Прогресс в области трансплантации аллогенных гемо-поэтических стволовых клеток (ГСК) пуповинной крови неразрывно был связан с развитием банков пуповинной крови, созданных для обеспечения систематического сбора, тестирования, обработки, хранения, организации подбора и выдачи образцов пуповинной крови для трансплантации. Целью исследования была разработка алгоритма работы Московского банка-регистра безвозмездных неродственных доноров ГСК пуповинной крови. В статье охарактеризованы биологические характеристики всех образцов пуповинной крови, внесенные в регистр безвозмездных доноров Московского банка стволовых клеток (на 1 криокон-сервированный образец пуповинной крови). Показано, что небольшое количество безвозмездных неродственных доноров ГСК пуповинной крови в базе данных регистра позволяет подобрать совместимую (6/6) пару донор-реципиент с частотой вероятности 1:151. Предложенный алгоритм работы Московского банка-регистра рекомендуется использовать при создании, усовершенствовании и работе других банков-регистров безвозмездных неродственных доноров ГСК пуповинной крови.
The activity of the Register of unrelated donors of hemopoietic stem cells derived from the umbilical cord blood (CB HSC) stored at Stem Cell Bank of Moscow is analyzed. The biological characteristics of cryopreserved umbilical blood samples for one specimen procured for transplantation are presented. The presence of few unrelated donors of CD HSC in the Register database makes it possible to select a compatible (6/6) donor-recipient pair with a probability of 1:151. The results of the Register activity demonstrate objective difficulties in the choice of compatible HSC donor, explained by high genetic variations in the human population and in other parameters of the donor, eventually essential for his/her selection for the recipient.
The main requirement for the quality of cellular material for clinical use is to ensure that viral control in order to prevent infection of the recipient. The aim of the study was to evaluate the persistence and multiplication of human Herpesviridae in human bone marrow and placentaderived multipotent mesenchymal stromal cells (MMSCsBM and MMSCs-P), and their prevalence among donors MMSCs. Expansion MSCs-BM was held from of 6 donors and expansion MMSCs-P was held from of 25 donors for further clinical application of the standard method. We investigated the expression levels of cell surface markers of MMSCs. The quantitative detection of DNA viruses HHV-1, HHV-2, EBV, CMV, HHV-6 was investigated by PCR to the expansion and achieving a sufficient number of MMSCs for clinical use (2-5 passage). In 2 the bone marrow donors was found DNA EBV and HHV-6. These donors were diagnosed lymphohistiocytosis and biliary cirrhosis. In the placenta donors and MMSCs-P were detected DNA EBV and HHV-6 in 2 cases. DNA CMV, HHV-1 HHV-2 and were not detected in any of the investigated samples. The results showed that careful selection of donors, the algorithm reduces the risk of infection of MMSC, the risk of infection with samples of bone marrow donors is increased if the donor bone marrow are patients with severe chronic diseases; EBV and HHV-6 have a tropism to the MMSCs and are able to multiply in the process of expansion; PCR-investigation on the human Herpesviridae before expansion of cells and when a sufficient number of cells MMSCs for further clinical application allows you to quickly identify infected cell material, evaluation of the quality of MMSCs for further clinical application must include the compulsory study of PCR for the human Herpesviridae.