The analysis of the nucleotide sequences of the cluster ITS1; 5.8S rRNA; ITS2 for Prunus persica (peach) and Prunus armeniaca (apricot), selected by the analysis of international genomic databases. With the use of bioinformatics methods have been designed oligonucleotide primers and tested for specificity. Experimental studies have been conducted on work designed primers for real-world objects. We used the self-obtained DNA preparations of peach and apricot fresh fruits of different varieties, as well as in the samples after the preliminary heat treatment of the samples. The optimization of the parameters of the polymerase chain reaction. The possibility of using designed oligonucleotide primers for species identification of peach and apricot in the heat-treated products of polymerase chain reaction. The obtained sequences of primers designed allow you to work more on the creation of methods of identification of fruit and berries for food.
The behavior of cinnamic acid in the presence of ion-pair reagents has been studied. Conditions have been chosen for the selective determination of cinnamic acid by capillary zone electrophoresis with normal and reversed polarity and spectrophotometric detection; the results have been applied for the analysis of drinks and milk hydrolysates. The influence of surfactants (sodium dodecyl sulfate, sodium octyl sulfonate) present in the buffer electrolyte on the efficiency of cinnamic acid determination is discussed.
The authors have carried an investigation of various samples of cattle carcasses and other products of agricultural raw materials for the presence of normal prion protein to identify their potential infectivity
In work the biochemical structure of preparations pantoram-C and pantogematogen from processing products of reindeer antlers to assess the possibility of their subsequent use for enrichment of dairy products with the preventive purpose is investigated
The possibility of application of denaturing polyacrylamide gel electrophoresis for definition the quality of the pasteurised whole milk was investigated
DNA isolation from many kinds of plants is considered a difficult problem because of high concentration of secondary metabolites, such as polysaccharides and polyphenolums. Vegetative DNA isolation from the food stuffs, received on their basis, is a problem even more difficult. It is connected with the presence of proteins, fats and inhibitory agents in assays. There are some procedures for the decision of this prob-lem, also commercial panels are developed. In the research we have lead analysis of existing procedures of DNA isolation from plants and isolation the vegetative DNA from food stuffs on their basis.