为了实现基于计算机视觉技术建立农产品无损检测系统,本文阐释了一套基于OpenCV和MPC平台的玉米果穗轮廓提取系统.首先通过玉米果穗侧面图像,对图像进行预处理及边缘提取,得到相应的玉米果穗表面几何特征参数值;其次获取玉米果穗穗粒分布图,通过对研究对象进行阈值分级,将每一区域相似的果穗粒分割,并提取出穗粒,最后转化确定后的像素值,获取玉米果穗穗粒分布图.根据玉米果穗二值图像自动检测得到的外部几何特征和玉米穗粒分布区域图像,为玉米果穗的识别提供了新的方法,加快了果穗识别速度,为玉米烤种、育种提供新的参考.最后针对以上研究成果,构建玉米果穗轮廓提取网站.
The aim of this study was to develop a simple and accurate isocratic RP-HPLC analytical method to qualify and quantify three flavonol glycosides(Quercetin,Kaempferol and Isorhamnetin) and four terpene lactones(Ginkgolide A,Ginkgolide B,Ginkgolide C and Bilobalide) of ginkgo leaf extract.The contents of flavonoid glycosides and terpene lactones from EGBN were measured by dialysis method.And the saturation solubility of flavonoid glycosides was also measured with this HPLC method.The condition of HPLC method for flavonol glycosides is: column: Agilent HC-C18(4.6 mm×250.0 mm,5 μm);mobile phase: ACN-0.4% phosphoric acid(28∶72,v/v).The drug elution was performed at 40 ℃ at a flow rate of 1.0 mL/min and detected at the wavelength of 360 nm.And the condition of HPLC method for terpene lactones is: column: Agilent HC-C18(250.0 mm×4.6 mm,5 μm);mobile phase: Methonal-THF-Water(46∶7∶47,v/v/v).The flow rate was kept at 0.8 mL/min.The temperature of NEB and EVA was performed at 60 ℃ and 105 ℃,respectively.The HPLC methods for flavonol glycosides and terpene lactones were shown to be linear,precise,sensitive,selective and accurate.And the contents of flavonoid glycosides and terpene lactones from EGBN can be measured by dialysis method.
目的 研制氨茶碱缓释微丸,并对其进行了质量评价并考察氨茶碱缓释微丸的制备工艺,缓释包衣层处方的组成.方法 以流化床包衣造粒的方法制备了氨茶碱缓释微丸.采用单因素摸索的方法确定了空白丸心的制备工艺并且对丸心的粉体动力学性质作了评价;同样单因素方法确定了氨茶碱缓释微丸的包衣处方和制备工艺,制得12h持续释放的缓释制剂,通过体外释放试验并对释放曲线进行曲线方程拟合,研究了微丸的缓释机理.结果 运用液相层积法制备空白丸心,通过制备工艺的摸索,制得的丸心粉体动力学性质良好,质量符合生产要求.同样通过液相层积法载药,制得含药量为36%的载药丸心.选用乙基纤维素为包衣材料对氨茶碱缓释微丸进行包衣,在较优制备工艺和包衣处方下制得微丸,测得体外释放曲线并对释放结果进行曲线拟合,发现其释药行为接近一级动力学方程.结论 流化床造粒包衣法制备的氨茶碱缓释微丸体外释放行为符合一级动力学方程;与市售氨茶碱片相比,自制缓释微丸的Tmax显著延长,Cmax明显降低,达到了良好的缓释效果.