A chimeric recombinant protein PSH was composed of two antioxidant enzymes, human peroxiredoxin 6 (Prx6) and superoxide dismutase (MnSOD) from Escherichia coll. Analysis of physico-chemical properties of PSH protein confirmed its high antioxidant activity. PSH was shown to protect animals from lethal and sub-lethal doses of ionizing radiation. PSH was most effective after intravenous administration short time before ionizing irradiation. Dose reduction factor for PSH comprised 1.33. PSH was effectively alleviating the degree of radiation-induced leucopenia and thrombocytopenia in animals. Beside that, PSH administration decreased DNA damage in red bone marrow cells. The chimeric recombinant PSH can be considered as an effective radioprotector for minimizing the risks of damage of animal's body by ionizing radiation, and it could be a promising therapeutic molecule for prevention/suppression of pathological conditions accompanied or caused by oxidative stress.

Acute ultraviolet (UV) -induced skin damage is associated with structural alterations, vasodilatation and inflammatory response. Leukocyte infiltration is one of the main features of inflammation and could be found in the area of UV injury. It was shown that porphyrins which have well-known autofluorescent properties play a role in the chemoattraction of immune cells to the area of local damage. This study examined the possibility of application of laser fluorescence spectroscopy (LFS) in the assessment of ultraviolet-induced immune response in ICR mice. Animals (N=25) were exposed by UVB light and LFS was conducted on the dorsal skin of each mice 0, 0.5, 3, 6 and 24 hours after UV irradiation. Moreover, in every time point we performed skin biopsy and histology. Using LFS, time-dependent dynamic changes in the fluorescence parameters of porphyrins were found. Mentioned indices were in a good agreement with histological findings. Statistically significant correlation was found between the severity of inflammatory infiltrate and the tissue content index (η) of porphyrins (Pearson correlation coefficient: r = 0.912, p = 0.031). Achieved results not only have fundamental value but could be further investigated and applied in clinical practice: e.g. to objectively predict individual immunologic reaction to UV-light.

It is known that neurotic disorders can be independent risk factors for the development of complications in patients with cardiovascular diseases. This requires the creation of new rehabilitation programs that include not only medicines but also physiotherapy. To this end, an analysis was made of the effect of complex treatment, including visual color impulse therapy, on the psychoemotional state of 80 patients with cardiovascular diseases, hypertensive disease and coronary heart disease, who also have asthenic-depressive symptoms. In this case, patients in two control groups received standard drug therapy, and in the two main groups received additional color pulse therapy from the «Mellon» apparatus. Dynamics of the psychoemotional state of patients was studied with the help of a test that determines the quantitative changes in the state of health, activity and mood, as well as the stress index that characterizes the regulation of the heart rhythm under stress. Prior to the start of treatment, functional disintegration of regulatory mechanisms was characteristic for all patients, which was accompanied by psychoemotional disorders that indirectly reflected the state of highly organized nervous processes in the cerebral cortex, which altered the values of SI stress index indicators and the «well-being, activity, mood» test. The regression statistical analysis was used in this work. As a result, the indications and contraindications to the use of this method of treatment were determined. After the course of complex treatment, including drug and visual color impulse therapy, positive dynamics in the patients’ state was revealed, which indicates an increase in the functional capabilities of their body, and also, given the good tolerability of visual color impulse therapy, the feasibility of including this method in complex treatment at any stage of rehabilitation.

Aim: To assess the kind of damage of musculoskeletal apparatus of eye and orbit. To develop algorithm of laser therapy to restore orbital circulation and algorithm of transcranial laser therapy to improve microcirculation in maxillofacial muscles. Materials and methods: 75 patients with midfacial trauma were examined using laser spectrophotometry and computer modeling of orbital structures in frontal, sagittal, and inclined planes with SSD, MPR and VRT reconstructions (with Mimics software (Materialise, Leuven, Belgium)). To study orbital and maxillofacial microcirculation complex Spectrotest was used. Tissue oxygen concentration (SatO2) and blood filling volume index of microcirculation (Vbf) were measured. Results: After computer modeling, several variants of damage of musculoskeletal apparatus of eye and orbit were identified including oculomotor muscle entrapment in 60% of patients, angular deviation of muscle belly in 30%, muscle contusion – in 45%, orbital hematoma – in 18%, scarring of musculoskeletal apparatus of orbit – in 40%. During early period of trauma of orbital wall and maxillary sinus, Vbf decreased by a mean of 12.5 ± 0.5% compared to normal values, whereas contralateral microcirculation increased by a mean of 28.5 ± 0.1% compared to normal values. Abnormal blood distribution persisted in the long-term, after bone reposition: blood flow increase was found only in 10% of patients. In severe trauma and chronic scarring, local laser irradiation was associated with increase of Vbf bilaterally, but chronically and significantly increased SatO2 indicated impaired tissue trophics and high risk of inflammatory complications. Conclusion: Computer modeling is of great value in the diagnosis and rehabilitation planning in patients with injuries of musculoskeletal apparatus of eye and orbital structures. Laser spectrophotometry is useful for the monitoring of microcirculation before, after the operation and during rehabilitation.

Reactive arthritis is an autoimmune disease from the group of seronegative arthritis known as spondyloarthropathy. Two types of the disease are designated by this term, namely post-enter- itic (Reiter's syndrome) and venereal (urogenital) (Reiter's disease). Irrespective of the infectious agent, both types of the disorder can be associated with various symptoms including skin and mucosal lesions and heart. However, the post-enteritic type of reactive arthritis is associated also with intestinal problems and the venereal one (mostly being of chlamydial origin), – with gen- itourinary disorders. Due to highly variable clinical manifestations of reactive arthritis, patients frequently receive only symptomatic treatment that temporarily eliminates some symptoms. We describe a clinical example of diagnostic approach to this complex disease, that allowed for putting the defnite diagnosis and perform diferential diagnosis with other similar disorders including urogenital reactive arthritis. This paper could be of interest for practitioners in various specialties, frst, for dermatovenereologists, rheumatologists, gastroenterologists, internists and rehabilitation specialists.

Background: Localized scleroderma often results in irreversible cosmetic abnormalities and if advanced, to organ dysfunction and disability. Pharmacological treatment is not effective enough and can cause adverse events and complications. Due to this fact, much attention is paid to non-medical approaches to this disorder that would be able to exert positive clinical and patho-physiological effects and potentiate the effects of medical treatment. Aim: To evaluate the effects of local long-wave infrared irradiation on the course of localized scleroderma and to assess therapeutic efficacy of this method combined with conventional pharmacological treatment. Materials and methods: Fifty five (55) patients with localized scleroderma were included into the study. The patients from the control group (n = 20) were administered conventional medical treatment for 14 to 20 days, including antibacterials, proteolytic enzymes, calcium antagonists, antioxidants, topical vasculoprotectors + microcirculation correctors, as well as applications of corticosteroids. The patients from the main group (n = 35), in addition to conventional medical treatment, were administered local long-wave infrared irradiation of the lesions (wavelength 4 to 16 mcm, once daily for 10 days). We assessed changes in edema and hyperemia on the borders of the lesion, the degree and extent of the process with a modified scoring system mRODNAN Skin Score, as well as microcirculatory vasculature by means of Spectrotest. Results: Compared to the control group, the patients with localized scleroderma who, in addition to conventional medical treatment, were administered local long-wave infrared irradiation, showed a bigger reduction of mRODNAN index (2.2 ± 0.1 and 6.3 ± 0.5, respectively, р < 0.05), of oxygen saturation within the lesions (0.646 ± 0.04 and 0.9 ± 0.001 units, р < 0.05) and an increase of blood flow volume in the microcirculation (0.23 ± 0.045 and 0.091 ± 0.002 units, р < 0.05). They also demonstrated a quicker clinical improvement that was obvious at day 14 ± 2.3 (р < 0.05). In the control group, the clinical improvement was seen only at day 20 ± 4.2 (р < 0.05), with only a tendency towards a decrease in oxygen saturation within the lesions (from 0.87 ± 0.01 to 0.817 ± 0.005) and an increase of blood flow volume in the micro-circulation (from 0.086 ± 0.004 to 0.1 ± 0.003 units). The conventional medical treatment did not result in full resolution of the local scleroderma elements, this was confirmed by the results of assessment with mRODNAN index (mean value before treatment in this group was 6.4 ± 0.5, after treatment, 5.3 ± 0.2, р ≥ 0.05). Conclusion: The study proved the clinical efficacy of local long-wave infrared irradiation in the combination treatment of localized scleroderma. This effect was seen in the normalization of microcirculation parameters, increase of blood flow, decrease of oxygen saturation within the lesions reflecting active reparation, as well as in better clinical efficacy and more quick effect.