The sensitivity and specificity of L-hCAR cell to group B Coxsackie viruses were studied. 43 prototype enteroviruses were at the same time separately inoculated onto the Hep-2 and L-hCAR cells. On L-hCAR cell, only group B Coxsackie viruses (Cox.B) propagated. These Cox.B prototype viruses were titrated on both Hep-2 and L-hCAR cells and their titers were similar. In virus isolation, 94 stool suspensions, known as non-polio enteroviruses, were reinoculated onto both the Hep-2 and L-hCAR cells. Within 14-day observation, 8 samples showed cytopathic effects(CPE) on L-hCAR cell. All these 8 isolates were identified to be Cox.B. The results showed that L-hCAR cell are sensitive and specific to Cox.B and it can be used to selectively isolate Cox.B from stool samples.