2018年4月至2018年10月在江苏省无锡宜兴、常州武进、溧阳和金坛、淮安宝应、宿迁泗洪等6个市(区、县)149个患细菌性败血症的团头鲂养殖场,无菌采集样品肝脏、脾脏、肾脏、腹水进行细菌分离纯化.分离纯化后进行生化鉴定,生化鉴定为嗜水气单胞菌的菌株再用16 S rRNA-PCR进一步鉴定.结果显示,经过分离纯化得到149株细菌,生化鉴定117株为嗜水气单胞菌,16S rRNA-PCR进行验证核对,得到101株嗜水气单胞菌,其余16株可以鉴定到气单胞菌属.对采样数据进行综合分析,在6个采样地区中,嗜水气单胞菌阳性率最低为金坛的57.14％,最高为宝应的75％.在不同规格样品中,0.2 kg～0.3 kg的团头鲂嗜水气单胞菌阳性率最高(76.12％).结果表明,江苏人工养殖团头鲂细菌性败血症的主要病原为嗜水气单胞菌,对其进行防控刻不容缓.

Objective To evaluate the genotoxicity of tenofovir dipivoxil fumarate (TDF) and provide the theory basis for its clinical medication. Methods Salmonella reversion test (Ames test), chromosomal aberration test of CHL cells and mouse marrow micronucleus assay were used to investigate the genotoxicity of TDF. Results Various dosages of TDF showed no mutagenic effects on salmonel latyphimurium in the Ames test, no chromosomal aberration effects were observed after CHL cells exposed to various dosages of TDF with or without rat liver supernatant 9 (S9) and TDF did not have inductive effects on formation of polychromatic erythrocyte micronucleus in mouse bone marrow micronucleus test. Conclusion TDF has no genotoxicity based on the Ames test, CHL chromosomal aberration test and micronucleus assay in the experimental conditions.