gelatinase activity was observed by gelatin zymography and film in situ zymography.Results Gelatinase activity of ascending aortic aneurysm was significantly increased compared with that of normal ascending aortic aorta.Conclusion Elevation of gelatinase activity may play a significant role in the formation of ascending aortic aneurysm.
Objective To investigate the expressions and significance of transforming growth factor-beta 1(TGFβ1) and its typeⅡ receptor(TGFβRⅡ) in experimental rat ascending aortic aneurysm of rat model.Methods The rat ascending aortic aneurysm models were made by banding ascending aorta of young Wistar rats.The ascending aortas were taken 4 months after operation.Immunohistochemistry staining and Western blotting were used to investigate the expressions of TGFβ1 and TGFβRⅡ.Result Immunohistochemistry staining results showed that TGFβ1 expressed in all layers of the aortic aneurysm and the control.TGFβRⅡ was extensively located in the hyperplastic intima and tunica media smooth muscle cells in the aortic aneurysm,while there was only a little positive staining in the control group.Western blotting results indicated that the expression levels of TGFβ1 and TGFβRⅡ in the aortic aneurysm were stronger than the control,P0.05 and P0.01 respectively.Conclusion The expressions of TGFβ1 and TGFβRⅡ in ascending aortic aneurysm are stronger than that in the control.The TGFβ signaling may contribute to the development of aortic aneurysm.
