Objective : to develop a model of a biomedical cell product that is consistent with the «homologous drug» strategy  based on protocols for preparing the cell component and scaffold carrier for preclinical studies on a large laboratory  animal (pig). Materials and methods . Biomedical cell products and skin equivalents (SE), were formed using  plasma cryoprecipitate prepared from blood plasma of healthy donors and mesenchymal stem cells (MSCs) of  human adipose tissue. Cryoprecipitate from pig blood plasma and human adipose tissue-derived MSCs were used   to form model skin equivalents (mSE). Bright-field microscopy, phase-contrast microscopy (Leica DMI 3000B)  and fluorescence microscopy (Cytation 5 imager; BioTek, USA) were used to monitor the state of cells in the  culture and in the composition of the equivalents. Scaffolds for equivalents were tested for cytotoxicity (MTT test,  direct contact method). The cell distribution density was characterized by author’s method (Patent No. 2675376  of the Russian Federation). Results . An mSE was developed for preclinical studies on a large laboratory animal  (pig). In the mSE, components that change from halogen to xenogenic conditions during transplantation to the  animal were replaced. A comprehensive approach to preparing mSE was presented. It includes sampling of primary  pig biomaterial, extraction and characterization of adipose tissue-derived MSCs, preparation of a scaffold  carrier for the corresponding «homologous drug» strategy. Cytotoxicity of the mSE scaffold was evaluated. It  was shown that mSE provides mechanical support (similar to SE) to cells, as well as comparable development of  cellular events during cultivation. Conclusion . A model of a biomedical cell product was developed. This model  is consistent with the «homologous drug» strategy for preclinical studies on a large laboratory animal (pig). The  paper presented a comprehensive approach to developing a model equivalent based on protocols for preparation  and testing of the cellular component, the scaffold carrier and the ready-to-use model equivalent.

Одним из важнейших аспектов регенеративной медицины является подбор матрикса — биологического каркаса тканеинженерной конструкции. Для наилучшей воспроизводимости структуры и свойств поврежденной ткани, а также поддержания клеточной адгезии и пролиферации оптимальным является использование матриксов, полученных при децеллюляризации нативных органов с последующей рецеллюляризацией различными клеточными линиями. Методами растровой электронной микроскопии в режиме естественной среды и сканирующей импульсной ультразвуковой микроскопии определена микроструктура нативных и децеллюляризированных матриксов дермальных тканей.

The results of studies representing the interaction of cells with various products provided for biomedical use are stated. It has been shown that wide-field fluorescence microscopy makes it possible to evaluate the cytotoxicity of the samples, the viability, morphology and proliferative activity of cells during the process of interaction, as well as the adhesion, migration, and distribution of cells, both on the surface of materials and in the structure of scaffolds and biomedical cell product. Methods of wide-field fluorescence microscopy make it possible to carry out intravital studies of the interaction of cells with materials without any additional processing and samples destruction.

Investigation results are presented on the influence of microwave noise radiation on proliferative and functional cellular activities of dermal fibroblasts of healthy and scar-damaged skin. The obtained data confirm the hypothesis on the different impact of microwaves on healthy and pathologically changed cells, as well as the importance of adaptive reactions at the level of neuro-immune-endocrine interactions in the course of connective tissue reparative regeneration.

This research analyzes expression of Toll-like receptors (TLR2 and TLR4) on CD14+ peripheral blood cells and evaluates their functional activity in patients with severe thermal injuries in the acute period of burn disease. TLR2 expression increase and TLR4 expression decrease (p

A new procedure for obtaining nanosized hydroxyapatite (Ca 5(PO 4) 3OH) powder is presented. The obtained compound has been tested on a human fibroblast cell culture to clarify its biological compatibility and activity. The synthesized hydroxyapatite is shown to be non-toxic to human dermal fibroblast cultures in vitro.

The mice of different lines were immunized twice with low doses (10(6) of sheep red blood cells (SRBC) at a 7-day interval. The immune spleen cells (ISC) from syngeneic and allogeneic mice preliminarily (14 days before) given SRBC (5 . 10(8) were transplanted into the animals at varying times before or after primary antigen administration. It was discovered that the administration of the ISC from CBA and DBA/2 mice to syngeneic mice 1 or 2 days after the priming drastically lowers the production of antibody-forming cells in the recipients in response to the repeated antigen injection. The ISC from C57BL/6 mice provided low suppressor effect. ISC transplantation one, three or six days after the priming exerted no effect on the height of the secondary immune response. Suppression of antibody formation was also obtained in the allogeneic combinations CBA ISC leads to leads to C57BL/6 and DBA/2 ISC leads to CBA. It is concluded that the spleen cells from mice immunized with SRBC are capable to suppress the development of the immunological memory of SRBC.