Austenitic stainless steel (SUS316L) surfaces were processed with a Nano-second Laser System (NLS). Changing processing parameters such as defocus distance, scanning rate and path intervals caused different effects on topography of the surfaces. The processed surface showed unique surface microstructure with complicated micro holes. The wettability of the surfaces was also examined by measuring a static and dynamic contact angle. The contact angles were changed from 0° to 137°, and the contact angle histereses decreased to 3.6° depending on the processing parameters. This suggests that the wettability of SUS316L steel surfaces can be controlled by NLS processing.