On the basis of one cDNA-AFLP expressed sequence tag(EST) from longan(Dimocarpus longan Lour.) cotyledon embryos which is 84%homologous to Antirrhinum majus,the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was cloned by rapid-amplification of cDNA ends(RACE).The gene consisted of 1395 bp encoding a protein of 336 amino acids,with 71 bp and 316 bp at 5′-UTR and 3′-UTR,respectively.The amino acid sequence comparison with the GAPDH gene of Oryza sativa,Vitis vinifera,Musa acuminata,Arabidopsis thaliana,and Ginkgo biloba showed that identity was all higher than 85%.The sequence was accepted and released by GenBank(accession number: FJ694011).