目的比较类风湿关节炎(rheumatoid arthritis,RA)、骨关节炎(osteo arthritisk,OA)和创伤后手术(post-traumatic,PT)患者滑膜细胞产生巨噬细胞炎症蛋白1-α(MIP-1α)、MIP-1β和活化正常T细胞表达和分泌调节因子(RANTES)的差异,探讨MIP-1α在体外能否诱导RA滑膜成纤维细胞增殖反应.方法收集RA、OA和PT患者滑膜组织.采用胶原酶消化法获得滑膜细胞,采用组织块贴壁法获得滑膜成纤维细胞.滑膜细胞培养上清中MIP-1α、MIP-1β和RANTES检测采用ELISA法.滑膜成纤维细胞增殖反应采用四甲基偶氮唑盐微量酶反应比色法(MTT法).结果 RA、OA和PT患者的滑膜细胞在体外不能自发产生MIP-1α、MIP-1β和RANTES.用LPS(5μg/ml)和白介素-1α(rhIL-1α,50U/ml)刺激滑膜细胞后,RA患者滑膜细胞可产生较高的MIP-1α和RANTES,并明显多于OA和PT患者滑膜细胞产生的量,而滑膜细胞产生MIP-1β量在3种类型患者中无明显差异.MIP-1α可诱导RA滑膜成纤维细胞增殖反应,并且在一定浓度范围内(2～50ng/ml)具有浓度依赖性.结论RA滑膜细胞在体外产生MIP-1α和RANTES的量明显高于OA和PT患者,MIP-1α诱导RA滑膜成纤维细胞的增殖反应.提示MIP-1α在RA的发病机制中可能起重要作用.

Objective: The study was to assess the feasibility of gene transfer to homograft conduits after cryopreservation. Methods: The New Zealand white rabbits (adult n=15, infant n=15) were divided into five groups. Each group included three recipients(adult) and three donors(infant). Six homograft conduits (three aortic and three pulmonary) were havested from donors and then were stored in liquid nitrogen. Homograft conduits in five different groups were cryopreserved for 7、14、30、90 and 180 days, respectively. After being thawed quickly at temperature 37～42℃, 5 grafts of each group were incubated in 300 μl DMEM containing adenovirus carry the β-galactosidas gene, one of each group was incubated 300 μl DMEM which did not contain adenovirus. After 30 minutes incubation, bilateral aorto-cartid transplantation were performed in 3 recipient rabbits. The homografts were harvested at 5 days after transplantation and assayed for β-galactosidase activity. Results: Successful gene transfer and expression of the β-gal gene in all homograft conduits. Control group showed no transduction. No significant difference among the treated groups(P＞0.05). Conclusion: It is feasible to perform adenoviral-mediated gene transfer to homograft conduits after cryopreservation.