Objective: This study aimed to investigate the effects of 6 kinds of probiotics and their different adding ways on the production of rice wine. Methods: The probiotic rice wine was prepared by adding Lactobacillus rhamnosus BV-77, Lactobacillus fermentans TF-331, Lactobacillus saliva AP-32, Lactobacillus johnsoni MH-68, Lactobacillus acidophilus TYCA-06 and Lactobacillus casei CS-773. To compare the effects of saccharifying enzyme alone, saccharifying enzyme and yeast at the same time and adding methods（probiotic bran koji and probiotic powder） on alcohol content, reducing sugar, total acid and sensory quality of rice wine. Results: Yeast can promote the fermentation of probiotic rice wine and significantly improve the alcohol content and reducing sugar content of rice wine. The rice wine was fermented at 30 ℃ for 3 days by adding 200 U/g saccharifying enzyme and 0.04% yeast, with the highest alcohol content and reducing sugar content of 7.20%vol and 270.00 mg/mL, respectively, and the lowest total acid content of 8.40 mL/L. The rice wine is relatively rich in taste. Conclusion: Lactobacillus fermentans is the most suitable probiotics for rice wine brewing among 6 kinds of probiotics.

为探索酿酒微生物的相互作用,以高温大曲中分离得到的两株蜡样芽孢杆菌(Bacillus cereus Frankland)为研究对象,对其进行生化分析,探究其与酿酒酵母(Saccharomyces cerevisiae)混合发酵的效果.研究结果表明,蜡样芽孢杆菌的最适生长温度为37℃、最适pH值为7.0,其中B-1菌株耐乙醇能力达到16％vol;蜡样芽孢杆菌与酿酒酵母在固态和液态环境中均可混菌发酵,混合发酵时,先接入酿酒酵母后接入蜡样芽孢杆菌有利于两种菌的相互作用,混合固态发酵更有利于酿酒酵母和蜡样芽孢杆菌混菌发酵,能促进苯乙醇、乙酸、愈创木酚、四甲基吡嗪等多种风味物质产生,有利于提升白酒风味.

为了提高浓香型白酒中己酸乙酯和丁酸乙酯的产量,本文以从浓香型窖泥中已筛出的拜氏梭菌(Clostridium beijerinckii)、广西梭菌(Clostridium guangxiense)、煎盘梭菌(Clostridium sartagoforme)3株产酯性能较好的菌株为试验材料,对3株菌进行混合培养试验,并通过Box-Behnken响应面试验设计方法对各菌株的复配条件进行优化,并确定最佳复配条件.结果表明:3株菌两两之间均有一定的协同作用,同时添加3株菌复配发酵效果最优,己酸乙酯和丁酸乙酯含量显著高于其他协同发酵试验结果(p<0.05);复配发酵中3株菌接种量最佳配比为Y1:Y2:Y3=4:1.5:4.5,在此优化培养条件下,己酸乙酯产量可到达32.25 mg/100 mL,比优化前(19.78 mg/100 mL)提高了0.63倍;丁酸乙酯产量可到达117.68 mg/100 mL比优化前(45.37 mg/100 mL)提高了1.59倍,优化后己酸乙酯和丁酸乙酯含量显著高于优化前(p<0.05).可以通过发挥微生物间的协同作用,达到提高白酒风味物质的目的.

Chinese liquor industry for the exploration of Monascus focus in high yield strains, high temperature resistant strains specific strains screening and Monascus secondary metabolites of research, from the level of molecular biology to explore function of Monascus gene is still in the primary stage. According to the current liquor industry development situation of Monascus and enterprise development of the diversiifed needs, make corresponding exploration in Monascus gene function, health care wine Monascus, Monascus crude enzyme preparations, in order to improve the product quality, product variety, reduce the cost, bring economic beneifts for the enterprise, to meet consumer requirements.