We have analyzed the influence of the (tetraphenylporphyrin (TPP), 3,5-di-tret-butil-4-hydroxyphenylporphyrin (TPP-OH)) on carbonylic products accumulation identified with the help of thiobarbituric acid in the sperm of the Russian sturgeon (Acipenser gьldenstдdti Brandt) in vitro in the presence of Hg(NO3)2, CH3HgI, CdCl2 and (CH3)2SnCl2 in conditions of long running oxidation. It was shown the reduction of the rate of the peroxidation of lipids of sturgeon sperm in the presence of studied porphyrins under conditions of auto-oxidation and at promoting the process of heavy metals combination, that gives the possibility to use porphyrin systems as antioxidants. The greater efficiency of TPP-OH in comparison with TPP was observed at all stages of investigation both in conditions of auto-oxidation and in the presence of heavy metals compounds.

It is shown that the promoting effect of a mixture of crude oil and dimethyltin dichloride on the oxidation of the sturgeon's liver homogenate lipids is higher than the effects of these compounds used separately in the same concentrations under conditions of long running oxidation. For the first time the detoxification effect of [(3,5-di-tert-butyl-4-hydroxyphenyl) hydroxymethyl] diphosphonic acid phosphoryl compound containing sterically hindered phenol fragment in comparison with standard antioxidant 2,6-di(tert-butyl)-4-methylphenol (ionol) is considered. The greater efficiency of antioxidative action for the novel antioxidant of multifunction action is observed in autoxidation of the sturgeon's liver homogenate lipids as well as in conditions of promotion of this process by a mixture of the crude oil and dimethyltin dichloride.

We have analyzed the influence of the (tetraphenylporphyrin (TPP), 3,5-di-tret-butil-4-hydroxyphenylporphyrin (TPP-OH)) on carbonylic products accumulation identifying with thiobarbituric acid malondialdehyde (MDA) in liver of the Russian sturgeon (Acipenser guldenstadti Brandt) in vitro in presence of Hg(NO3)2, CH3HgI, CdCl2 and (CH3)2SnCl2 in condition of long running oxidation. It was shown the reduction of promoting action of mercury, tin and cadmium compounds on lipids peroxidation in sturgeon liver in presence of porphyrines additions. The greater efficiency was observed for TPP-OH in comparison with TPP. The efficiency of antioxidative action of studying porphyrines depends on duration of the oxidation process as well as chemical nature of porphyrines. Thus, promoting action of mercury, tin and cadmium compounds of the sturgeon liver lipids peroxidation, as well as decreasing of promoting action of lipids peroxidation at presence of porphyrines confirms that high toxic effect of heavy metal can be connected with their involving into radical processes.

The influence of the most toxic of methyl derivatives of tin thrimethyltin chloride (СH3)3SnCl on hemin catalase activity, was studied. Hemin is a catalytic centre of important hemproteids. It is established that the addition of this toxicant decreases the efficiency of hemin catalysis of hydrogen peroxide decomposition reaction in the alkaline media. Thereby, the toxic action of thrimethyltin chloride on hemproteids of antioxidative protection of fermentative section can be caused by their action on unprotein part of these important proteins, and it promotes the development of antioxidative stress in a living organism.

The influence of mercury and tin compounds (HgCl2, CH3HgI, (СH3)3SnCl) on antioxidant state of hemolysate of blood red corpuscles in vitro is studied in the article. It is stated that the addition of these compounds decreases the speed of enzymatic decomposition reaction of hydrogen peroxide by catalase; moreover it activates the oxidation of adrenaline in alkaline media. (CH3)3SnCl shows the most toxic action. Thereby, the toxic action of mercury and tin compounds can be conditioned by decreasing of antioxidant hemolysate defence system of blood red corpuscles in vitro, and it assists the development of antioxidant stress in a living organism.

The effect of HgCl2, CH3HgI, 3,5-di-tert-butyl-4-hydroxyphenylmercury chloride, (CH3)3SnCl on the initial rate of the hydrogen peroxide oxidation with catalase of washed blood's erythrocytes was studied. It was stated that with addition of studying mercury and tin compounds the reduction of hydrogen peroxide flow rate exists with HgCl2 (in 15 times), 3,5-di-tert-butyl-4-hydroxyphenylmercury chloride (in 5 times), (CH3)3SnCl (in 2 times). The introduction of CH3HgI and 3,5-di-tert-butyl-4-hydroxyphenylmercury chloride in washed blood's erythrocytes rads increases of absorbance and can influence on this toxicant directly to reactions substrate the hydrogen peroxide (it was stated the change of H2O2 spectrum in presence of these compounds).

Influence of inorganic tin (SnCl2), organic derivatives from tin (trimetyl and tributyltin chlorides) and tetraphenylporphyryn on rat's spleen morphology has been studied. There has been found that morphological changes in rat's spleen can be seen as degeneration of erythrocytes, suppression of proliferation of semistem cells of blood and unipotent cells-predecessors, as well as hampering development of lymphoblasts (= lymphogenetic cells). The most evident changes appear when adding organic derivatives of tin into the fodder, especially butylous derivatives of tin. Adding tetraphenylporphyrin into the fodder stimulates rat's immune system.

Influence of inorganic tin (SnCl2), organic derivatives from tin (trimetyland tributyltin chlorides) and tetraphenylporphyrin onto the morphology of liver and pancreas tissues, onto the rate of peroxide oxidation of lipids (POL) in the rat's liver has been studied. There has been found correlation of increasing POL in rat's liver with the irregular changes in morphology of liver tissues and pancreas. According to the rate of promoting peroxide oxidation of lipids in rat's liver and the rate of pathological changes in rat's digestive glands, the tested compounds can be seen as following activity line: Bu3SnCl >> Me3SnCl > SnCl2. The highest rate POL was observed in rat's liver, the most significant changes being obtained by adding tributyltin chloride into the fodder. Decrease of activity POL under the influence of tetraphenylporphyrin shows antioxidant activity of this compound in vivo, pathological changes in the liver tissues and pancreas not being observed.