以环境友好型絮凝剂代替碱性铅粉进行糖料甘蔗试验分析,为减少糖料甘蔗品质分析中碱性铅粉的使用提供参考.结果表明,13种絮凝剂中,以乙酸锌和氢氧化钙的过滤效果相对较好;复合型絮凝剂(乙酸锌+氢氧化钙)中乙酸锌和氢氧化钙加入量与蔗汁比例分别为0.5%和0.4%时,可代替碱性铅粉满足糖料甘蔗试验要求,加入混合絮凝剂后有利于沉降速度的提高,减少蔗汁在沉降池中的停留时间,甘蔗品质参数蔗汁糖度、蔗糖分检测结果与碱性铅粉处理结果误差为:蔗汁糖度0.001～0.008(允许误差0.01),蔗汁蔗糖分0.001～0.006(允许误差0.02),满足国家标准GB/T 10499-2014《糖料甘蔗试验》要求.

Objective: The paper aims to establish a dual-wavelength spectrophotometry method for the determination of the content of amylose and amylopectin in yam and taros, and to provide basic data for variety selection, quality grading, product processing and standard formulation. Method: The spectrum of amylose and amylopectin were studied and the maximum absorption wavelength and reference wavelength were determined. The color conditions such as dispersant and acetic acid and measuring conditions were optimized. The absorbance values of the color complexes were determined. Result: The standard curve equation of amylose was y=0.0259x−0.0051，R2=0.9994, with the linear range of 0~33 μg/mL. The standard curve equation of amylopectin was y=0.0031x+0.0167，R2=0.9965, with the linear range of 0~111 μg/mL. The limits of detection and quantification of amylose were 0.093 μg/mL and 0.28 μg/mL respectively. The limits of detection and quantification of amylopectin were 0.079 μg/mL and 0.24 μg/mL, respectively. The recovery of amylose was 90.0%~100.0%. The recoveries of amylopectin were 88.0%~108.0%. Conclusion: The dual-wavelength spectrophotometry method was simple and feasible, accurate and reliable. It was suitable for the determination of amylose and amylopectin in yam and taros.