To clone and verify the cinnamate 4-hydroxylase（C4H） gene in tea plant and predict the biological function of C4H gene. Extracting total RNA from fresh tea leaves, and the C4H gene of tea was cloned by RT-PCR. The conserved domain, physical and chemical properties, subcellular localization, transmembrane domain and signal peptide, homology and phylogeny of the protein encoded by C4H gene were analyzed by bioinformatics software. The results showed that the C4H gene of tea contained a complete open reading frame（ORF） of 1518 bp, encoding 505 amino acids; conserved domain analysis showed that the protein encoded by tea C4H gene contained a domain of P450 superfamily protein（PLN02394）, with a molecular weight of 58.16 kDa and a theoretical isoelectric point of 9.29. It was a hydrophilic and unstable protein, localized in the endoplasmic reticulum, without signal peptide and transmembrane domain. The secondary structure of the protein was mainly a-helix and random coil. The C4H gene had the highest homology with oil tea at the amino acid level（92%）. In this study, the C4H gene of tea was successfully cloned and its biological function was analyzed, which laid a preliminary theoretical foundation for further study on the function of C4H gene and the molecular mechanism of tea synthesis.

本文基于matlab语言和线性规划模型,建立了一种新的杂交水稻种子纯度预测的数学模型,并利用matlab GUI工具设计了杂交水稻种子纯度预测软件界面程序.在已知a/A拷贝数比的情况下,可以直接由软件计算出待测水稻种子纯度范围,适用于杂交水稻种子纯度快速初筛.