Stress effects are factors that provoke a wide range of socially significant diseases. We studied the effect of two such factors - weak static magnetic field (WSMF) with induction value of 200 mcT and a number of epigenetic regulators, oligopeptides. One of the main cellular processes - proliferation - is shown to occur when WSMF is acting in tissues of various genesis with different intensity. The greatest effect of WSMF exposure is observed in ectodermal genesis tissue - the cerebral cortex. The results of experiments with tissue-specific oligopeptides were comparable to those of WSMF. Analysis of the results of experiments to study cognitive behavior leads to the conclusion of beneficial effects on medium-term memory formation using oligopeptides Ala-Glu-Asp-Pro and Lys-Glu-Asp-Ala. Similarity in the outcome of WSMF and bioregulatory peptides impact in different species of animals on the proliferative cell activity in nervous tissue culture and cognitive behaviour suggests the existence of a common ancient mechanism for regulating the activity of organisms. It is noted that the efficiency of external WSMF influence on biological objects is caused by its high penetration ability.

Metabolism disorders of L-kynurenine, which is an intermediate product of the decomposition of genetically encoded L-tryptophan amino acid, is a component in the development of a number of neuropathological processes. The effect of tryptophan and kynurenine on cell proliferation has been studied in the organotypic culture of cerebral cortex tissue in young and old rats. Tryptophan at an effective concentration (0.05 ng/mL) oppressed cell proliferation in the cerebral cortex in young and old rats by 35 and 18%, respectively. However, cell proliferation was stimulated under the action of kynurenine (1 ng/mL), and it was more pronounced in cerebral cortex explants in old rats (by 22% as compared with the control group). The obtained data on kynurenine properties for the stimulation of cell proliferation in the cerebral cortex in old animals is promising for the creation of new medicinal nootropic drugs in age-associated diseases of the nervous system.

The effects of weak static magnetic field on the organotypic tissue culture of the rat cerebral cortex, liver, and spleen have been investigated. Exposure to a 200 μT static magnetic field induces tissue development, leading to the intensification of regeneration processes compared to the control explants.

Effect of carboxylic acids - structurally related to amino acids, on the proliferation activity of the cells in organotypic cultures of brain cortex (ectodermal genesis) and liver (entodermal genesis) tissues was first studied in young and old rats. It was found that some aliphatic carboxylic acids have stimulating effects on proliferative activity of cells. Only acetic acid inhibited proliferation. In contrast many amino acids were able not only stimulate, but also inhibit cellular proliferation in the explants of young and old rats. This reflects the biological regularity, that the structure amplification leads to increase of the specificity. A decrease of number of active carboxylic acids and amino acids was observed in the explants of old rats. The carboxylic acids preserved the stimulating proliferation activity in the old rat. The data obtained create basis for the synthesis of new medicines, including the carboxylic acids, for increase of the regeneration processes by tissue pathology, also associated with aging.