Purpose. To compare the experimental results of corneal repair and regeneration after transplantation of combined bioconstruction with cultivated or non-cultivated (minimally manipulated) buccal epithelial cells in total limbal stem sell deficiency. Material and methods. The study was conducted on 24 eyes of Gray Chinchilla rabbits. At the first stage rabbits underwent bilateral total limbectomy and mechanical de-epithelialization of the cornea, resulted in fibrovascular pannus development. After superficial keratectomy to transparent layers the combined bioconstructions with autologous cultivated epithelial buccal cells were placed on the corneas of group 1 (12 eyes), bioconstruction with non-cultivated (minimally manipulated) buccal epithelial cells were placed on corneas of group 2 (12 eyes). Temporary tarsorrhaphy was performed for 5 days. In the postoperative period the area of the de-epithelized surface, neovascularization and corneal transparency were evaluated. Results.In both groups we noted reduction of corneal erosion at all periods. The dynamics of recovery processes was equal, the difference between the groups was statistically significant only at 15th and 60th days (p = 0.028 and p = 0.026). Groups did not differ in neovascularization severity and corneal transparency (p > 0.05). Conclusion.Due to the absence of obvious differences in cornea restoration, the use of non-cultivated epithelial cells of the oral mucosa seems to be a promising treatment method in treatment of total limbal stem sell deficiency. For wide application in ophthalmological practice, further study of their effectiveness in clinical trials is required. Keywords: limb, limbal stem cell deficiency, epithelium of the oral mucosa, cornea, rabbits

Purpose: to determine the concentration of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in the tear of patients with cornea melting and to assess whether these factors can be used as prognostically significant biomarkers of keratolysis. Material and methods. 20 patients who underwent urgent penetrating keratoplasty were tested for cornea perforation (melting) before and after the procedure. For control, 12 healthy adults (24 eyes) were tested. The tear fluid was collected before the surgery and on the 7th day after it with the help of filtering paper. MMP-9 and TIMP-1 concentrations were determined by ELISA. The course of the early postoperative period was assessed according to the area of keratotransplant epithelization on the 7th day after surgery. Results. MMP-9 and TIMP-1 concentrations in the tears of all patients before the surgery were significantly higher than the ones of healthy controls. The patients who were supposed to have re-keratoplasty had a significantly higher MMP-9 concentration than the ones who expected the first keratoplasty. An uncomplicated early postoperative period showed a statistically significant decrease of MMP-9 and TIMP-1 concentrations, whilst the complicated one demonstrated a significant increase of both markers. The highest increase of both markers was shown by the patients who underwent re-keratoplasty. Conclusion. MMP-9 concentration in the tears is a valid marker of the clinical course of the early post-keratoplasty period. To ensure a successful donor cornea engraftment, the levels of MMP-9 and TIMP-1 in the tear must be high enough before the surgery and the concentration of the ferment should decrease timely after its main functions have been completed. A 2-fold (or greater) decrease of MMP-9 concentration in the tear on the 7th day after keratoplasty can be considered a marker of the favorable prognosis for engraftment.

Purpose. To estimate the effectiveness of using of platelet-rich plasma lysate (PRP lysate) for prevention and treatment keratolysis in patients after urgent keratoplasty. Material and methods. To compare the effectiveness of treatment with PRP lysate after urgent keratoplasty we formed the comparison group and the study group (20 people in total). Each group included 10 patients: 5 with corneal perforation and 5 with graft lysis. After urgent keratoplasty all patients received standard treatment. 10 patients from the study group received platelet-rich plasma lysate in subconjunctival injections and instillations. The assessment of the condition of the cornea was carried out using clinical and instrumental studies, including visometry, biomicroscopy with fluorescein staining, photoregistration and OCT on the Heidelberg Engineering “OCT Spectralis” apparatus. Results.Treatment with PRP lysate in all patients of the study group led to 100 % epithelization of the graft up to 7 days after surgery. The average area of graft epithelization on day 7 was 55,9 ± 12,9 % in patients before the first keratoplasty, and 38,22 ± 14,5 % in patients after rekeratoplasty (p < 0,01 compared with the study group. In all patients of the study group, the graft was preserved. In the comparison group, 70 % of patients had keratolysis in the long term after surgery. Conclusions. The use of PRP lysate after urgent keratoplasty may be a promising method for preventing and treating keratolysis. Keywords: keratoplasty, matrix metalloproteinases, tissue inhibitor of matrix metalloproteinases, platelet-rich plasma

Purpose. The aim of the work is to compare the time of epithelization and the change in the thickness of the cornea in the treatment of erosions after corneal burns of 2–3 degrees and ulcers after keratitis with preserved amnion and amnion saturated with autologous PRP lysate. Materials and methods. To compare the effectiveness of preserved amnion and lysate-soaked PRP, 2 groups of patients were identified: the main group (amnion saturated with lysate PRP) and the comparison group (amnion without lysate PRP). Each group included patients with erosions after recent burns with an average area of 60 % of the cornea and ulcers after keratitis with a corneal thickness at the bottom of the ulcer of 382 microns in the experimental group and 393 microns in the comparison group. In the comparison group, ulcers were treated by covering the cornea with an amniotic membrane for 7–14 days, after which the amnion was removed and the condition of the cornea was monitored. An amniotic membrane saturated with PRP lysate was used in the experimental group. PRP lysate was made from autologous blood of patients in the laboratory of cell transplantation and immunotyping of the N.V. Sklifosovsky Research Institute of Emergency Medicine using a patented technology. To do this, platelet-rich plasma (PRP) with a platelet content of over 1000 thousand/ml was isolated from the blood of donors, which was then frozen at -80 °C and thawed at 0–4 °C in order to destroy cell membranes. Assessment of the condition of the cornea was carried out using clinical and instrumental studies, including biomicroscopy with fluorescein staining, photoregistration and OCT on the Heidelberg Engineering “OCT Spectralis” apparatus. Results. The use of an amniotic membrane saturated with PRP lysate led to epithelialization of erosions after recent burns in 1.3 months and a decrease in corneal edema by 247 microns, epithelialization of ulcers after keratitis in 1.3 months and an increase in the thickness of the cornea at the bottom of the ulcer by 62.5 microns. Treatment with conventional preserved amnion took 1.8 months before complete epithelization in recent burns with a decrease in corneal stroma edema by 193 microns, and epithelization of ulcers after keratitis required 1.6 months and ended with an increase in the thickness of the cornea at the site of the ulcer by 42 microns. Conclusion. The study showed that the presented method of treating erosions and ulcers of the cornea of various genesis using an amniotic membrane saturated with autologous PRP lysate allows achieving complete and persistent epithelialization in a shorter time and with fewer amnion coatings, unlike the comparison group. At the same time, patients do not need additional conservative treatment of epitheliopathies.

Purpose: to evaluate the content of tissue inhibitor of matrix metalloproteinase type 1 (TIMP-1) in platelet preparations obtained in different methods.Material and methods. Platelet-rich plasma (PRP), platelet-poor plasma, and a suspension of platelets washed from plasma (WP) were isolated from the blood of 10 volunteer donors, and a morphofunctional analysis of platelets was performed. After that, a platelet lysate was prepared from PRP, WP, and platelet-poor plasma. The concentration of TIMP-1 in the lysates was determined using enzyme immunoassay.Results. The level of TIMP-1 in the lysates of PRP and WP did not differ significantly; in platelet-poor plasma, the level of TIMP-1 was significantly lower than in the lysates of PRP and WP (p = 0.003). In all types of preparations, the concentration of TIMP-1 was 2-4 times higher than similar values in the blood serum of healthy people, as reported in other studies. A weak correlation was found between the TIMP-1 concentration and the platelet number in PRP, and there was no correlation between the content of platelets with granules and the TIMP-1 level in the lysates. The presence of leukocytes in the initial PRP also did not affect the TIMP level in the final lysates. A strong correlation was found between the TIMP-1 concentration in the WP lysate and in platelet-poor plasma, as well as a direct correlation between the total platelet content and the TIMP-1 level in the PRP lysate.Conclusions. Preparations with high concentrations of TIMP-1 can be obtained both from concentrated platelet suspensions and from platelet-poor plasma. To optimize the method, it is necessary to study the effect of platelet preparations with TIMP on various biological models.

Introduction . The review is devoted to the actual problem of treating patients with keratolysis. The role of matrix metalloproteinases in the pathogenesis of cornea and corneal graft melting is discussed as well as the practical experience of using synthetic metalloproteinase inhibitors in various branches of medicine and in ophthalmology, in particular. In the field of eye diseases, the search for effective methods for the treatment of corneal injuries of various origins, as well as its post-transplant complications, has been underway for a long time. Recent studies have shown that local imbalance of matrix metalloproteinases and their inhibitors system, as well as the immune system status, may play the main role in the outcome of urgent keratoplasty, and the use of synthetic metalloproteinase inhibitors can significantly improve the biological result of the donor cornea transplant. The role of platelets in the regulation of the proteolytic system has not been fully studied. However, some literature data on the platelet-associated inhibitor of metalloproteinases and the use of platelet-rich plasma to correct the collagenolytic activity of enzymes are of great interest to ophthalmologists, due to therapeutic efficacy and simple method of producing its production the autologous platelet-rich plasma.The present brief literature review covers the pathogenesis and clinical features of keratolysis, factors which can affect the outcome of urgent keratoplasty, describes the features of matrix metalloproteinases, their inhibitors, and the plateletrich plasma as a potential endogenous source of a tissue inhibitor of matrix metalloproteinases. Aim . To evaluate the possibility of using inhibitors of matrix metalloproteinases for keratoplasty based on a literature review. Material and methods . To write the review article, we have made the search in the homeland eLibrary.RU database and in the PubMed resource database to select the articles on the topic published in the period from 1985 to 2022.

To date impossibility of keratoplasty with category IV and V corneal opacifation, keratoprosthetics is the only available option to restore useful vision. The most commonly implanted keratoprostheses nowadays are keratoprosthesis «Boston 1 and 2», osteoodontokeratoprosthesis, Fedorov-Zuev keratoprosthesis («MICOF»). This article highlights a comparative evaluation, outcomes and complications of different types keratoprosthesis listed before. Results of implantation of keratoprosthesis «Boston 1 and 2», Osteoodontokeratoprosthesis, Fedorov-Zuev keratoprosthesis «MICOF» (China) collected through literature analysis. The results of implantation of the Fedorov-Zuev keratoprosthesis at the Helmholtz National Medical Research Center for Eye Diseases gained with our own clinical data analysis. In efficacy comparison of keratoprosthesis, we got the following conclusions: when implanting the Boston type keratoprosthesis, functional results are better, fewer complication rates (such as secondary glaucoma and vitreous hemorrhage), but the anatomical outcome is not that good (high necrosis and rejection rates). With osteoodontokeratoprosthesis there are good functional outcomes, but overgrowth of the optical cylinder and vitreous hemorrhage can be seen often. When implanting the FedorovZuev keratoprosthesis using the Helmholtz National Medical Research Center for Eye Disease methods we declare good functional result comparable to osteoodontokeratoprosthesis and the best anatomical result (absence of cases of tissue necrosis over the keratoprosthesis, rejection, exposure of the keratoprosthesis). Keywords: keratoprosthesis, keratoprosthesis, implantation

Purpose : to compare the effectiveness of conventional preserved amnion and amnion saturated with platelet-rich (PRP) plasma lysate for the treatment of persistent corneal erosions (PCE) after high-risk keratoplasty. Materials and methods . 40 patients with persistent corneal erosions after high-risk penetrating keratoplasty, followed up for 12 months, were divided into two clinical groups of 20 people each. The main group of patients, aged 34 to 84, received Flexamer amniotic membrane + PRP lysate, while the comparison group, aged 41 to 80, received Flexamer amniotic membrane only. Amniotic membrane coating was used in persistent corneal erosions of penetrating keratoplasty after unsuccessful conservative treatment. The amnion was sewn 2 mm from the limb with a continuous suture and covered with a soft contact lens. As a source of platelets, we used the blood of healthy volunteers, from which platelet-rich plasma with platelet concentration of over 1000 thousand/µl was taken, which was then frozen at -40 °C and defrosted at 0…4 °C to obtain PRP lysate. The criteria for evaluating the effectiveness of both groups were the times of epithelialization, the number of amniotic membrane coatings, and the number of preserved transplants at the end of the follow-up. Results . Lyophilized amniotic membrane saturated with autologous PRP lysate growth factors was shown to be biocompatible. It was found to be safe for the patients, to reduce the epithelialization time, to reduce the number of operations required to cover the PÑE with the amnion, and to increase the likelihood of successful transparent and translucent engraftment of a penetrating keratograft. Conclusion . The use of a lyophilized amniotic membrane enriched with growth factors of autologous PRP lysate is a promising method for the treatment of PCE of the penetrating corneal graft after high-risk keratoplasty.

Purpose: to evaluate the effectiveness of silicon-dried cornea, previously subjected to corneal collagen UVA crosslinking, for urgent keratoplasty.Material and methods. 28 patients (16 males, 12 females), diagnosed with perforating corneal ulcer of various etiologies, underwent penetrating keratoplasty for eye-preserving purposes. 14 patients (the main group) underwent transplantation of a silicon-dried cornea, previously subjected to corneal collagen crosslinking according to the standard (Dresden) protocol while the remaining 14 patients (comparison group) underwent penetrating keratoplasty with not pre-treated silicon-dried cornea. In the postoperative period, we measured the time of primary complete epithelization of the graft and assessed the presence/absence of ulcers and erosions of the corneal graft 1, 3, 6 and 12 months after the discharge from the hospital. For this purpose, all patients underwent corneal biomicroscopy with corneal surface preliminary stained with a 1% sodium fluorescein solution.Results. Complete epithelization in both the main group and the comparison group was found to take place, on average, at the same time after surgery. However, over the entire observation period, the main group showed fewer relapses of ulcers and corneal graft erosions than the comparison group.Conclusion. The use of silicon-dried cornea, subjected to corneal collagen cross-linking, increases the effectiveness of perforated corneal ulcer treatment in emergency surgical care.

Purpose: to study the effectiveness of the use of thrombofibrin clot of platelet-rich plasma (PRP) in patients with corneal ulcers. Material and methods. A clinical study, conducted by the Department of Traumatology and Reconstructive Surgery of Helmholtz National Medical Research Center of Eye Diseases, involved 20 patients, aged from 22 to 82, with corneal ulcers of inflammatory and burn genesis more than 100 microns deep. All patients got coated with a thrombofibrin PRP clot from autologous blood. Prior to the study, all patients received standard treatment for 2 weeks to 3 months, including multiple amniotic membrane coating, with no effect. The thrombofibrin clot was produced by the Scientific Department of Biotechnology and Transfusiology of the N.V. Sklifosovsky Research Institute for Emergency Medicine. The ready clot was placed on the surface of the cornea and covered with an amniotic membrane. The membrane was fixed to the episclera along the border of the limb with a circular suture, whereupon autologous serum was injected along the limb in 4 quadrants, to be followed by temporary lateral blepharography. Results. On the 2nd day following the procedure, the patients noted a decrease in lacrimation and pain in the operated eye. As shown by optical coherence tomography, the average depth of the corneal ulcer at the beginning of the study in all patients was 129 ± 28.5 microns. On the 5th day, the depth lowered to an average of 71 ± 32.6 microns, and on the 10th day, to 23.3 ± 15.1 microns. In 7 patients (35%), complete healing of the defect was observed on the 15th day, while in 9 patients (45%) it was stated between the 16th and the 20th day. Thus, the average time of healing of the ulcer with complete epithelization occurred was 15 days. In four patients with the consequences of severe burns (20%), the ulcer did not heal due to extensive damage to the limbal zone. Conclusion. The use of a thrombofibrin PRP clot in combination with amniotic membrane transplantation allows achieving a stable and fairly rapid healing of corneal ulcers of various origins. However, this method is ineffective in patients with limbal cell insufficiency, severe burns and extensive damage to the limbal zone. In such cases, it is advisable to use more radical surgical methods, such as buccal or limbal cell transplantation, or allolimbal transplantation.

Purpose: To study the biological effect of a combination of platelet lysate and amniotic membrane, preserved by various techniques, on human buccal epithelium culture. Materials and methods. Human amnion transplants were preserved using 3 methods: silicate drying, lyophilization, cryopreservation. The blood of healthy volunteers was used as a source of platelets. Platelet-rich plasma (PRP) with a platelet content over 1000 thousand/mcl and more was isolated from the donors blood, frozen at -80 °С and defrosted at 0–4 °С to prepare platelet lysate. Growth-stimulating effect of the amnion transplants was studied in different groups: control group 1 — without amnion and without PRP lysate; control group 2 — PRP lysate without amnion; experimental group 1 — amnion without PRP lysate; experimental group 2 — amnion samples combined with PRP lysate. The study was carried out on the example of human buccal epithelium culture of 3–5 passages. The dynamics of cell growth was evaluated after 1, 2 and 3 days from the moment of seeding. The number of cells and their viability were evaluated using original methods based on vital cell staining and their examination in a fluorescent microscope. Results. All samples of preserved amnions were non-toxic and did not damage the structural and functional characteristics of the buccal epithelium. On the other hand, the use of amnion without PRP lysate did not have a growth-stimulating effect on cells. Among the amnion samples combined with PRP lysate, the combination of lyophilized amnion and PRP lysate was the most effective during the entire study period. Conclusions. Silicate drying, lyophilization and cryopreservation of the amniotic membrane makes it possible to obtain biocompatible and non-toxic transplants, based on human amnion. Lyophilized amnions are the most optimal for saturating PRP lysate. The combination of lyophilized amnion and PRP lysate stimulates cell growth in vitro without violating their structural integrity.

Purpose. Of the work is to compare the effectiveness of treatment of burn ulcers with canned amnion and amnion saturated with lysate of autologous platelet-rich plasma (PRP). Material and methods. To compare the effectiveness of treatment of canned amnion and lysate-soaked PRP, 10 patients with ulcers with an area of more than ½ of the cornea after fresh burns of 2–3 degrees aged from 26 to 53 were observed, divided into 2 equal groups. In the comparison group, the treatment of ulcers was carried out against the background of conservative treatment by covering the cornea with an amniotic membrane for 7 days, after which the amnion was removed and the condition of the cornea was monitored. If necessary, the procedure was repeated. In the experimental group, an amniotic membrane saturated with lysate of autologous PRP was used. The assessment of the condition of the cornea was carried out using clinical and instrumental studies, including visometry, biomicroscopy with fluorescein staining, photoregistration and OCT on the Heidelberg Engineering “OCT Spectralis” apparatus. Results. In patients of the experimental group, transplantation of an amniotic membrane impregnated with PRP lysate, in comparison with conventional amnion, led to epithelization of ulcers faster by an average of 7 days, the cornea remained more transparent, and visual acuity was higher by 0,17. Conclusions. The use of an amniotic membrane saturated with PRP lysate may be a promising method of treating corneal burn ulcers. Keywords: amnion; platelet-rich plasma; platelet lysate; corneal ulcers; corneal burns.

The actual problem of treatment of patients with limbal cell stem deficiency is reviewed. We summarized practical experience in various fields of medicine, aimed at tissue reconstruction using cells of the buccal mucosa. In ophthalmology, an effective method has long been searched to treat patients with the limbal stem cell deficiency causing an intense opacification and vascularization of the cornea and followed by a significant decrease in visual acuity. Recent studies have shown that the transplantation of epithelial cells of oral mucosa can significantly improve the treatment of patients with this disease. Although the mechanisms of oral mucosa epithelial cells' action are still insufficiently studied, the existing positive experience of oral mucosa using for tissue repair has great interest to practitioners, giving potential possibilities of its use, therapeutic effectiveness and ease of obtaining. A brief review of the literature presents the description of the morphological features of the. buccal mucosa and the analysis of published data about the use of buccal epithelium in various branches of medicine and in ophthalmology, in particular.

Purpose: to evaluate the results of spontaneous non-penetrating keratoprosthesis with a Fedorov — Zuev prosthesis in a patient with severe chemical burns in both eyes. Material and methods.Patient K., 38, who had sustained a severe burn injury in the past and numerous reconstructive plastic operations on both eyes (amniotic tissue implantation, allolymbal transplantation, layer-by-layer and penetrative keratoplasty, cataract extraction with IOL implantation, total auto-conjunctival corneal plastic surgery) with no functional effect, was subjected to a multi-stage keratoprosthesis of the left eye according to the method practiced by the Helmholtz National Medical Research Center of Eye Diseases. Results.After one of the stages (implantation of the haptic part of the keratoprosthesis with a temporary cylindrical plug), an aseptic necrosis of the tissue above the cylinder occurred. As a result, an unexpected functional effect was revealed: visual acuity of the operated eye 0.02 sph -20.0 D = 0.2. During a dynamic follow-up that lasts 2.5 years, visual acuity remains stable, and the corneallayers behind the cylinder retain transparency. During this time, all stages of keratoprosthesis were performed on the fellow eye with a functional result of 1.0. Conclusion. The long-term result of spontaneous non-penetrating keratoprosthesis indicates the need to study the prospects and develop a method of non-penetrating keratoprosthesis with a Fedorov — Zuyev prosthesis.

Purpose:to assess the effect of ultraviolet (UVA) crosslinking of Bioplast plastic material (silicon-dried human cornea) on its biomechanical stability and effectiveness for keratoplasty in corneal ulcers. Material and methods. To determine the maximum rehydration time, 12 Bioplast samples were incubated in physiological solution (NaCl 0.9 %) for 12 hours and monitored for thickness using optical coherence tomography (OCT Spectralis, Heidelberg Engineering). After the epithelium was scarified rehydrated corneas were treated withof 0.1% riboflavin solution for 30 minutes, and after it 8 rehydrated samples (experimental group) were treated with UVA for 30minutes (wavelength 370 nm, power 3 mW/cm2) using a UV-X device system (IROC AG, Switzerland). In the control group (4 rehydrated samples), no UVA crosslinking was performed. The elastic-strength indicators of the experimental and control group samples were assessed by applying uniaxial tension using a deformation machine (Autograph AGS-H, Japan). To examine the patients (presented in clinical examples)before and after keratoplasty with Bioplast subjected to UVA crosslinking, a set of standard ophthalmic tests was used. Results.Rehydration of Bioplast was the most intensive in the first three hours of incubation, during which time tissue thickness increased uniformly by 50–55 μm/hour to 700–710 μm, while further incubation showed no significant additional effect on the fluid content in the cornea. After UVA crosslinking, the strength indices and the elastic modulus of the rehydrated Bioplast samples significantly increased in comparison with the control group. Clinical examples demonstrate the effectiveness of modified Bioplast for organ-preserving penetrating keratoplasty in case of perforatedcorneal ulcer. Conclusion. UVA crosslinking of rehydrated Bioplast increases its biomechanical stability. The data of laboratory studies and the first clinical tests demonstrate that modified Bioplast may be an effective replacement of native donor cornea in urgent keratoplasty of perforated ulcers.

Purpose: to evaluate the effectiveness of platelet-rich plasma (PRP) lysate in patients with corneal erosion or posttraumatic scarring of eyelid tissues.Material and methods. The study involved 30 patients aged 22 to 82 (51.5 ± 19.1 years): 10 with persistent corneal erosions, who were treated in 2 stages (2 weeks of standard keratoprotective therapy, then treatment supplemented with instillations of PRP lysate) and 20 patients with post-traumatic scarring of eyelid tissues, who were divided into a clinical group and a comparison group. PRP lysate was produced in the cell transplantation and immunotyping laboratory of N.V. Sklifosovsky Research Institute for Emergency Medicine.Results. In group 1, already on the 3rd day of PRP lysate use, a positive dynamic of the cornea was observed: all patients had fewer complaints of pain, photophobia, and lacrimation. Corneal biomicroscopy, performed daily over the entire epithelization period, revealed a decrease in hyperemia of the tarsal and bulbar conjunctiva and corneal edema, which indicated a weakened inflammatory response. On average, the area of the cornea at the start of traditional therapy was 21.6 [19.6; 30.65] mm2. After two weeks of treatment, at the time of adding PRP lysate, the average area of erosion fell to 15.7 [12; 18.9] mm2, after a week of treatment with lysate, it dropped to an average of 2.35 [0.4; 4.3] mm2. On day 14, all patients revealed complete epithelialization of the corneal defect. In patients with post-traumatic eyelid tissue scars, after a 3-month-long use of PRP lysate (study group), an improvement was noted in the cosmetic and functional state of the eyelids, on average by 4 points (Me = 12 [10; 12] points before and 8.5 [8; 9] points after treatment (on the Manchester scar assessment scale), in contrast to the patients who received no anti-scar therapy (comparison group), as the latter showed a deterioration in the condition of eyelid scars by 1–2 points (Me = 11 [10.25; 11.75] points at the beginning and 12 [11; 12] points at the end of the observation period). The acoustic density of scar tissue in the study group approached the normal values, in contrast to the comparison group, in which scar tissue appeared to be hypoechoic.Conclusion. Using PRP lysate aimed at improving the repair and regeneration processes in patients with corneal erosion or post-traumatic scarring of eyelid tissues is promising and requires further clinical study.

В настоящее время в структуре глазного травматизма увеличилась доля контузионной травмы, которая характеризуется особой тяжестью и может приводить к гибели глаза. Детальное исследование функционального состояния глаза при его контузии необходимо для определения степени поражения внутриглазных структур с целью оценки выраженности и уровня повреждения, прогнозирования течения патологического процесса и возможных исходов травмы глаза, определения тактики и оптимальных методов консервативного и хирургического лечения. Проблема диагностики контузионной травмы является актуальной и нерешенной и в настоящее время. В связи со многими факторами: тяжестью патологии, поздним обращением больного в стационар, недостаточно высоким качеством клинического и инструментального обследования, значительно сужается возможность проведения патогенетически направленных оперативных вмешательств, адекватно решающих вопросы восстановления правильных анатомических взаимоотношений в травмированном глазу. Несмотря на это, изучение этиологии травмы приводит к поиску новых подходов лечения травматических повреждений органа зрения, как медикаментозного, так и хирургического, и создания фона наилучшего благоприятствования для подхода к лечению пострадавшего.