Objective:We analyzed the clinical data of patients treated by colonoscope assisted transcolonic specimen extraction surgery, summarized the clinical treatment experience, and explored its clinical feasibility.Methods:The clinical data, surgical methods, pathology, and follow-up results of 8 colonic neoplasm patients treated with transcolonic NOSES in the First Affiliated Hospital of Zhengzhou University from January 2016 to May 2022 were analyzed retrospectively.Results:The tumors were completely resected in all 8 patients, and the specimens were successfully extracted through anus by colonoscope. The median operation time was 190 (160, 210) minutes. The median intraoperative blood loss was 60 (40, 80) mL. The median VAS pain score was 4 (2, 5) on the first day after operation. The median number of lymph nodes retrieved was 13 (12, 14). There was one case of pulmonary infection on the fourth day after operation, and one case of ileus on the sixth day after operation. Both recovered after conservative symptomatic treatment. No patient had anastomotic leakage and abdominal infection. Follow up for 6 to 50 months showed no implantation, recurrence, or death.Conclusion:Colonoscope assisted transcolonic specimen extraction surgery is a safe, reliable and effective surgical method with excellent application prospects and clinical value.