对致病菌—尖孢镰刀菌(Fusrium oxysporum)具有较强拮抗作用的枯草芽孢杆菌(Bacillus subtilis)8-32产生的理化性质进行了初步研究.结果表明,该抗菌物质80℃处理30 min,拮抗活性仍保持在90.00％,100℃以上会完全丧失活性;在pH 7.0～11.0范围内抑菌活性较稳定,最适pH为8.0;高浓度(25mmol/L) EDTA使其拮抗活性降低62％;该抗菌物质对Cu2+、Fe3+敏感,对蛋白酶K不敏感.枯草芽孢杆菌8-32产生的抗菌物质具有一定的耐热、耐碱(pH 11.0)、对蛋白酶不敏感等特性.

One strain 8-32 which had strong antagonistic ability against to pathogens of soybean root rot(Fusarium oxysporum) was screened from 237 strains preserved in our laboratory, and finally identified as Bacillus subtilis through physiological and biochemical experiments,16S rRNA gene sequence analysis.The biocontrol effect of 8-32 against to soybean root rot was studied under pot condition using F.oxysporum as indicator.The results showed that 8-32 can effectively control of soybean root rot diseases, the prevention effect of up to 32.08%, and the 8-32 can obviously promote the growth of soybean seedlings (7.7%~15.8%); the content of chlorophyll increased by 7.8%~12.9%; POD activity increased by 11.6%~63.8%; 8-32 had obvious biocontrol effect on soybean root rot caused by F.oxysporum with application potential in the further.