目的 探讨"双主教学模式"的多元整合教学法在中西结合妇产科学中的应用教学效果.方法 将海南医学院中医学院中西医结合专业2015级本科班设为对照组,采用传统教学模式;2016级本科班设为研究组,采用"双主教学模式"的多元整合教学法,最终通过期末成绩、综合成绩和学生满意度调查进行教学评价.结果 研究组的期末成绩和综合成绩均高于对照组(P<0.05),调查问卷显示绝大部分学生对该教学法的满意度较高,研究组在提高课程学习效果、加强师生交流、提高分析归纳与总结问题能力、提高团队合作能力、增强对理论知识的掌握、培养临床思维能力、提高中西医妇产科知识的结合能力7个方面的调查满意度均高于对照组(P<0.05).结论 "双主教学模式"的多元整合教学法通过多种教学法之间整合优劣、互相渗透和叠加优势,显著优化了教学过程,提高了教学质量,从而增强了学生解决问题的能力.

Accumulating evidence has demonstrated that transcriptional regulation is affected by DNA methylation. Understanding the perturbation of DNA methylation-mediated regulation between transcriptional factors (TFs) and targets is crucial for human diseases. However, the global landscape of DNA methylation-mediated transcriptional dysregulation (DMTD) across cancers has not been portrayed. Here, we systematically identified DMTD by integrative analysis of transcriptome, methylome and regulatome across 22 human cancer types. Our results revealed that transcriptional regulation was affected by DNA methylation, involving hundreds of methylation-sensitive TFs (MethTFs). In addition, pan-cancer MethTFs, the regulatory activity of which is generally affected by DNA methylation across cancers, exhibit dominant functional characteristics and regulate several cancer hallmarks. Moreover, pan-cancer MethTFs were found to be affected by DNA methylation in a complex pattern. Finally, we investigated the cooperation among MethTFs and identified a network module that consisted of 43 MethTFs with prognostic potential. In summary, we systematically dissected the transcriptional dysregulation mediated by DNA methylation across cancer types, and our results provide a valuable resource for both epigenetic and transcriptional regulation communities.

Autophagy is a self-degradation process that maintains homeostasis against stress in cells. Autophagy dysfunction plays a central role in the development of tumors, such as colorectal cancer (CRC). In this study, autophagy-related differentially expressed genes, their downstream functions, and upstream regulatory factors including RNA-binding proteins (RBP) involved in programmed cell death in the CRC were investigated. Transcription factors (TFs) and miRNAs have been shown to mainly regulate autophagy genes. Interestingly, we found that some of the RBP in the CRC, such as DDX17, SETDB1, and POLR3A, play an important regulatory role in maintaining autophagy at a basal level during growth by acting as TFs that regulate autophagy. Promoter methylations showed negative regulations on differentially expressed autophagy gene (DEAG), while copy number variations revealed a positive role in them. A proportional hazards regression analysis indicated that using autophagy-related prognostic signature can divide patients into high-risk and low-risk groups. Autophagy associated FDA-approved drugs were studied by a prognostic network. This would contribute to the identifications of new potential molecular therapeutic targets for CRC.