OBJECTIVE:To investigate the role of multiple serological methods in the identification of complex antibodies.METHODS:The blood group antigens were detected by saline and microcolumn agglutination methods. The saline method was used to screen and identify IgM-type antibodies in the patient's serum, while the polybrene, anti-globulin, microcolumn agglutination, enzymic and absorption-elution methods were used to screen and identify IgG-type antibodies.RESULTS:The patient was B/CCDee/Jk(a-b+)/Fy(a-b+) blood type. The serum reacted with panel cells, and the reaction presented anti-E pattern in the saline medium. It was fully positive in the microcolumn agglutination card, except 2 negative ones after using papain to treat the panel cells. Referring to the pattern table, it was concluded that there existed anti-c, anti-E, and anti-Jka antibodies, and one antibody corresponding to an antigen that was easily destroyed by papain. The red blood cells with specific phenotype were selected for absorption-elution to identify IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies.CONCLUSION:It is confirmed that IgM-type anti-E, and IgG-type anti-c, anti-E, anti-Jka and anti-Fya antibodies exist in the patient's serum by multiple serological methods.

TransfusionEarly View ONE-PAGE REPORT OF NEW ALLELES OR ANTIGENS Identification of a novel FUT1 allele with c.325_414dup in a Chinese individual with para-Bombay phenotype Jinlian Liu, Jinlian Liu orcid.org/0000-0002-3189-4866 Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorLilan Li, Lilan Li orcid.org/0000-0002-0852-3661 Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorGuoguang Wu, Corresponding Author Guoguang Wu [email protected] Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, China Correspondence Guoguang Wu, Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi, China. Email: [email protected]Search for more papers by this authorTingting Ma, Tingting Ma Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorXuejun Liu, Xuejun Liu Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorBaojia Huang, Baojia Huang Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorJierun Chen, Jierun Chen Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorYan Zhou, Yan Zhou Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this author Jinlian Liu, Jinlian Liu orcid.org/0000-0002-3189-4866 Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorLilan Li, Lilan Li orcid.org/0000-0002-0852-3661 Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorGuoguang Wu, Corresponding Author Guoguang Wu [email protected] Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, China Correspondence Guoguang Wu, Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi, China. Email: [email protected]Search for more papers by this authorTingting Ma, Tingting Ma Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorXuejun Liu, Xuejun Liu Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorBaojia Huang, Baojia Huang Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorJierun Chen, Jierun Chen Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this authorYan Zhou, Yan Zhou Nanning Blood Center, Nanning Institute of Transfusion Medicine, Nanning, Guangxi, ChinaSearch for more papers by this author First published: 15 June 2023 https://doi.org/10.1111/trf.17452Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article. REFERENCES 1Race RR, Ruth S. Blood groups in man. 6th ed. Oxford: Blackwell Scientific Publications; 1975. p. 22–7. 2Liu JL, Liu XJ, Ma TT, et al. Serological identification and FUT1 gene mutation analysis of 8 individuals with Para-Bombay phenotypes in Guangxi. Chin J Exp Hematol. 2021; 152(4): 1318–24. 3Kelly RJ, Emst LK, Larsen RD, et al. Molecular basis for H blood group deficiency in Bombay(oh)and Para-Bombay individuals. Proc Natl Acad Sci U S A. 1994; 91(13): 5843–7. 4 International Society of Blood Transfusion(ISBT). Names for H (ISBT 018) blood group alleles. https://www.isbtweb.org/resource/018h.html 2023-03-31/2023-05-05. Early ViewOnline Version of Record before inclusion in an issue ReferencesRelatedInformation

目的 探讨电子交叉配血技术在红细胞不规则抗体阳性患者交叉配血中的应用.方法 收集2020年1月至2021年4月南宁市各医院送检的抗体筛查阳性和(或)交叉配血不相合的137例患者的137份血液标本,应用盐水法进行ABO、Rh血型和其他血型系统红细胞抗原检测,应用盐水法、聚凝胺法、抗球蛋白法、微柱凝胶卡法、酶法、吸收放散法等检测和鉴定红细胞不规则抗体并分析其特异性,以实验室建立的红细胞血型抗原/抗原基因型已知献血者数据库为基础,对红细胞血型抗原及不规则抗体特异性明确的患者实施电子交叉配血,采用血型血清学交叉配血进行验证.结果 137份标本抗体特异性涉及Rh、MNS、Lewis、Kidd和Duffy等5个血型系统,Rh系统抗体占比(56.93％)最高,其次为MNS系统抗体(18.25％).137份标本采用电子交叉配血结果与血型血清学交叉配血验证结果一致,未发生血型不合的输血反应.结论 电子交叉配血技术有助于为红细胞不规则抗体阳性患者快速和准确地找到相配合的血液,有望为输血的安全有效提供保障.