为进一步挖掘四川、山东产区烟叶致香物质含量特色,本研究基于51份烤烟种质,在四川、山东两产区开展了烟叶8类致香物质含量检测分析比较工作.结果显示,整体上看,四川多酚类、叶绿素降解物绝对和相对含量高于山东产区,苯丙氨酸降解物、高级脂肪酸、多元酸绝对和相对含量低于山东产区;从不同基因型上看,四川92.2％以上种质多酚类、叶绿素降解物的绝对和相对含量高于山东产区,76.5％以上种质苯丙氨酸降解物、高级脂肪酸和多元酸的绝对和相对含量低于山东产区,进一步印证了两产区致香物质含量在整体上表现出的特点;51份种质中,四川产区有76.5％烤烟种质预测具有清香型风格倾向,在山东产区有90.2％烤烟种质预测具有中间香型风格倾向,多酚类和叶绿素降解物含量较山东产区高可能是四川清香型风格烟叶的重要物质基础;苯丙氨酸降解物、高级脂肪酸和多元酸含量较四川产区高可能是山东中间香型风格烟叶的重要物质基础.基因型和生态条件对香型指数均有较大影响,生态条件影响更大.

In order to clarify the usability of SSR molecular markers closely linked to sugar esters in the actual breeding of tobacco, and to accelerate the genetic improvement of flue-cured tobacco sugar esters, in this study, using polyacrylamide gel electrophoresis and GC-MS detection techniques, 5 tobacco germplasms with different genetic backgrounds and a genetic population with 192 recombinant inbred lines were used as experimental materials for screening and testing of 5 SSR molecular markers. The results showed that, among the 5 molecular markers, the polymorphisms of 2 molecular markers PT20315 and PT30354 were stable in different genetic backgrounds, and the correspondence between their polymorphic bands and the phenotypic difference of sugar esters were better. In the recombinant inbred line population, the consistency rate between the genotypes identified by the 2 molecular markers and phenotypes of sugar esters reached 93.75% and 90.58% respectively. The 2 molecular markers could be used in the molecular-assisted breeding of high-sugar ester tobacco. Currently, using of the 2 molecular markers in assisted selection has directionally improved new lineage materials such as high sugar ester K326 etc.