Objective To investigate the correlation between histone deacetylase 9 (HDAC9) gene susceptible single nucleotide polymorphisms (SNPs) and genotype and its phenotype in large vessel atherosclerosis-induced ischemic stroke. <br> Methods By using the Haploview software and data on genetics from thousands of human genome project (HapMap) , the analysis of SNPs of HDAC9 gene, minor allele frequency (MAF), single block and haplotype and tag SNPs were performed. <br> Results A total of 75 SNPs in the Chr7:18930kb--19020kb regions of HDAC9 gene were determined, of which 51 SNPs accorded with Hardy-Weinberg balance (P>0.05), and MAF (P>0.05). Based on the conifdence intervals, four Gamete rule and solid spine of LD, the 11, 14 and 8 single domains were constructed, respectively. Among which, the rs2717356 site was low linkage disequilibrium (LD) and outside of the single domain belonging to HDAC9 gene recombination hotspots. A total of 30 SNPs were determined as haplotype tagging SNPs (htSNPs) according with R2 was more than or equal to 0.8, and LOD was more than or equal to 3.0, and that showed MAF was more than 0.10. The rs2526630 [C/T] site located in HDAC9 3 'UTR region, binding with microRNAs like hsa-miR-545, hsa-miR-616, etc. <br> Conclusion The 30 htSNPs served as reference for HDAC9 gene susceptibility SNP sites for screening and validating repeatedly. The binding of rs2526630 site of HDAC9 gene and miRNA might associate with the morbidity of large vessel atherosclerosis-induced ischemic stroke.

目的 观察前列宁胶囊对良性前列腺增生模型大鼠前列腺组织中EGF、EGFR表达的影响.方法 将雄性SD大鼠60只,随机分成空白组、模型组、保列治组、前列宁胶囊(低、中、高剂量)组;去势后皮下注射丙酸睾酮复制大鼠前列腺增生模型,连续给药28 d,观察各组前列腺涅重及指数,RT-PCR检测大鼠前列腺组织中EGF、EGFR的mRNA表达,免疫组化法观察前列腺组织EGF及EGFR的蛋白表达情况.结果 模型组前列腺湿重、指数明显增加,和正常组相比有显著差异性(P<0.01),前列腺组织EGF及EGFR的mRNA和蛋白表达较正常组均显著升高(P<0.01);和模型组相比,前列宁胶囊各剂量组和保列治组的前列腺湿重与指数明显降低(P<0.05),前列腺组织EGF及EGFR的mRNA和蛋白表达与模型组相比均显著降低(P<0.05).结论 前列宁胶囊对BPH大鼠有明显的治疗作用,能显著地抑制BPH大鼠EGF及EGFR的表达,提示这可能是前列宁胶囊治疗BPH的重要机制之一.