<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的观察维生素E（VE）对四氯化碳（CC14）作用下大鼠肝星状细胞（HSC）增殖和细胞外基质（ECM）产生的影响。方法采用原位灌注方法分离大鼠HSC。结果CC14作用下大鼠HSC3H-脯氨酸（3H-Pro）的掺入显著增加,Ⅲ型前胶原（PCⅢ）、透明质酸（HA）、板层素（LN）及纤维连接素（FN）的分泌水平明显提高。200nmol/LVE预处理4h可有效抑制CC14作用下大鼠HSC3H-胸腺密啶（3H-TdR）、3H-Pro的掺入,VE保护能使CC14作用下HSC分泌PCⅢ、HA、LN和FN减少与HSC产生丙二醛（MDA）下降,二者呈正相关。结论VE可抑制CC14作用下大鼠HSC增殖和通过抗氧化损伤抑制HSCECM的分泌。</span>
Objective Study the inhibitory effects of selenium on the production of basic lipid peroxidation and the secretion of extracellular matrix(ECM) of hepatocytes and ito cells in vitro.Methods Rat hepatocytes and ito cells were isolated and cultured.Results The results showed that selenium could obviously lower the production of malondialdehyde(MDA) and raise the activity of glutathione peroxidase(GSH Px) and inhibit the secretion of precollagen type Ⅲ (PCⅢ) in hepatocytes and ito cells as well as inhibit the secretion of hyaluronic acid in ito cells,and that there was a negative correlation between the level of PCⅢ and the ratio of GSH Px/MDA in both cells and a negative correlation between the levels of hyaluronic acid and the ratio of GSH Px/MDA in ito cells.Conclusions These studies indicated that selenium could lower the production of lipid peroxidation and inhibit the secretion of ECMs by raising the activity of GSH Px of rat hepatocytes and ito cells. [
