Existing osteoplastic materials based on osteoconductive matrices lose their relevance. In this connection, to find the most effective and biocompatible carriers for osteoinductors delivery is an important task. Among the existing growth factors BMP-2 is the most effective and researched. Highly porous polylactide (PLA) granules and granular deproteinized bone material Bio-Oss were studied as carriers. Highly porous PLA granules were more effective than Bio-Oss. When using the minimum effective dose of BMP-2 for rats of 10 μg/ml impregnated in highly porous PLA granules, osteogenesis was induced in the calvarial critical-size defect and 18 ± 8% of the defect was filled with a new bone. There were no inflammatory reactions in the PLA granules implantation area. During subcutaneous implantation in rats it was shown that giant multinuclear cells took part in the resorption of the material. Their number was statistically significantly higher in the PLA granule implantation area than Bio-Oss and amounted to 71 ± 23 versus 30 ± 8 cells per 1 mm2. The obtained data showed that highly porous PLA granules were a promising basis for osteoinductive osteoplastic materials.

The study aimed to demonstrate the biocompatibility and osteoinductive properties of a hydrogel based on highly purified collagen and fibronectin impregnated with rhBMP-2. In vitro and in vivo experiments have shown that the minimum effective dosage of rhBMP-2 is 10 μg/ml. The cytocompatibility of the collagen-fibronectin gel was determined using MTT test and staining with PKH-26. There was no inflammation reaction when the material was subcutaneously implanted in rats (n=30) in vivo. The collagen-fibronectin hydrogel containing 10 μg/ml rhBMP-2 showed high osteogenic properties. By the end of 28 days 8±4% of its volume was replaced by newly formed bone tissue in case of subcutaneous implantation, 17±10% in intramuscular implantation and 26±11% in intraosseous implantation in the calvarial critical-size. The optimal combination of biocompatible and osteogenic properties of collagen-fibronectin hydrogel impregnated with BMP-2 allows us to consider it as a promising basis for creating the new generation of osteoplastic materials for dentistry.

In the review, the structure and biological properties of collagen, variants of its production from natural sources and purification are considered. Methods for modifying the physico-mechanical properties of collagen to create a curable, highly purified collagen hydrogel are described. The advantages of a cured highly purified collagen hydrogel as a basis for osteoplastic material and a means of delivery of growth factors are indicated. The registered osteoplastic materials based on the curable highly purified collagen hydrogel are described, and their comparative analysis is carried out. On the basis of the obtained data, a conclusion was made about the prospects of using collagen as a basis for curable and activated osteoplastic materials.

AIM:To compare the cytocompatibility of osteoplastic materials used in dentistry with stem cells from human exfoliated deciduous teeth (SHED) and adipose tissue-derived mesenchymal stem cells (AD-MSC).MATERIAL AND METHODS:Materials of the brands 'Bio-Oss', 'Indost', 'Bioplast', 'Viscoll' and 'Trikafor' were selected for study purposes. Cultures of SHED and AD-MSC were used for testing. The cytotoxic effect of the materials was determined using MTT test and vital staining with trypan blue. Cell adhesion was assessed by the vital staining of PKH-26.RESULTS:Water extracts of bone-plastic materials from xenogeneic hydroxyapatite of the brands 'Bio-Oss', 'Indost' and 'Bioplast' exert a cytotoxic effect on SHED and do not cause the death of AD-MSC. Materials based on collagen and β-tricalcium phosphate possess high cytocompatibility with all cell cultures under study.CONCLUSION:From the point of cytocompatibility all the examined bone-plastic materials may be considered safe for the restoration of bone defects. It should be noted that SHED transplantation on the surface of materials containing xenogeneic hydroxypatite is unacceptable.