The assessment of possibility of medullar hemopoiesis restoration by means of transplantation of mesenchymal stem cells of marrow cultivated on the three-dimensional biodegraded matrixes from polylactic acid was performed on model of chronic myelosuppression under the influence of cytostatic preparation Cyclophosphanum at 30 rats. By means of hematological analysis it is established that hypodermic introduction of the tested scaffold with mesenchymal stem cells against myelosuppression leads to faster restoration of all sprouts of hemopoiesis, and also prevents emergence of secondary hypoplasia of marrow.

The article presents the results of a study of the effect of desynchronization of circadian rhythms on lipid metabolism in case of obesity. The model of the alimentary obesity was reproduced in 80 outbred male rats with an initial weight of330-380 g by changing the nature of nutrition. The animals were kept in cages for one individual within one month and were at high-calorie diet consisting of standard food (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%) (fats-29.6%, proteins -14.8%, carbohydrates 55.6%) (diet C11024, Research Dietes, New Brunswick, NJ). To induce desynchronosis, experimental animals were in artificial light or in full darkness for 10 days around the clock. All procedures with animals were performed in accordance with international rules and standards (European Communities Council Directives of 24 November 1986, 86/609/EEC). The study showed that keeping animals at high-calorie diet during 30 days promoted formation of the model of alimentary obesity, as evidenced by a significant increase in their body weight (average 43%). According to biochemical and enzyme-linked immunosorbent analyses of blood serum of rats, diet-induced obesity on the background of the round the clock lighting is accompanied by hypercholesterolemia and hypertriglyceridemia, tendency to hyperglycemia and decreased production of hormones insulin and leptin. Keeping experimental animals with the model of alimentary obesity under long-term continuous darkening, despite their increase in weight, promoted normalization of all the considered parameters. It is assumed that this effect may be conditioned by synthesis of the protective factor during hours of darkness. Thus, to improve the efficiency of obesity treatment, one can recommend shifting the natural light-dark cycle to darkness for non-pharmacological correction of metabolic disorders.

Purpose: to study the effect of the phase structure artificial calcium – phosphatic surfaces on an osteogenic differentiation of stromal stem cells in vivo on implants with the fixed range of roughness. Materials and methods. The condition of the surface was estimated by means of the measuring system Talysurf 5-120, phase structure – by the diffractometer Shimadzu XRD-7000 (Japan), element structure – by the electron microscope of Hitachi S5500. An implant from the titan of the VT1.0 brand (diameter of 12 mm, thickness of 1 mm) with the rough calcium phosphate coating applied by microarc oxidation with a column of bone marrow has been hypodermically placed to 35 mice males of the CBA/CaLac line. Histological research was carried out in 45 days. The quantitative parameters of grown tissue plates were defined by the computer morphometry. Results. The growth of coarse-fibered bone tissue with the cavities filled by red marrow has been revealed on plates with artificial surfaces. Its reproducibility was 22 % higher on surfaces composed of tricalcium phosphate and hydroxylapatite compared with amorphous calcium phosphate.