Objective:To observe distribution and number of T cells in human normal cervical tissue and provide experimental data for further studies like expressions of T cells in cervical lesions and cervical cancer tissues. Methods:The number, distribution and expression intensity of the surface differentiation antigen CD3, CD4, CD8 positive T cells in the normal cervical tissue were detec-ted by immunohistochemical SP technique and computerized imaging analysis system. Results:The CD3 positive cells mainly distribu-ted in the cervical mucosal epithelium and connective tissue above the adjacent lamina propria. The CD8 positive cells widely distribu-ted in the human cervical mucosal lamina propria connective tissue. The CD4 positive cells mainly distributed in the lamina propria connective tissue under the human cervical epithelium and the connective tissue around the cervical mucosa adenoid fossae. The num-ber of CD4 positive cells was higher than that of CD8 positive cells. Conclusions: In the human normal cervical tissue, The CD3, CD4, CD8 positive cells maintain a certain proportions.