The present invention relates to a method for efficiently synthesizing or amplifying a nucleic acid containing the target nucleic acid sequence. In the process according to the invention, comprises 3 'sequence at the end portion (A) sequence that hybridizes to (Ac' of the target nucleic acid sequence) at the 3 'end portion, than the sequence in the target nucleic acid sequence (A) 5 a primer comprising a 5 'side of the' complementary sequence (Bc) sequence that hybridizes to (B sequences present (B) on the side ') the sequence (Ac)', the sequence (Ac ' the number of bases) and X, the number of bases of the region flanked by the sequence in the target nucleic acid sequence (a) and the sequence (B) and Y, the sequence (Ac ') and the sequence (B' with) certain number of bases intervening sequence between when the Y '(Y' can be a 0), the range of {X- (Y-Y ')} / X is -1.00～1.00 using primers.
liang ying lin qi (良英 林崎)合作论文数7jianwu jiujing (臼井健悟)合作论文数2xiong hui chuan jing (雄輝 川井)合作论文数1jinsen ji (基 金森)合作论文数1jian zhi hua jian (健志 花見)合作论文数1nalai lixu (豊須納瀬)合作论文数1xian shi gao qiao (憲史 高橋)合作论文数1yan wu gao qiao (研吾 高橋)合作论文数1he mei tian zhong (和美 田中)合作论文数1zheye tianbian (田邊哲也)合作论文数1ya ji ying jing (亜季 櫻井)合作论文数1hao zhi zhong yue (浩志 塚越)合作论文数1ji ye long tai lang (姫野 龍太郎)合作论文数1yu zhi hu gu (裕之 戸谷)合作论文数1tong huzhong (戸塚 統)合作论文数1zhi jiu shi chuan (智久 石川)合作论文数1gong yu qing shui (公裕 清水)合作论文数1yang ping gong qian (洋平 宮前)合作论文数1gui tai lang ping jing (圭太郎 平井)合作论文数1bo chen xiao chuan (博臣 小川)合作论文数1zhuji quan (泉 竹吉)合作论文数1da jie ji cheng (大介 吉成)合作论文数1MITANI YASUMASA合作论文数1alexander lezhava合作论文数1
Yoshihide Hayashizaki合作论文数Omics Science Center, Riken1Hajime Kanamori合作论文数1Alexander Lezhava合作论文数1Takeshi Hanami合作论文数1Yasumasa Kimura合作论文数1Takahiro Soma合作论文数1
