Objective To analyze the application effect of personalized psychological nursing in the perioperative period of patients with lung cancer. Methods The research subjects were 88 patients with lung cancer who underwent surgical treatment in our hospital from October 2020 to October 2021. They were divided into an experimental group and a control group by random number method, with 44 cases in each group. The experimental group was given individualized psychological care, and the control group was given routine care. The two groups were compared in terms of self-rating anxiety scale (SAS) score, self-rating depression scale (SDS) score, postoperative recovery, incidence of complications and nursing satisfaction. The t test was used for normal measurement data, and the x2 test was used for count data. Results Before nursing, there was no significant difference in SAS and SDS scores between the two groups (P＞0.05); after nursing, the SAS and SDS scores in the experimental group were significantly lower than those in the control group (P＜0.05). The drainage time, exhaust time, out-of-bed activity time and hospital stay in the experimental group were significantly shorter than those in the control group (P＜0.05). The incidence of complications in the experimental group was significantly lower than that in the control group (P＜0.05). The nursing satisfaction of the experimental group was significantly higher than that of the control group (P＜0.05). Conclusion Perioperative personalized psychological nursing for lung cancer patients has outstanding effect, can effectively improve the negative emotions of patients, play a role in promoting postoperative recovery, help to establish a good nurse-patient relationship, and reduce the incidence of complications.

通过生物信息学分析发现了米曲霉（Aspergillus oryzae）中属于氨肽酶M18家族的新成员天冬酰胺氨肽酶（AAP）基因,经反转录克隆获得该基因,实现了其在毕赤酵母GS115中的表达,同时研究了重组AAP的酶学性质及其在大豆蛋白水解中的应用。结果表明,重组AAP的分子质量约54.0 kDa,在较广的pH范围内有活性（pH 6.0～9.5）,最适反应温度和pH分别为50℃和7.0,Zn 2+ 和Ca 2+ 对该酶起促进作用,EDTA能够显著抑制该酶的酶活。以Asp-pNA为底物时,K m 为0.42 mmol/L,V max 为21.57μmol/（mL·min）,该酶具有水解N末端为天冬氨酸和谷氨酸的底物特异性。重组AAP可显著降低大豆多肽的苦味,提高水解度,具有在食品中的应用潜力。