We studied the qualitative and quantitative composition, accumulation, and location of steroid glycosides in four strains of cell culture of Dioscorea deltoidea Wall. Reardless of activity of endogenous beta-glucosidases, all strains are characterized by the presence of steroid glycosides only in the furostanol form. The qualitative composition of steroid glycosides is the same for all four strains and is represented by four compounds: deltoside, protodioscin (aglycone diosgeinin), and their 25S analogs (aglycone yamogenin). The strains differ significantly in quantitative content of saponins, strain DM-0.5 being a superproducer in regard to these compounds (up to 12% of cell dry mass). The content of steroid glycosides is analyzed in cells of strain DM-0.5 and protoplasts obtained from it. These compounds are shown to be located in protoplasts and the free space of cells. their quantitative distribution varying with culture age.