Background/Aims: Androgen receptor (AR), estrogen receptor α (ERα) signaling and their interaction with epidermal growth factor receptor (EGFR) signaling pathway is a potential therapeutic target in non-small cell lung cancer (NSCLC). To explore the cross communication between AR, ERα and EGFR signaling pathway, we used RNA silencing technology and sex hormones intervention in NSCLC cell line. Methods: Model system was the well-characterized A549 adenocarcinoma NSCLC cells which can express AR and ERs well. We used different concentrations of testosterone (T), estradiol (E2) intervention cells and small interfering RNA (siRNA) specifically targeting AR, ERα in A549 cells, then examined the expression of AR, ERα and EGFR/PI3K/AKT axis, followed by detection of cells proliferation, apoptosis, migration and invasion. Results: After knocked down the expression of AR, ERα, the EGFR/PI3K/AKT axis was inhibited, and the proliferation decreased, apoptosis increased, migration and invasion decreased in A549 cells. 50µM T and 50µM E2 intervention inhibited AR, ERα and EGFR/PI3K/AKT axis expression, and the proliferation decreased, apoptosis increased, migration and invasion decreased in A549 cells. 10µM T, 10µM E2 and 100µM T, 100µM E2 intervention promoted the expression of AR, ERα and EGFR/PI3K/AKT axis, which enhanced the activity and migration invasiveness of A549 cells. Conclusions: These data provide a rationale for further investigation of the antitumor activity of clinical sex hormones therapy and the development of anticancer drugs targeting sex hormone receptors in the clinic. Future .

目的:探究微小RNA-140-3p(miR-140-3p)对程序性细胞死亡配体1(PD-L1)的靶向关系以及对非小细胞肺癌A549细胞的活力、迁移和侵袭的影响.方法:使用RT-qPCR检测HLF-1、A549和H1299不同细胞株中miR-140-3p的表达水平,选择差异最显著的A549细胞用作后续研究对象;TargetScan软件预测和双萤光素酶报告基因实验验证miR-140-3p和PD-L1之间的靶向关系;RT-qPCR和Western blot检测转染miR-140-3p模拟物和抑制剂对PD-L1表达水平的影响;MTT法检测miR-140-3p和PD-L1对A549细胞活力的影响;Transwell实验检测miR-140-3p和PD-L1对A549细胞迁移和侵袭的影响.结果:miR-140-3p在人肺癌A549和H1299细胞的表达中显著下调(P<0.05);miR-140-3p高表达能够使PD-L1表达下调,对A549细胞的活力、迁移和侵袭具有抑制作用;转染pcDNA3.0-PD-L1能够阻断miR-140-3p对A549细胞活力、迁移和侵袭的抑制作用.结论:miR-140-3p可通过靶向负调控PD-L1抑制A549细胞的活力、迁移和侵袭.

目的 探讨小干扰RNA(siRNA)沉默雌激素受体β(ER)表达和雌二醇(E2)干预对非小细胞肺癌细胞系A549的作用及其可能分子机制.方法 siRNA转染实验分为空白对照组(Control组)、siRNA-NC转染组(NC组)、Lipofectamine 2000转染组(Lipo组)和siRNA-ERβ转染组(siRNA组);E2干预实验分为0μmol/L组、10 μmol/L组、50 μmol/L组和100 μmol/L组.采用Western blot和RT-PCR分别检测A549细胞中ERβ、表皮生长因子受体(EGFR)、磷脂酰肌醇3-激酶(PI3K)、Akt的蛋白和mRNA表达,CCK-8法和划痕实验分别检测A549细胞活性和迁移能力的变化.结果 与Control组、NC组和Lipo组相比,siRNA组ERβ、EGFR、PI3K、Akt蛋白和mRNA表达下调,细胞活性和迁移能力降低(P＜0.05).与0 μmol/L组相比,10 μmol/L组ERβ、EGFR、PI3K、Akt蛋白和mRNA表达上调,细胞活性和迁移能力增加(P＜0.05);而50μmol/L组ERβ、EGFR、PI3K、Akt蛋白和mRNA表达下调,细胞活性和迁移能力降低(P＜0.05);100 μmol/L组各指标均无明显变化(P＞0.05).结论 E2及其受体ERβ可能通过EGFR/PI3K/Akt信号通路影响A549细胞的活性及迁移能力,从而参与非小细胞肺癌的发生和发展过程.

Objective:To observe the characteristics of TCM Syndromes of vasovagal syncope,summarize the characteristics and regularities of vasovagal syncope,and to investigate the TCM syndrome regularity and pathogenesis of vasovagal syncope.Methods:By carrying out the epidemiological investigation and field questionnaire survey,100 cases of vasovagal syncope were collected and the relationship between syndrome factors was analyzed by factor analysis and cluster analysis.Results:① The main TCM clinical symptoms of vascular vagal syncope are:syncope,being dizzy,pale complexion,cold limbs,palpitations,chest tightness,nausea,tinnitus,bad eye sight,fatigue,irritability,being easy to catch cold,sweating,insomnia,soreness and weakness of waist and knees,limbs burnout,bitter mouth and dry throat,abundant expectoration,fat,feeling of chill,poor appetite,limbs numbness,flushed face,throat discomfort,gomphiasis,headache,vomiting,depressed,abdominal distention and short of breath.② The main TCM syndromes of vasovagal syncope are:Wind Jue belongs to Liver Wind Syndrome;Heat Jue belongs to Yin Deficiency and Excessive Fire Syndrome;Deficiency Jue belongs to Qi Deficiency Syndrome.Conclusion:Vasovagal syncope includes Wind Jue,Phlegm Jue,Heat Jue and Deficiency Jue,which is related to Liver,Kidney,Heart and Spleen as well as the the pathologic factors such as Phlegm,Dampness,Heat and Deficiency.

目的 掌握河南口岸出入境人员心血管疾病的发病情况及特点,以利于加强国际旅行卫生保健健康咨询工作.方法 对河南口岸2014年1月至2015年12月58633名出入境人员的心电图检查资料进行统计和分析.结果 检出心电图异常3902人次,检出率6.655％.结论 出入境检验检疫机关在加强对传染病监测的同时,应加强对直接威胁人体健康和旅行安全的非传染性疾病特别是心血管疾病的监测,以更好地为出入境人员服务.

BACKGROUND/AIMS:In contrast to men, women have experienced a rapid increase in lung cancer mortality. Numerous studies have found that the sex differences in lung cancer are due to reproductive hormones. Experiments in female mice with and without ovariectomy were performed to explore the possible mechanism by which sex hormones (and their receptors) influence lung cancer.METHODS:Twenty-four female C57BL/6 mice aged 56-62 days were randomly divided into the ovariectomized group and the control group. In the ovariectomized group, the bilateral ovaries were removed via the dorsal approach, while the control group underwent a sham operation with bilateral ovarian fat resection at the same sites. After 3 weeks of recovery, Lewis lung cancer cells were transplanted into these mice by subcutaneous inoculation of a tumour cell suspension to establish the ovariectomized lung cancer model. Beginning on the 6th day after subcutaneous inoculation, mouse weight and transplanted tumour volume were measured every 3 days. After 3 weeks, all the mice were killed by cervical dislocation, and we measured the tumour weight. Mouse serum and tumour tissues were removed. Then, the serum levels of E2 (oestradiol) and T (testosterone) were detected by ELISA; the protein expression levels of AR (androgen receptor), ERα (oestrogen receptor α) and ERβ (oestrogen receptor β) were detected by Western Blot and IHC (immunohistochemistry); and the mRNA expression levels of AR, ERα and ERβ were detected by qRT-PCR (quantitative real-time polymerase chain reaction) in the ovariectomized and control groups.RESULTS:Compared with the control group, both mouse weight and transplanted tumour volume increased rapidly in the ovariectomized group, and the transplanted tumour weight was significantly heavier in the ovariectomized group (1.83±0.40 and 3.13±0.43, P<0.05). E2 and T serum levels decreased exponentially in the ovariectomized group, while the E2/T ratio increased compared with the control group (E2: 55.88±11.45 and 78.21±9.37; T: 0.82±0.14 and 1.46±0.16; ratio: 69.62±14.43±29.81 and 52.22±5.42; all P<0.05). The Western blot and IHC results indicated that AR, ERα and ERβ protein expression levels were obviously higher in transplanted tumour and lung tissues from the ovariectomized group, with particular increases in ERβ in transplanted tumour tissue and in ERα in lung tissue. The PCR results also showed markedly higher mRNA expression levels of AR, ERα and ERβ in the ovariectomized group, and in particular, ERβ in transplanted tumour tissue and ERα in lung tissue were significantly increased in the ovariectomized group.CONCLUSION:Ovariectomy decreased E2 and T serum levels and increased the E2/T ratio in mice, and this imbalance in the internal environment promoted the growth of transplanted tumours. Sex hormone disorder not only promoted transplanted tumour growth but also significantly reduced the protein and mRNA expression levels of sex hormone receptors. The metabolism of E2 and T may affect the growth, proliferation and metabolism of lung cancer cells, and the mechanism by which sex hormones and their receptors influence lung cancer is worthy of further research.

Objective:To investigate the difference of tumor growth,the expression levels of sex hormones and their receptors in male castrated mice and normal mice transplanted with Lewis lung cancer,and to explore the possible mechanism of sex hormones in the occurrence of lung cancer.Methods:The male C57BL/6 mice aged 8-9 weeks were randomly divided into the castrated group (n =12) and control group (n =12).The castrated group underwent bilateral orchiectomy via the abdominal approach,while the control group underwent a sham operation of bilateral testicular fat removal at the same sites.After 2 weeks of restorative feeding,these mice were transplanted with Lewis lung cancer tissues by subcutaneous inoculation to establish the kidney deficiency and lung cancer xenograft model.The tumor growth in each mouse was observed,and the tumor size was measured every 5 days.After 3 weeks,all mice were weighed,and their serum was taken.Then the mice were sacrificed to remove the tumor and lung tissues,and the weights of tumor and lung tissues were measured,respectively.The serum levels of estrodiol (E2) and testosterone (T) were detected by ELISA method.The expression levels of androgen receptor (AR) and estrogen receptor α/β (ERα/β) were detected by immunohistochemistry.Resulu:Compared with the control group,the body weight of mice in the castrated group lost more after operation (P ＜ 0.05),while the volume of tumor increased faster (P ＜ 0.05),the weights of tumor and lung tissues increased (both P ＜ 0.05),the number of lung metastatic nodules also increased significantly (P ＜ 0.05),and the serum levels of E2 and T decreased greatly (both P ＜ 0.001).In the lung tissues,the expression levels of AR,ERα and ERβ were all high in the two groups,and the high-expression rates of AR,ERα and ERβ in the castrated group were especially higher than those in the control group (all P ＜ 0.05).In the tumor tissues,the high-expression rate of ERβ in the castrated group was higher than that in the control group (P ＜ 0.05),but the expression levels of AR and ERα in the two groups were not high.Conclusion:The castration may cause the disorder of sex hormone in mice,and abnormal up-regulation of the expression of relative sex hormone receptors,which can make the mice lose weight,promote the growth of transplanted tumor,increase the number of lung metastasis nodules.These suggest that the imbalance of sex hormones and relative receptors may promote the formation and development of lung cancer.

Objective To leam human immunodeficiency virus (HIV) infection and epidemiological characteristics among entry-exit personnel at Henan port,and to provide a scientific basis for the strengthening of AIDS at Henan port.Methods The data of all of the entry-exit persons with HIV antibody positive was analyzed,and the software SPSS 10.0 was used to complete the statistical analysis.Results There were 128 cases of HIV antibody positive detection in 400 085 entry-exit personnel,the prevalence rate was 0.032％.The epidemiological investigation showed that HIV antibody positive rate in different age groups was statistically significant (P=0.000＜0.05).There were 122 cases of male and 6 cases female.The most infection was labors and crew,sexual and blood transmission were the main transmission.Conclusion It should be strengthen AIDS health education in young entry-exit personnel.

目的 分析河南口岸近7年出境劳务人员疾病监测结果,了解其身体健康状况和疾病流行趋势,以采取有效的预防和干预措施.方法 采取回顾性研究的方法,对2009-2015年河南口岸出境劳务人员的疾病监测结果进行疾病的分类和患病率统计.结果 共检出主要疾病19种,其中非传染性疾病14种包括高血压、色盲、色弱、脂肪肝、肝硬化、肝占位、胆囊息肉、胆囊结石、胆囊占位、肾结石、肾占位、心肌梗死、频发性室性早搏、肺占位,检出率较高的为脂肪肝、高血压、色弱和胆囊息肉,检出率分别为16.497％、5.093％、4.139％、2.299％;传染性疾病5种包括肺结核、艾滋病、梅毒、乙型肝炎及丙型肝炎,检出率分别为0.058％、0.035％、0.389％、1.868％、0.391％.结论 应加强对出境劳务人员的健康体检结果监测,普及各种非传染性疾病和传染性疾病在出境劳务人员中的宣传教育,为出境劳务人员提供更多健康咨询,切实做好传染病防控工作,保障出境劳务人员身体健康.

目的 了解河南口岸出境人员疑似预防接种异常反应(AEFI)情况,为进一步做好口岸出境人员预防接种工作提供参考.方法 采用描述性流行病学方法对2013-2015年河南口岸出境人员AEFI报告情况进行分析.结果 共接到AEFI报告52例,AEFI平均发生率22.74/10万剂次.其中一般反应占不良反应的96.15％,异常反应占3.85％.白破疫苗发生率最高,麻疹-流行性腮腺炎-风疹联合减毒活疫苗次之;以留学生为主,女性多于男性;夏秋两季多发;82.69％在24小时内发病,所有病例预后良好.结论 AEFI一般反应多见,多种疫苗均可以发生.