OBJECTIVE:To observe the effect of acupuncture at "Feishu" (BL 13) + "Dingchuan" (EX-B 1) and "Kongzui" (LU 6) + "Yuji" (LU 10) for the airway remodeling in asthma rats based on the transforming growth factor-β1 (TGF-β1)/ Smad family member 3 (Smad3) signaling pathway; and explore the efficacy difference between the two acupoint combinations.METHODS:Forty SPF male SD rats, aged 4 weeks, were randomly divided into a blank group (n = 10) and a modeling group (n = 30). The ovalbumin (OVA) sensitization method was used to establish asthma model in the modeling group. After successful model preparation, the rats of the modeling group were randomized into a model group, an acupuncture at "Feishu" (BL 13) + "Dingchuan" (EX-B 1) (AAF) group, and acupuncture at "Kongzui" (LU 6)+"Yuji" (LU 10) (AAK) group, with 10 rats in each one. Starting from day 15 of the experiment, 5 min after motivating, acupuncture was applied to "Feishu" (BL 13) + "Dingchuan" (EX-B 1) and "Kongzui" (LU 6)+"Yuji" (LU 10) in the AAF group and the AAK group respectively. The intervention was delivered for 30 min each time, once daily, lasting 3 weeks consecutively. Using lung function detector, the airway resistance (RL) and dynamic compliance (Cdyn) of the lungs were detected. The histomorphology of lung tissues was detected with HE staining and Masson staining, and the mRNA and protein expression of TGF-β1 and Smad3 in lung tissues was detected with the real-time PCR and Western blot methods.RESULTS:Compared with the blank group, RL was increased and Cdyn was decreased in the rats of the model group (P<0.01); and RL was reduced and Cdyn was increased in the AAF group and the AAK group when compared with those in the model group (P<0.01, P<0.05). The rats of the model group had bronchial lumen stenosis, inflammatory cell infiltration, collagen fibre hyperplasia and thickened smooth muscle in the lung tissues when compared with those in the blank group; and in comparison with the model group, all of the above morphological changes were attenuated in the AAF group and the AAK group. Besides, these morphological changes of the lung tissues were more alleviated in the AAF group when compared with those in the AAK group. In comparison with the blank group, the mRNA and protein expression of TGF-β1 and Smad3 of the lung tissues was increased in the model group (P<0.01), and it was reduced in the AAF group and the AAK group when compared with that in the model group (P<0.05, P<0.01). The mRNA expression of TGF-β1 and Smad3 was lower in the AAF group when compared with that in the AAK group (P<0.05).CONCLUSION:Acupuncture at either "Feishu" (BL 13)+"Dingchuan" (EX-B 1) or "Kongzui" (LU 6)+"Yuji" (LU 10) reduces the airway remodeling in the rats with asthma, which may be related to the down-regulation of mRNA and protein expression of TGF-β1 and Smad3. The better efficacy is obtained with acupuncture at "Feishu" (BL 13)+"Dingchuan" (EX-B 1).

目的 本研究围绕"虚拟现实康复"进行系统的文献计量和可视化分析,探索虚拟现实康复的发展状况、研究热点,为虚拟现实康复提供研究及参考.方法 利用CiteSpace软件,本研究对从中国知网(CNKI)检索到的493篇虚拟现实康复相关文献进行可视化分析.结果 ①相关文献跨度为1988-2020年,虚拟现实康复的关注度在不断地上升.②主要关键词和关键词聚类分析得到19个聚类,关键词图谱由401个节点,649条连线组成,主要聚类结果包括虚拟现实技术、康复训练、脑机接口、虚拟现实、常规康复治疗等.③《虚拟现实技术在运动康复中的应用》是热点论文被引最多的.④从作者分布图来看,发文量排名前三的张成杰、杜豪、杨岩在虚拟现实康复研究领域做了较多工作,同时,重庆理工大学在该领域的发文量靠前,作者和研究机构的合作度不高.⑤《中国康复医学杂志》《中国康复理论与实践》《中华物理医学与康复杂志》杂志的载文数量较多.结论 通过对CiteSpace虚拟现实康复的可视化分析,发现虚拟现实康复呈现出阶段性发展,逐渐成为学术热点,要加强学术合作,推进虚拟现实技术介入临床应用.