Objective To investigate the expression and significance of HDAC 1 and E2F1 in renal clear cell carcinoma (RCCC).Methods Immunohistochemistry was used to detect the expression levels of HDAC1 protein and E2F1 protein in 51 cases of RCCC tissues and 24 cases of peritumoral normal tissues.RT-PCR was used to detect the relative expressions of HDAC1 and E2F1 mRNA in31 cases of RCCC tissues and 14 cases of peritumoral normal tissues.Moreover the correlation between the expressions of HDAC1, E2F1 and clinicopathological characteristics of RCCC was analyzed.Results Immunohistochemical staining results showed that positive expression rate of HDAC1 protein in RCCC tissues was significantly higher than that in peritumoral normal tissues (62.7%vs 25%, P <0.05).The positive expression rate of E2F1 protein in RCCC tissues was significantly higher than that in peritumoral normal tissues (70.6% vs 37.5%, P <0.05).The expressions of HDAC1 and E2F1 protein were not correlated to patient’s sex,age and tumor sizes ( P >0.05),however, which were related with histological grade and clinical stage ( P <0.05).RT-PCR results demonstrated that the relative expression levels of HDAC1 mRNA in RCCC tissues were significantly higher than those in peritumoral normal tissues (03.51 ± 0.151vs 0.102 ±0.044, P <0.05),furthermore, the expression levels of E2 F1 mRNA in RCCC tissues were significantly higher those in peritumoral normal tissues (0.318 ±0.092 vs 0.135 ±0.032, P <0.05).The relative expression levels of HDAC1and E2F1 mRNA were not correlated to patient’ s sex,age and tumor sizes ( P >0.05),however,which were related with histological grade and clinical stage ( P <0.05).Conclusion The expression levels of HDAC1 and E21F in RCCC are obviously higher than those in peritumoral normal tissues, which are correlated to histological grade and clinical stage,moreover, which are increased with the increase of malignant degree of tumor,which may be closely related with the progress of RCCC.

Objective To investigate the expression and clinical significance of E2F1 and EGR-1 in renal clear cell carcinoma (RCCC).Methods Immunohistochemistry was used to detect the expression levels of E2F1 protein and EGR-1 protein in 48 cases of RCCC tissue and 28 cases of cancer adjacent tissues.The correlation between the expressions of E2F1, EGR-1and clinicopathological characteristics of RCCC was analyzed.RT-PCR was used to detect the expression levels of E2F1 and EGR-1mRNA in 34 cases of RCCC tissues and 15 cases of cancer adjacent tissues.Results Immunohistochemistry results showed that the positive expression rate of E2F1 in RCCC tissues was significantly higher than that in cancer adjacent tissues (75%vs 32.1%, P <0.05).The positive expression rate of EGR-1 in RCCC tissues was significantly higher than that in cancer adjacent tissues (79.2%vs 35.7%, P <0.05).The expression of E2F1 was positively correlated to that of EGR-1 protein in RCCC tissues ( r =0.533, P <0.01),which was not related with patient’s sex and age ( P >0.05),however, which was closely correlated to histological grade and clinical stage of RCCC ( P <0.05) .RT-PCR results showed that there was a significant difference in the integral absorbance ratio of E2F1 between RCCC tissues and cancer adjacent tissues (0.2628 ±0.0656 vs 0.1225 ±0.0237, P <0.05),moreover, there was a significant difference in the integral absorbance ratio of EGR-1 between RCCC tissues and cancer adjacent tissues (0.2788 ±0.0893 vs 0.1279 ±0.02660, P <0.05). Conclusion The high expressions of E2F1 and EGR-1 in RCCC may be closely correlated to the pathogenesis, development, infiltration and metastasis of RCCC.