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大气细颗粒物(fine particulate matter,PM2.5)是重要的环境健康危险因素.PM2.5暴露对包括肺部感染,异常肺功能和肺癌在内的呼吸系统疾病产生重要影响.目前已经提出了参与PM2.5不良作用的几种机制,包括氧化应激、DNA损伤、炎症反应和上皮-间充质转化(epithelial-mesenchymal transition,EMT)等[1-2].其中,EMT在多种细胞命运决定和转移过程中发挥重要作用,引起广泛关注.有机物是PM2.5的重要化学组分,其在健康相关研究最多的是多环芳烃(Polycyclic aromatic hydrocarbon,PAHs)及其衍生物.

Objective It is crucial to study the possible health effects of household air pollution (HAP).Methods Eligible rural (n = 58) and county women (n = 10) were enrolled in this study as research and control groups to explore the relationship between the HAP and potential health effects on rural women in northwest China. Firstly, a questionnaire on general characteristics of subjects was conducted. Next, in the non-heating (September 2019) and heating seasons (March 2020), the average concentrations of respirable particulate matter 10 (PM10) and fine particulate matter (PM2.5) in living rooms of rural subjects were monitored and health indicators of all subjects were measured. The levels of Malondialdehyde (MDA), 1-Hydroxypyrene (1-OHP),and producing 8-hydroxy-2-deoxyguanosine (8-OHdG) and the activity of Superoxide dismutase (SOD) of all subjects in urine samples in two seasons were detected.Results The questionnaire results revealed that economic level, house structure, local lifestyle, and seasonal fuel energy type were influence factors relatively high exposure to HAP during the heating season, and all subjects had a certain of self-management of blood pressure. However, there were deficiencies in rural subjects in terms of regular checkups and medication taking. The average PM10 and PM2.5 concentrations during the heating season were significantly higher than those in the non-heating season. Meanwhile, systolic blood pressure (SBP) and Diastolic blood pressure (DBP) of the rural subjects showed significant seasonal changes and SBP was significantly higher than those in the county subjects during the heating season. The 1-OHP, MDA, SOD, and 8-OHdG levels of rural subjects increased significantly in the heating season compared with the non-heating season and county subjects (P < 0.01).Conclusion Rural women in northwest China were exposed to higher HAP due to lifestyle in heating season, which could cause changes in blood pressure as well as oxidative stress levels. Therefore, it is important to further explore the mechanism on oxidative stress involved in high blood pressure induced by HAP.

Inflammation is widely recognized as an essential inducer of epithelial-mesenchymal transition (EMT). Meanwhile, competitive endogenous RNA (ceRNA) has been involved in a variety of disease processes. Therefore, the aim of the current study is to explore the regulation of ceRNA in the PI3K/AKT pathway and EMT mechanism in inflammatory response caused by low molecular weight-polycyclic aromatic hydrocarbons (LMW-PAHs). The A549 cells were treated with an equal mixture of phenanthrene (Phe) and fluorene (Flu), and total RNA was extracted for transcriptome sequencing. The target regulation of ceRNA hsa_circ_0039929/hsa-miR-15b-3p_R-1/FGF2 was further determined for mechanism study. The mixture of Phe and Flu significantly upregulated the expressions of hsa_circ_0039929 and FGF2, down-regulated hsa-miR-15b-3p_R-1, activated the PI3K/AKT pathway and promoted EMT. Mechanically, the overexpression of hsa-miR-15b-3p_R-1 inhibited the expressions of hsa_circ_0039929 and FGF2, reversed the activation of PI3K/AKT signaling pathway by LMW-PAHs, and blocked the occurrence of EMT progression. Furthermore, knockdown of hsa_circ_0039929 could promote the levels of hsa-miR-15b-3p_R-1, while inhibit the expression of FGF2. The effects of hsa_circ_0039929 knockdowns on PI3K/AKT pathways and EMT progress resembled the hsa-miR-15b-3p_R-1 overexpression. All above suggested that ceRNA hsa_circ_0039929/hsa-miR-15b-3p_R-1/FGF2 played an important role in the inflammation and EMT caused by LMW-PAHs.