Objective [WT5”BZ]To observe the enzymic histochemical and ultrastructral changes of cryopreserved human retina. [WT5”HZ]Methods [WT5”BZ]To compare the activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and ATPase in cryopreserved retina with those in fresh retina and to observe the histological and ultrastructural changes of cryopreserved retina. [WT5”HZ]Results [WT5”BZ]There was no statistical difference between the activity of LDH, SDH and ATPase in fresh and in cryopreserved retina. Histologically, in the cryopreserved retina, fluid in neural fiber and outer plexiform layers, as well as in cone and rod layer, was sligthly more than normal. The ultrastructure is normal except that the mitochondria was swollen in different degree. [WT5”HZ]Conclusion [WT5”BZ]Cryopreservation may be an effective method for keeping the retinal cells alive for a long period and might free the transplantation from dependance on aviability of fresh dornor tissue. [WT5”HZ]

自1959年Royo等报道了第1例视网膜移植以来,视网膜移植经历了前房内移植、玻璃体腔移植、中枢神经系统移植.目前多集中于最接近于其生理功能的视网膜内及视网膜下移植的研究.虽然这项技术还没有达到人们所期望的程度,但随着分子生物学、遗传学等的发展及显微手术技巧的提高,视网膜移植术已展现出诱人的前景.本文就视网膜移植有关的几个主要问题及其研究现状作一综述.