Background/Aims: The aim of the present study is to investigate whether the single nucleotide polymorphism (SNP) in lipid metabolism related genes would affect the effectiveness of atorvastatin in both Han and Uighur populations. Methods: 200 ischemic stroke patients were treated with atorvastatin. The differences of blood lipid level and their ratios were measured. Six lipid related genes, HMGCR, APOA5, LPL, CETP, LDLR and PCSK9 were selected as candidate genes. And nine SNP loci in these six genes were genotyped by SNaPshot technique. Results: In all patients treated with atorvastatin, the SNP rs662799 significantly affected the ratio of ΔLDL and ΔLDL/LDL (p < 0.05); the SNP rs320 significantly affected the ratio of ΔLDL/LDL and Δ(LDL/HDL)/(LDL/HDL) (p < 0.01) and the SNP rs708272 significantly affected the ratio of ΔLDL (p < 0.05). In Han population treated with atorvastatin, the SNP rs662799 significantly affected the ratio of ΔTG (p < 0.05); the SNP rs320 significantly affected the ratio of ΔLDL/LDL and Δ(LDL/HDL)/(LDL/HDL) (p < 0.01). In Uighur population treated with atorvastatin, the SNP rs2266788 significantly affected the ratio of ΔHDL (p < 0.05); the SNP rs662799 significantly affected the ratio of ΔLDL/LDL (p < 0.05) and the SNP rs708272 significantly affected the ratio of ΔLDL (p < 0.05). Conclusion: Polymorphisms of rs662799 and rs2266788 in APOA5 gene, rs320 in LPL gene and rs708272 in CETP gene had significant association with the effect of the lipid-lowering therapy via atorvastatin calcium on ischemic stroke patients.

Objective To explore the correlations among the serum levels of matrix metalloproteinase-9( MMP-9) and its gene C1562T polymorphism and the stability of carotid arteriosclerotic plaque in Uygur patients with cerebral infarction. Methods Two hundred and thirty-six Uygur patients with acute cerebral infarction were detected by color Doppler ultrasonography,and were divided according to the carotid atherosclerotic plaque stability. The serum level of MMP-9 was measured by enzyme-linked immunosorbent assay, and the MMP-9 gene C1562T polymorphism was detected by PCR-restriction fragment length polymorphism. The results between the groups were analyzed. Results There were 108 cases with equal-echo or low-echo of carotid plaques( vulnerable plaque group) and 128 cases with high-echo of carotid plaques( stable plaque group). The serum level of MMP-9 in vulnerable plaque group [( 357. 83 ± 32. 64) ng / ml] was significantly higher than that in stable plaque group [( 304. 47 ± 38. 91) ng / ml]( P 0. 01). There were no significant differences of the frequencies of genotype CC,CT,TT and allele C,T of MMP-9 gene C1562T between the vulnerable plaque group and the stable plaque group. Conclusion The serum level of MMP-9 may be correlated with the stability of carotid arteriosclerotic plaque in Uygur patients with cerebral infarction,while the MMP-9 gene C1562T polymorphism may have no correlation with the stability of carotid arteriosclerotic plaque in Uygur patients with cerebral infarction.

目的探讨血清基质金属蛋白酶9（MMP-9）水平及基因-1562C〉T多态性与维吾尔族缺血性脑卒中（Is）及其各亚组的关系。方法采用病例对照研究，选择维吾尔族急性IS患者284例。按新的急性脑卒中治疗（TOAST）分型分为动脉粥样硬化血栓形成（AT）组91例、小动脉病变（SAD）组150例和心源性脑检塞（CE）组43例；选择同期健康体检者226例为对照组。采用酶联免疫吸附法检测血清MMP-9水平，聚合酶链反应-限制性片段长度多态性技术检测MMP-9基因-1562C〉T的基因型。结果IS组血清MMP-9水平显著高于对照组（0．308±0．033mg／L比0．087±0．011mg／L，t=7．813，P=0．000），且AT组、CE组显著高于SAD组（0．350±0．030mg／L比0．261±O．029mg／L，t=4．156，P=0．001；0．317±0．043mg／L比0．261±0．029mg／L，t=2．877，P=0．031）。多变量Logistic回归分析显示，血清MMP-9水平增高是Is的独立危险因素（OR：1．012，95％CI：1．007—1．016，P〈0．01）。IS组基因型（x2=3．558，P=0．058）和等住基因（x2=3．567，P=0．059）频率与对照组无显著性差异。AT组和对照组之间基因型（x2=5．097，P=0．024）和等位基因（x2=5．439，P=0．02）频率存在显著差异，其中AT组cT＋TT基因型频率显著高于对照组（24．2％比13．7％）。多变量Logistic回归分析显示，MMP-9基因-1562C〉T多态性并非是缺血性脑卒中的独立危险因素（OR：0．821，95％CI：0．622—1．059，P=0．124），但却是AT的独立危险因素（OR：1．768，95％CI：1．178—2．677，P=0．007）。结论维吾尔族Is患者血浆MMP．9水平增高，以AT亚组增高最为显著；MMP-9基因-1562C〉T多态性与维吾尔族人群IS的发生无明显相关性，但可能与AT型脑卒中相关。

Objective To study the change of serum matrix metalloproteinase 9( MMP-9) and tissue inhibitor of matrix metalloproteinase 1( TIMP-1) in acute ischemic stroke patients( AIS),and analyse the relationship with disease prognosis. Methods 226 AIS patients were enrolled in the investigation. Based on the MESS,patients were divided into different groups according to the levels of severity and prognosis. And 164 physically healthy subjects were selected as controls. The concentration of serum MMP-9,TIMP-1,and MMP-9 / TIMP-1 were determined in all cases after being attacked within 24 hour,day3,day14 and day28 respectively. Results( 1) Serum contents of MMP-9,TIMP-1,and MMP-9 / TIMP-1 in AIS in different stages had significant difference. Serum contents of MMP-9,TIMP-1,and MMP-9 / TIMP-1 in AIS were remarkably higher than controls[( 377. 58 ± 32. 77) ng / ml,( 302. 29 ± 42. 25) ng / ml,( 1. 25 ± 0. 17) and( 67. 38 ± 10. 46) ng / ml,( 100. 68 ± 34. 21) ng / ml,( 0. 67 ± 0. 11),P 0. 01].( 2) Serum contents of MMP-9,TIMP-1,and MMP-9 / TIMP-1 among the light,medium and heavy cases after being attacked in 24hour had significant difference.( 3) Serum contents of MMP-9,TIMP-1,and MMP-9 / TIMP-1 in basically curable cases and remarkably improved cases after being attacked in day28 were remarkably lower than unchanged ones.( 4) The levels of hs-CRP、glucose and LDL-C were correlated with the levels of MMP-9 in AIS. Conclusions The change of serum MMP-9,TIMP-1 and imbalance of MMP-9 / TIMP-1 are significant bioindicators to evaluate the level of severity and prognosis of AIS.

Objective To explore the blood pressure variability among different subtypes of acute ischemic stroke. Methods From July 2009 to July 2011, two hundred and twenty six acute ischemic stroke patients aged (66.2±11.9) years, including 128 males and 98 females, were selected in the department of Neurology and emergency of Urumqi friendship hospital. Based on the criteria and classification of trial of Org 10 172 in acute stroke treatment, these patients were divided into three groups: arterial therothrombosis (AT) group (n=98), cardioembolism (CE) group(n=39), and small artery disease (SAD) group(n=89). And another 226 physically healthy subjects at the age of (65.6±10.8) years, including 140 males and 86 females, were selected as controls. Their levels and variability of blood pressure were investigated by 24-hour ambulatory monitor. Results In AT, CE and SAD group, the 24-hour mean systolic blood pressure [(136.7±11.7), (131.2±12.5), (138.1±15.3) vs (116.7±13.2) mm Hg], 24-hour mean diastolic blood pressure [(77.9±12.5), (70.2±11.4), (77.2±12.7) vs (64.4±11.5) mm Hg], pulse pressure index[(0.46±0.08), (0.42±0.07), (0.45±0.05) vs (0.37±0.07)] and variability of 24-hour diastolic blood pressure [(0.17±0.05), (0.14±0.02), (0.17±0.01) vs (0.11±0.04)] were higher than those in the control group. There were statistical differences in the distribution of blood pressure circadian rhythms between three groups(P0.05). Conclusions The blood pressure variability of acute ischemic stroke patients is obviously higher than that of the healthy population. There are statistical differences in blood pressure variability of different ischemic stroke subtypes.