为了探讨三种细菌混合灭活疫苗对黄颡鱼的免疫保护作用,在防病上提供理论依据,将分离自患病黄颡鱼的温和气单胞菌、恶臭假单胞菌和类志贺邻单胞菌3种细菌浓度均调至约5×108 CFU/mL,按1:1:1的比例混合均匀成混合细菌悬液,然后制成福尔马林混合灭活疫苗,对黄颡鱼(体重约14.1g±1.2g)进行免疫保护试验,分别在免疫注射后7d、14d和28d进行3种细菌混合活菌的人工感染试验,以检测混合灭活疫苗的保护率.试验结果,使用0.5％福尔马林在25℃条件下灭活菌液24h可达到完全灭活要求,在人工感染后的第1d～3d为各试验组和对照组黄颡鱼的死亡高峰期,在免疫注射的7d、14d和28d后,混合灭活疫苗的免疫保护率依次为64.2％、92.4％和89.0％.表明本试验的3种细菌混合灭活疫苗对黄颡鱼具有一定的保护作用,在免疫注射14d后保护率达到最高.

This experiment was conducted to investigate the effects of dietary grape procyanidins on growth performance and immune function in broiler chickens.Two hundred and forty day-old Arbor Acre male broiler chickens were randomly allotted into four dietary treatments with different inclusion(0,7.5,15 or 22.5 mg/kg) of grape procyanidins.The experiment period lasted for 6 weeks.The results showed that chicks fed higher dose of grape procyanidins intake more feed and grew faster during the first 3 weeks.During the second 3 weeks and the whole 6 weeks,chicks fed 7.5 mg/kg grape procyanidins had higher body weight gain and superior feed efficiency,and chicks fed 15 mg/kg grape procyanidins diet had inferior growth performance compared to the controls.Dietary grape procyanidins did not affect the growth of the immune organs.The percentages of CD3+,CD4+,CD8+,αβ and γδ T lymphocytes in peripheral blood had the increased tendency in chicks fed 7.5 mg/kg grape procyanidins.Overall,optimal dose of grape procyanidins in the diet would stimulate late growth and modulate immune function in broiler chickens.

MicroRNA（miRNA）是一类内源性单链非编码小分子RNA，其长度为19～24个核苷酸.能与特定的信使RNA靶向结合，在转录后水平调控基因表达.miRNA广泛参与信号转导通路，发挥多种生物学作用.本文就动物miRNA与信号转导通路的研究进展作一综述.。