目的:探讨lncRNA LSINCT5调控miR-451对肺癌细胞侵袭、迁移及顺铂(DDP)敏感性的影响.方法:以肺癌A549细胞为研究对象,使用LSINCT5特异性siRNA、DDP(4μg/ml)、si-LSINCT+DDP+miR-451 inhibitor处理细胞,不经特殊处理的细胞为空白组,qRT-PCR检测LSINCT5和miR-451 mRNA表达;Transwell小室检测细胞侵袭和迁移能力;Western blot检测E-cadherin、Vimentin和N-cadherin表达;双荧光素酶报告基因检测试剂盒测定LSINCT5和miR-451的靶向关系.结果:在转染LSINCT5 siRNA的A549细胞中,LSINCT基因表达明显降低(P<0.05).LSINCT5 siRNA及DDP均可明显抑制A549细胞侵袭、迁移能力,上调E-cadherin表达,下调Vimentin和N-cadherin表达(P<0.05),LSINCT5 siRNA及DDP联合使用对细胞侵袭、迁移及E-cadherin、Vimentin和N-cadherin表达影响明显强于单独使用LSINCT5 siRNA或DDP(P<0.05).LSINCT5和miR-451存在靶向关系,LSINCT5可负向调控miR-451的表达.抑制LSINCT5可通过上调miR-451增强DDP对A549细胞侵袭、迁移的影响.结论:lncRNA LSINCT5可通过上调miR-451抑制肺癌细胞侵袭、迁移及DDP敏感性.

目的 比较呼吸道病毒抗原、抗体检测及二者联合检测在呼吸道感染老年患者中的临床应用价值.方法 收集2019年1-12月该院收治的呼吸道感染老年患者的血清和鼻咽拭子,利用直接和间接免疫荧光法分别检测呼吸道病毒的抗原和抗体IgM,对比分析抗原、抗体及其联合检测的阳性率,并分析患者性别、年龄、病程对其检测结果的影响.结果 725例患者中抗原检测、抗体检测、联合检测的总阳性率分别为36.0％、27.0％、42.9％,3种检测方法的总阳性率比较差异有统计学意义(P<0.05).不同病程患者抗原检测、抗体检测阳性率比较差异有统计学意义(P<0.05).发病≤2 d、发病3～4 d、发病5～6 d患者抗原检测阳性率均高于抗体检测阳性率(P<0.05),但其联合检测阳性率与抗原检测阳性率比较差异均无统计学意义(P>0.05);发病≥7 d患者联合检测阳性率明显高于抗原、抗体检测阳性率(P<0.05).结论 呼吸道感染老年患者发病早期呼吸道病毒抗原检测优于抗体检测,与抗原检测相比,抗原、抗体联合检测没有明显提高阳性率;抗原、抗体联合检测可以显著提高发病≥7d患者呼吸道病毒检出阳性率.

Objective:To investigate the osteogenic effect of exosomes derived by mouse brain microvascular endothelial cell line bEnd.3 on bone marrow mesenchymal stem cells (BMSCs).Methods:Ultracentrifuge was used to extract the exosomes derived by brain-derived endothelial cells.3 (bEnd.3) cells bEnd.3 cells derived exosomes (b-Exo). Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting were use to characterize the exosomes. Alizarin red staining was used to detect the osteogenic effects of the exosomes. The relative messenger ribonucleic acid (mRNA) expression of osteogenic-related genes runt-related transcription factor 2 (Runx2), osteopontin and osteocalcin was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).Results:TEM showed that the particles presented a typical vesicle-like shape. NTA manifested the concentration of Exo was 7.5×10 10 particles/ml and the diameter was (100.0±7.3) nm. The b-Exo could enhance the BMSCs matrix mineralization and increase the mRNA expression of osteogenic-related genes Runx2, osteopontin and osteocalcin compared to the controls (8.28±0.34 vs. 3.71±0.40, t=4.974, P<0.01; 9.19±1.91 vs. 4.09±0.88, t=5.562, P<0.01; 10.31±1.67 vs. 5.32±0.31, t=5.432, P<0.01). Conclusion:The b-Exo could promote the osteogenic performance of BMSCs and may be applied to the bone regeneration and the cell-free treatment of osteoporosis in the near future.

目的 观察经皮空心钉微创治疗APC-Ⅰ型骨盆前环损伤的临床疗效.方法 通过观察2017年6月至2019年8月采用经皮空心钉微创治疗APC-Ⅰ型骨盆骨折前环损伤17例患者的手术时间、手术出血量、术后下地活动时间、骨折愈合时间、骨盆骨折复位Matta影像学标准评价复位质量及Majeed评分系统来评价功能状态和临床疗效.结果 所有患者均获随访,随访时间为8～13个月,平均10.7个月.单侧耻骨支或耻骨联合固定手术时间为28～47 min,平均35 min;出血量4～14mL,平均(7.1±2.4) mL;术后下地活动时间4～11周,平均(6.4±0.7)周,骨折愈合时间4～14周,平均(8.2±2.1)周.Matta复位标准结果,优13例,良4例.所有患者骨折均愈合,无切口感染、无神经血管损伤.末次随访时Majeed功能评分为(85.1±2.4)分,其中优13例,良4例.结论 经皮空心钉微创治疗APC-Ⅰ型骨盆骨折前环损伤能获得良好的临床效果.

Objective To investigate the biological significance of the change of the two VLDLR isforms in the developing fetal tissues. Methods We detected the distribution of the two isoforms of VLDLR in different fetal tissues and amplified the whole cDNA of VLDLR Ⅱ from fetal human brain by reverse transcriptase-PCR. The products were linked with pcDNA3. 0 vector to construct pcDNA-VLDLR Ⅱ recombinant. Expression plasmid containing VLDLR Ⅱ were introduced into ldl-A7 cells, and stable transformants were obtained by placing the cells in selective medium which contained G418. Finally, the ligand-binding affinity of ldl-A7 cells stably expressing VLDLR was determined with DiI-labeled VLDL. Results It was found that there was an significant difference in ligand-binding affinity between VLDLR Ⅱ and VLDLR Ⅰ . The ligand-binding affinity of VLDLR Ⅱ was approximately half that of VLDLR Ⅰ . Conclusion Biologically, the functional difference of the two forms of VLDLR may play a unique role in the developing cerebrum.