Objective:To investigate the expression of G2 and S phase-expressed-1 (GTSE1) in prostate cancer(PCa) tissues and its regulation in PCa metastasis.Methods:GEPIA, UALCAN and FireBrowse databases were analyzed to determine the correlation between GTSE1 expression and clinical indexes and metastasis in patients with PCa. The co-expression genes and possible pathways of GTSE1 were further analyzed. Wound healing and transwell invasion assays were used to detect the effect of GTSE1 on the migration and invasion of PCa cells in vitro. Western blotting assay was used to analyze the effects of GTSE1 on epithelial-to-mesenchymal transition (EMT) markers.Results:The expression of GTSE1 was significantly increased in PCa tissues, especially in metastatic PCa, and was closely related to the disease progression and lymph node metastasis. Overexpression of GTSE1 enhanced the ability of migration and invasion of PCa cells, and promoted the formation of EMT, which showed the opposite effects with GTSE1 knockdown. GTSE1 may act on the microtubules of PCa cells and cooperate with TPX2, TOP2A and CENPF to drive EMT and metastasis of PCa. Conclusion:GTSE1 is highly expressed in PCa tissues and can promote the invasion and migration of PCa cells, which may be developed as a new molecular target for the treatment of metastatic PCa.

Objective:To investigate the effect of negative pressure treatment combined with pelvic floor muscle training on urinary continence and sexual function of patients with prostate cancer (PCa) after radical prostatectomy.Methods:From October 2016 to April 2019, in the Third Affiliated Hospital of Sun Yat-sen University 70 patients were diagnosed with prostate cancer and underwent laparoscopic radical prostatectomy. According to the random number table method, they were divided into intervention group and control group, 35 cases each. The control group of 35 patients received routine nursing (pelvic floor muscle functional exercise), and the intervention group of 35 patients underwent routine nursing, and then increased negative pressure exercise therapy from six weeks after surgery. The urinary continence were compared at 6 weeks, 10 weeks, and 18 weeks. At the same time, the International Erectile Function Scale (IIEF-5) score and subjective well-being survey were conducted to observe the treatment effect.Results:The median scores of urinary incontinence at 6 weeks, 10 weeks, and 18 weeks in the intervention group were 2, 1 and 0, respectively, which were significantly lower than those in the control group. The recovery time of urinary control function was shorter than that of the control group, and the difference was statistically significant (P<0.05); In addition, the intervention group had a faster increase in IIEF-5 score and subjective well-being score than the control group, suggesting that the intervention group had postoperative erectile function. The improvement of the quality of life was significantly better than the control group.Conclusion:Negative pressure therapy combined with pelvic floor muscle training can significantly improve the urinary continence and sexual function of patients after radical prostatectomy, shorten their recovery time.

Objective:To evaluate the effect of integrative nursing care for prevention of deep venous thrombosis(DVT) of lower extremities in patients underwent radical prostatectomy.Methods:A total of 35 patients undergoing radical prostatectomy from April 2020 to August 2020 were recruited as the intervention group, and received integrative nursing care, including screening and assessment, observation of precursor symptoms,vascular protection, early postoperative rehabilitation activitie, position management, nutritional support and body fluid management, body temperature management and patient education, and other nursing measures. A total of 35 patients from September 2019 to March 2020 undergoing radical prostatectomy were selected as the control group and received routine nursing. The occurrence of DVT in lower extremities was compared between the two groups.Results:DVT occurred in 1 patients (2.86%) of the intervention group and 5 patients (14.28%) in the control group. The difference of the incidence of thrombosis between the two groups was statistically significant (P<0.05).Conclusion:The integrative nursing care can effectively reduce the incidence of DVT in lower extremities in patients undergoing radical protectomy and promote patient recovery.

Objective To investigate the level of readiness for discharge of patients after prostate cancer surgery based on the concept of Enhanced Recovery After Surgery (ERAS), and to explore its influencing factors, so as to provide references for improving the readiness for discharge of patients after prostate cancer surgery. Methods The general information questionnaire, the discharge preparation scale, and the discharge guidance quality scale were used to investigate 119 patients discharged from the urological surgery department of a tertiary A-level hospital in Guangzhou after radical prostatectomy. Results The total score of discharge readiness of patients after radical prostatectomy was 147.74 ± 35.71 points, which was at a lower middle level and the total score of discharge guidance quality was 180.68 ± 38.91 points, which was at a medium level. Multiple linear regression analysis showed that education level, family monthly income, Gleason score, whether to perform lymphatic dissection, whether to discharge with a urinary catheter, and the quality of discharge guidance were the main factors influencing the readiness for discharge of patients after prostate cancer surgery. Conclusion In clinical nursing work, it is necessary to implement individualized health education according to the characteristics and needs of different patients to improve the level of preparation for discharge of patients after prostate cancer surgery.

Objective:To investigate the role of Smad4 as a target gene regulated by microRNA (miRNA, miR)-301a and the mediating effect of high glucose in promoting the proliferation of prostate cancer cells.Methods:Real-time PCR was used to examine the expression of miR-301 in prostate cancer cells. Bioinformatics prediction by using software of TargetScan and PicTar and luciferase reporter gene assay was utilized for the identification of the target genes of miR-301. The expression of Smad4 was detected by real-time PCR and Western bloting [25 μg, 10% sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE)] after the miR-301 mimic was transfected 24 h later. After miR-301 mimics were transfected or co-transfected with Smad4 over-expression plasmid for 24 h, the proliferation and cell cycle of prostate cancer cells (PC-3 and DUl45) were examined by cell counting kit-8 (CCK-8) and flow cytometry, respectively.Results:Bioinformatics prediction and luciferase reporter gene assay demonstrated that Smad4 was the target of miR-301a. After up-regulating miR-301a, the expression of Smad4 mRNA and protein was significantly reduced. The cell proliferation ability increased by 160% ( P<0.05) after up-regulating the expression of both miR-301a and Smad4 for 96 h. MiR-301a could inhibit the expression of Smad4, thereby promoting the cell transition from the G 1 phase to the S phase. In PC-3 cells, before overexpression of miR-301a, the percentages of different phases were G 0/G 1 62.60%, S 28.00%, G 2/M 9.40%; after overexpression, the percentages were G 0/G 1 49.97%, S 41.04%, G 2/M 8.99%, the differences in the phases of G0 and S were significant ( P<0.05). In DU-145cells, before overexpression of miR-301a, the percentages of different phases were G 0/G 1 65.16%, S 24.54%, G 2/M 10.30%; after overexpression, the percentages were G 0/G 1 53.34%, S 36.67%, G 2/M 10.00%, the differences in the phases of G 0 and S were significant ( P<0.05). In addition, knockdown of Smad4 shortened the G 1 phase and prolonged the S phase, which was consistent with the results of miR-301a overexpression. Overexpression of Smad4 could block the effect of miR-301a inducing transformation of G 1/S phase. Conclusion:MiR-301 can regulate the proliferation of prostate cancer cells by targeting Smad4 gene. Smad4 plays an important role in the regulation of hyperglycemia associated prostate cancer.